Construction method of AE (Alveolar Echinococcoisis) animal model realizing liver infection with Echinococcus multilocularis through hepatic portal vein

Abstract

The invention relates to the technical field of AE (Alveolar Echinococcoisis) animal models, and provides a construction method of an AE animal model realizing liver infection with Echinococcus multilocularis through the hepatic portal vein. The method comprises steps as follows: a mouse is anaesthetized and fixed, the abdomen of the mouse is disinfected, the hepatic portal vein is exposed through laparotomy, the mouse is inoculated with the Echinococcus multilocularis through hepatic portal vein puncture, a puncture point is pressed until bleeding is stopped, then the abdomen is closed, the mouse subjected to puncture inoculation and abdomen closure is normally fed after reviving, and the AE animal model realizing liver infection with the Echinococcus multilocularis through the hepatic portal vein is obtained. The AE animal model obtained with the construction method of the AE animal model realizing liver infection with the Echinococcus multilocularis through the hepatic portal vein has the advantages of being close to a natural infection route and high in infection rate; therefore, the good animal model is provided for research of the mouse infection rate and the immune tolerance mechanism in different time periods and under different dosages and a host liver damage mechanism caused by Echinococcus multilocularis infection as well as screening of new drugs for treating AE.

Description

technical field [0001] The invention relates to the technical field of alveolar echinococcosis animal models, and relates to a method for constructing alveolar echinococcosis animal models in which alveolar coccoids infect the liver through the hepatic portal vein. Background technique [0002] 1. The hazards of alveolar echinococcosis and the demand for suitable models [0003] Worldwide, echinococcosis is a serious public health problem. The disease is prevalent in the Middle East, Southern Europe, Latin America, Central Asia, Australia, and some countries in Africa. In my country, Gansu, Xinjiang, Ningxia, Inner Mongolia, Qinghai and other provinces (regions) are the most prevalent, and the incidence rate of the population can be as high as 5% to 10%. %. Among them, Alveolar Echinococcoisis (AE) is a kind of "hepatocarcinoma-like" caused by the larvae (hexacococcus) of Echinococcus multilocularis (Echinococcus multilocularis, E.m) parasitizing the human body. Fatal para...

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Problems solved by technology

[0009] The present invention provides a method for constructing an animal model of alveolar echinococcosis in which alveolar coccoids infect the liver through the hepatic portal vein, which overcomes the above-mentioned deficiencies in the prior art, and can effectively solve the problem of building an animal model of alveolar echinococcosis. The method has the problem that it cannot be close to the natural infection route and has a high infection rate; the present invention discloses for the first time a method for constructing an animal model of alveolar echinococcosis in which alveolar coccoids infect the liver through the hepatic portal vein, which is not only better close to the natural infection route , and the infection rate is high, the infection rate reaches more than 90%, and the number of alveolar coccidia can be accurately inoculated at the same time, for the purpose of studying the infection rate of mice at different times and doses of alveolar coccus, the mechanism of immune tolerance, and the infection caused by alveolar coccus The mechanism of host liver injury and provide a good animal model for screening new drugs for the treatment of alveolar echinococcosis

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