Preparation method of immobilized enzyme used for processing papermaking white water

A technology for immobilizing enzymes and white water for papermaking, which is applied in chemical instruments and methods, immobilization on/in organic carriers, and wastewater treatment during processing, etc. The effect of the de-recycling process

Inactive Publication Date: 2017-01-04
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] Although free enzymes have many advantages, there are many disadvantages at the same time: they are very sensitive to the environment, the price is usually expensive, and it is difficult to recover and reuse from the catalytic reaction system; and it is difficult to separate from the product after the reaction is completed, resulting in product pollution, etc.
However, the composition of papermaking white water is complex, and the temperature of white water is generally high (55-60°C) when it is closed, and enzymes are easily inactivated. These conditions limit the promotion and application of enzymes in the papermaking industry.

Method used

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  • Preparation method of immobilized enzyme used for processing papermaking white water

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preparation example Construction

[0023] A preparation method for immobilized enzymes for processing papermaking white water, comprising the steps of:

[0024] (1) Dissolve cationic polyacrylamide (CPAM) in water to make a solution;

[0025] (2) Adding the pulp fiber to the cationic polyacrylamide solution prepared in step (1), so that the pulp concentration is 0.1-0.8%. Stir for 30-60min, then filter and wash with a Buchner funnel;

[0026] (3) Put the fiber carrier prepared in step (2) into a pectinase solution with a concentration of 0.1-0.25%, so that the slurry fiber concentration is 0.1-0.8%, stir for 30-60min, filter with a Buchner funnel, and wash;

[0027] In order to further realize the purpose of the present invention, the concentration of the CPAM aqueous solution is preferably 0.1-1 g / L.

[0028] The following specific preparation examples are used to illustrate the specific operation method and effect. In the present invention, all percentages are by weight if there is no special description. ...

Embodiment 1

[0030] A preparation method for immobilized enzymes for processing papermaking white water, the steps are as follows:

[0031] (1) Dissolve 0.5g of cationic polyacrylamide (CPAM) in 100mL of water to make a solution with a concentration of 5g / L.

[0032] (2) Add 0.5g of pulp fiber to 100mL of the cationic polyacrylamide solution prepared in step (1) to make the pulp concentration 0.5%, stir for 30min, then filter with a Buchner funnel and wash with deionized water.

[0033] (3) Put the fiber carrier prepared in step (2) into 100 mL of pectinase solution with a concentration of 0.25 wt%, so that the slurry fiber concentration is 0.5 wt%, stir for 30 min, filter with a Buchner funnel, wash with deionized water, and refrigerate For standby, the fibrous carrier immobilized enzyme for treating papermaking white water is prepared.

Embodiment 2

[0035] A preparation method for immobilized enzymes for processing papermaking white water, the steps are as follows:

[0036] (1) Dissolve 0.6g of cationic polyacrylamide (CPAM) in 100mL of water to make a solution with a concentration of 6g / L.

[0037] (2) Add 0.5g of pulp fiber to 100mL of the cationic polyacrylamide solution prepared in step (1) to make the pulp concentration 0.5%, stir for 30min, then filter with a Buchner funnel and wash with deionized water.

[0038] (3) Put the fiber carrier prepared in step (2) into 100 mL of pectinase solution with a concentration of 0.25%, so that the slurry fiber concentration is 0.5%, stir for 60 min, filter with a Buchner funnel, wash with deionized water, and refrigerate for later use. A fibrous carrier-immobilized enzyme for treating papermaking white water is prepared.

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Abstract

The invention relates to a preparation method of an immobilized enzyme used for processing papermaking white water. The method comprises the following steps: (1) dissolving cationic polyacrylamide (CPAM) in water to prepare a solution; (2) adding pulp fibers in the prepared CPAM solution for adsorption to make the concentration of pulp to be 0.1-0.8%, performing stirring for 30-60 min, and performing filtration and washing; and (3) adding the prepared fiber carrier in a pectase solution with the concentration of 0.1-0.25% for adsorption, and performing filtration and washing. The prepared immobilized enzyme is high in enzymatic activity, the quantity demand of cations in white water can be reduced, and the cost for processing white water for an industry is reduced.

Description

[0001] Technical field: [0002] The invention relates to an immobilized enzyme for treating papermaking white water, in particular to a preparation method for the immobilized enzyme for treating papermaking white water. [0003] Background technique: [0004] With the shortage of global water resources and the deepening of pollution, industrial water reuse and zero discharge have become hot topics in the scientific and technological circles and business circles. The paper industry is one of the most important industries in the world. Although it is developing from a traditional industry to an environment-friendly one, it still faces many problems that restrict its development. One of the characteristics of the papermaking process is the consumption of a large amount of water. In 2015, the average water intake per ton of pulp and paper was 70m 3 , 30-50m with foreign countries 3 There is still a big difference in water intake. Therefore, how to recycle water resources has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/08C02F101/34C02F103/28
CPCC02F3/342C02F2101/34C02F2103/26C12N11/08
Inventor 乌日娜胡晨丽黄鹏飞胡东乐
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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