Mathematical model and data processing method of high performance liquid chromatography-atomic fluorescence combined analysis system
A high-performance liquid chromatography and atomic fluorescence technology, which is applied in the field of mathematical models and data processing of high-performance liquid chromatography-atomic fluorescence coupled analysis systems, can solve problems such as influence and limited correction of experimental results
Active Publication Date: 2016-12-21
DEZHOU VOCATIONAL & TECHN COLLEGE
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Problems solved by technology
[0010] The above four methods give mathematical models for hydride generation, transmission and detection, but they are all based on the influence of a single variable on the results, and the correction effect on the experimental results is limited.
[0011] At present, the simple Gaussian fitting method is mostly used for the output spectrum, but the HPLC-HG-AFS system is not a separate HPLC system, but a "combined system" of HPLC and HG-AFS system. Therefore, the HG-AFS system at the rear The AFS system will inevitably affect the Gaussian concentration distribution signal output by the HPLC. Therefore, simply using the Gaussian fitting commonly used in the HPLC system and ignoring the influence of the rear HG-AFS system will obviously have a large deviation
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[0040] Such as figure 1 and figure 2 Shown: a high-performance liquid chromatography-atomic fluorescence coupled analysis system, including a high-performance liquid chromatography mechanism, a hydride generating mechanism, a gas-liquid separator, an atomic fluorescence spectrometer, and a computer. The high-performance liquid chromatography mechanism includes a high-pressure pump, six Through valve and sample loop, the hydride generating mechanism is composed of a peristaltic pump group, a mixer and a reactor. The outlet of the reactor is connected with the reactant inlet pipe of the gas-liquid separator, and the reactant inlet pipe is connected obliquely to the separator body, the gas outlet pipe at the top of the separator body is connected with the atomic fluorescence spectrometer, and the lower part of the separator body A waste liquid outlet pipe is arranged on one side, and the atomic fluorescence spectrometer is connected with a computer.
[0041]The working princip...
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The invention provides a mathematical model and a data processing method of a high performance liquid chromatography-atomic fluorescence combined analysis system. The mathematical model comprises a high performance liquid chromatography mechanism, a hydride generating mechanism, a gas-liquid separator, an atomic fluorescence spectrometer and a computer, and the function relation between system parameters and peak shape broadening and sensitivity is established through Gaussian distribution of the HPLC mechanism based on the high performance liquid chromatography-atomic fluorescence combined analysis system. The errors produced by purely adopting the Gaussian fitting method commonly adopted by the HPLC system are effectively decreased, and it is advantageous to later processing of experimental data, interference removal, effective calculation of the peak area for concentration inversion and improvement of the correlation coefficients of a standard curve and the detection accuracy. The peak height, peak width and graph parameters of output signals can be predicted, and the mathematical model has higher guiding significance and practical value for adjustment of instrument working conditions and improvement of instrument parameter indexes of instrument production manufacturers.
Description
technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a mathematical model and a data processing method of a high performance liquid chromatography-atomic fluorescence coupled analysis system. Background technique [0002] Flow injection-hydride generation-atomic fluorescence spectrometry has been widely used in health, environmental protection, geology, metallurgy and other industries due to its many advantages such as high sensitivity, wide measurement range, and fast analysis speed. However, there are many factors that affect the sensitivity and peak broadening of the hydride generation-atomic fluorescence spectrometer. Generally, the best experimental conditions are found through multiple experiments, and the optimization of experimental conditions is difficult. [0003] The Flow Injection-Hydride Generation (FI-HG) method is to introduce the sample through flow injection and react with KBH4 in an acidic environment. T...
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IPC IPC(8): G01N30/02G01N21/64
CPCG01N21/64G01N30/02G01N2021/6417G01N2030/027
Inventor 崔健崔宁陈章侠殷淑英梁强
Owner DEZHOU VOCATIONAL & TECHN COLLEGE