Mathematical model and data processing method of high performance liquid chromatography-atomic fluorescence coupled analysis system
A high-performance liquid chromatography and high-pressure liquid chromatography technology, which is applied in the field of chemical analysis, can solve problems such as influence and limited correction effect of experimental results, and achieve the effects of reducing errors, high guiding significance and practical value, and improving correlation coefficients
Active Publication Date: 2018-11-23
DEZHOU VOCATIONAL & TECHN COLLEGE
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[0010] The above four methods give mathematical models for hydride generation, transmission and detection, but they are all based on the influence of a single variable on the results, and the correction effect on the experimental results is limited.
[0011] At present, the simple Gaussian fitting method is mostly used for the output spectrum, but the HPLC-HG-AFS system is not a separate HPLC system, but a "combined system" of HPLC and HG-AFS system. Therefore, the HG-AFS system at the rear The AFS system will inevitably affect the Gaussian concentration distribution signal output by the HPLC. Therefore, simply using the Gaussian fitting commonly used in the HPLC system and ignoring the influence of the rear HG-AFS system will obviously have a large deviation
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[0039] Such as figure 1 with figure 2 Shown: A high-performance liquid chromatography-atomic fluorescence combined analysis system, including high-performance liquid chromatography mechanism, hydride generation mechanism, gas-liquid separator, atomic fluorescence spectrometer and computer, high-performance liquid chromatography mechanism including high-pressure pump, six The hydride generation mechanism is composed of a peristaltic pump set, a mixer and a reactor. The outlet of the sample loop communicates with the pre-reactor, the peristaltic pump set communicates with the mixer and the reactor, and the outlet of the mixer is connected to the reactor. The inlet of the reactor is connected, the outlet of the reactor is connected with the reactant inlet pipe of the gas-liquid separator, the reactant inlet pipe is connected to the separator body obliquely, the gas outlet pipe at the top of the separator body is connected with the atomic fluorescence spectrometer, and the lower pa...
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The invention provides a mathematical model and a data processing method of a high performance liquid chromatography-atomic fluorescence combined analysis system. The mathematical model comprises a high performance liquid chromatography mechanism, a hydride generating mechanism, a gas-liquid separator, an atomic fluorescence spectrometer and a computer, and the function relation between system parameters and peak shape broadening and sensitivity is established through Gaussian distribution of the HPLC mechanism based on the high performance liquid chromatography-atomic fluorescence combined analysis system. The errors produced by purely adopting the Gaussian fitting method commonly adopted by the HPLC system are effectively decreased, and it is advantageous to later processing of experimental data, interference removal, effective calculation of the peak area for concentration inversion and improvement of the correlation coefficients of a standard curve and the detection accuracy. The peak height, peak width and graph parameters of output signals can be predicted, and the mathematical model has higher guiding significance and practical value for adjustment of instrument working conditions and improvement of instrument parameter indexes of instrument production manufacturers.
Description
Technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a mathematical model and a data processing method of a high-performance liquid chromatography-atomic fluorescence combined analysis system. Background technique [0002] Flow injection-hydride generation-atomic fluorescence spectroscopy has many advantages such as high sensitivity, wide measurement range and fast analysis speed, and has been widely used in health, environmental protection, geology, metallurgy and other industries. However, there are many factors that affect the sensitivity and peak broadening of the hydride generation-atomic fluorescence spectrometer. Generally, it is difficult to optimize the experimental conditions to find the best experimental conditions through multiple experiments. [0003] The flow injection-hydride generation (FI-HG) method is to introduce the sample through the flow injection method and react with KBH4 in an acidic environment. The ana...
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IPC IPC(8): G01N30/02G01N21/64
CPCG01N21/64G01N30/02G01N2021/6417G01N2030/027
Inventor 崔健崔宁陈章侠殷淑英梁强
Owner DEZHOU VOCATIONAL & TECHN COLLEGE