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Method for increasing anthocyanin content of vitis davidii cell cultures

A technology for cell culture and anthocyanin production, which is used in biochemical equipment and methods, plant cells, culture processes, etc.

Inactive Publication Date: 2016-12-21
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, natural proanthocyanidins are mainly derived from pine bark and grape seeds. With the continuous expansion of market demand, the original bioactive substances derived from grapes, pine bark or other alternative plants can no longer meet the needs, and more must be sought. More reliable source of material

Method used

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  • Method for increasing anthocyanin content of vitis davidii cell cultures
  • Method for increasing anthocyanin content of vitis davidii cell cultures

Examples

Experimental program
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Embodiment 1

[0019] (1) Selection and pre-cultivation of grapevine cell lines

[0020] Select grapevine cell lines that show purple stably in the long-term subculture process, and pick cells from the surface layer of the cell mass with a part of the inner layer as the material for inoculation. In order to make the cells grow vigorously and tend to be synchronized, the cells need to be pre-cultured. The specific method is: subculture the cells of the previous generation for 20 days, and pick the cells on the surface of the cell mass with a part of the inner layer according to the above-mentioned method. As the material for inoculation, the cells were inoculated into TO medium (MS+2,4-D 1.0 mg / L) without KT and cultured for 20 days as the starting material for inoculation of high-content anthocyanin cells.

[0021] (2) Cultivation of high-content anthocyanin thorn grape cells

[0022] a. Culture medium

[0023] Six medium formulations with gradient concentration of KT were designed for the...

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Abstract

The invention provides a method for increasing the anthocyanin content of vitis davidii cell cultures. The method comprises the steps that a vitis davidii cell line stably showing purple red color in the secondary culture process is selected from cells which are subjected to previous-generation secondary culture for 20 days, cells of which the surface layers slightly have part of the inner layers are picked to serve as an inoculating material, a culture medium in which KT is not added is inoculated with the picked cells, culturing is conducted for 20 days, and the cultured cells are taken as a starting material for high-content anthocyanin cell inoculating; cells of which the surface layers slightly have part of the inner layers are picked from the starting material to serve as an inoculating material, a culture medium induced by high-content anthocyanin is inoculated with the picked cells, culturing is conducted for 3 days in low light or a dark environment at 25+ / -1 DEG C and then continues to be conducted for 32 days under the condition that illumination is conducted for 12 h every day, and the vitis davidii cell cultures with the high-content anthocyanin are obtained. According to the method, not only can a large quantity of vitis davidii cell cultures be quickly obtained, but also the obtained vitis davidii cell cultures have the high-content anthocyanin, and then a good foundation is laid for natural anthocyanin production achieved through large-scale vitis davidii cell culture.

Description

【Technical field】 [0001] The invention relates to a method for increasing the anthocyanin content of grape cell culture. 【Background technique】 [0002] The thorny grape is a wild species of the East Asian population of the genus Vitis vinifera. The thorn grape fruit is purple-black, extremely nutritious, and contains flavonoids (mainly anthocyanins and proanthocyanidins), resveratrol, oleanolic acid, tannin, superoxide dismutase (superoxide dismutase, SOD) and vitamin C and other natural active ingredients. Studies have shown that the anthocyanins and proanthocyanidins rich in thorn grape fruit have a strong ability to scavenge free radicals, and have the functions of anti-oxidation, anti-mutation, anti-tumor, and cardiovascular protection. It is recognized by the world as the most effective natural antioxidant for removing free radicals in the human body. At present, natural proanthocyanidins are mainly derived from pine bark and grape seeds. With the continuous expansi...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12P17/06
CPCC12N5/04C12N2500/30C12N2500/34C12N2500/40C12P17/06
Inventor 赖呈纯范丽华黄贤贵潘红
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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