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Separating and extracting method for six kinds of amide alkaloid in stems and leaves of Sabia parviflora Wall. ex Roxb.

A kind of technology of extracting method of Xiaohua Qingfengteng, which can be applied in the field of biology and can solve the problems of difficulty in separation and purification, etc.

Inactive Publication Date: 2017-01-04
CHONGQING MEDICAL & PHARMA COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the small differences in the structure of the above six amides, it is difficult to separate and purify

Method used

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  • Separating and extracting method for six kinds of amide alkaloid in stems and leaves of Sabia parviflora Wall. ex Roxb.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The method for separating and extracting six kinds of amide alkaloids in Teng chrysanthemum, the specific steps are:

[0027] A: Take the dried leaves of Qingfengteng xiaohuahua, crush them, and ultrasonically extract them with methanol 3.95 times its mass, for 4 hours each time, at a temperature of 50°C;

[0028] B: Concentrate the extract obtained after methanol extraction to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated solution, stir evenly, and place it overnight at 7°C;

[0029] C: Suction filter the solution left overnight with a Buchner funnel, discard the precipitate, and concentrate the filtrate to alcohol-free;

[0030] D: Dilute the concentrated solution obtained in step C by adding purified water twice its volume, extract the diluted solution with an equal volume of ethyl acetate, and concentrate the extract to obtain an extract;

[0031] E: Add the extract obtained in step D to 1.58 times the mass of m...

Embodiment 2

[0039] The method for separating and extracting six kinds of amide alkaloids in Teng chrysanthemum, the specific steps are:

[0040] A: Take the dried leaves of Qingfengteng xiaohuahua, crush them, and ultrasonically extract them with methanol 3.95 times its mass, for 4 hours each time, at a temperature of 50°C;

[0041] B: Concentrate the extract obtained after methanol extraction to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated solution, stir evenly, and place it overnight at 7°C;

[0042] C: Suction filter the solution left overnight with a Buchner funnel, discard the precipitate, and concentrate the filtrate to alcohol-free;

[0043] D: Dilute the concentrated solution obtained in step C by adding purified water twice its volume, extract the diluted solution with an equal volume of ethyl acetate, and concentrate the extract to obtain an extract;

[0044] E: Add the extract obtained in step D to 1.58 times the mass of m...

Embodiment 3

[0052] The method for separating and extracting six kinds of amide alkaloids in Teng chrysanthemum, the specific steps are:

[0053] A: Take the dried leaves of Qingfengteng xiaohuahua, crush them, and ultrasonically extract them with methanol 3.95 times its mass, for 4 hours each time, at a temperature of 50°C;

[0054] B: Concentrate the extract obtained after methanol extraction to one-tenth of the total extraction volume, add an equal volume of purified water to the concentrated solution, stir evenly, and place it overnight at 7°C;

[0055] C: Suction filter the solution left overnight with a Buchner funnel, discard the precipitate, and concentrate the filtrate to alcohol-free;

[0056] D: Dilute the concentrated solution obtained in step C by adding purified water twice its volume, extract the diluted solution with an equal volume of ethyl acetate, and concentrate the extract to obtain an extract;

[0057] E: Add the extract obtained in step D to 1.58 times the mass of m...

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Abstract

The invention belongs to the field of biology and particularly relates to a separating and extracting method for six kinds of amide alkaloid in stems and leaves of Sabia parviflora Wall. ex Roxb.. The separating and extracting method specifically comprises a purifying method including the steps of alcohol extracting-water precipitating, solvent extraction and prepared thin layer chromatography separation. The separating and extracting method solves the problem that as the structures of six kinds of amide compounds in stems and leaves of Sabia parviflora Wall. ex Roxb. have small differences, separation and extraction difficulty is high; the kinds of amide compounds are successfully separated and purified. The compound N-cis-N-coumaroyl tyramine is a novel natural product obtained from the plant, and the other five kinds of compounds are obtained from the plant through separation for the first time.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for separating and extracting six kinds of amide alkaloids in Teng hua qingfeng. Background technique [0002] Xiaohua Qingfengteng is a woody vine or climbing green shrub of the Qingfeng rattan family, and its roots, stems, branches and leaves can be used as medicine. Branches and leaves used as medicine have a good effect on the treatment of viral hepatitis. The leaves of Xiaohua Qingfengteng contain the following 6 kinds of amide compounds with medicinal use and research value of traditional Chinese medicine (abbreviated as compounds 1, 2, 3, 4, 5, 6 respectively): [0003] [0004] Due to the small structural differences of the above six amides, it is difficult to separate and purify them. Contents of the invention [0005] The object of the present invention is to provide a method for separating and extracting six kinds of amide alkaloids in Teng hua qingfe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C235/34C07C231/24
CPCC07C231/24C07C235/34
Inventor 闫志慧朱仝飞
Owner CHONGQING MEDICAL & PHARMA COLLEGE