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Preparation method and application of an electroluminescent immunosensor based on double co-reactant amplified signal

A technology of signal amplification and luminescence immunity, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, instruments, etc., can solve the problems of unsuitable rapid detection, large sample consumption, complex pretreatment, etc. Enhanced electrochemiluminescence intensity, highly sensitive detection, and simplified preparation process

Inactive Publication Date: 2019-04-02
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have high sensitivity and selectivity, but the detection process requires expensive special instruments, complex pretreatment and cumbersome operations, and large sample consumption, which is not suitable for rapid detection

Method used

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  • Preparation method and application of an electroluminescent immunosensor based on double co-reactant amplified signal
  • Preparation method and application of an electroluminescent immunosensor based on double co-reactant amplified signal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 A preparation method of an electrochemiluminescent immunosensor based on double co-reactant amplified signals:

[0028] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished with polishing powder in turn, ultrasonically cleaned in ethanol and ultrapure water for 5 min, dried with nitrogen, and 6 μL of CdS / MoS 2 The nanocomposite dispersion was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded CdS / MoS 2 Get CdS / MoS 2 / GCE;

[0029] (2) Take 10 μL of 10 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4°C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / CdS / MoS 2 / GCE;

[0030] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 1% and drop-coat it on the surface of the electrode, incubate a...

Embodiment 2

[0032] Example 2 A preparation method of an electrochemiluminescent immunosensor based on double co-reactants enhancing the light intensity of semiconductor nanocomposites:

[0033] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3 The polishing powder polished the glassy carbon electrode (GCE) with a diameter of 4 mm in turn, ultrasonically cleaned it in ethanol and ultrapure water for 5 min, and dried it with nitrogen, and took 8 μL of CdS / MoS 2 The nanocomposite dispersion was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded CdS / MoS 2 Get CdS / MoS 2 / GCE;

[0034] (2) Take 10 μL of 10 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4°C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / CdS / MoS 2 / GCE;

[0035] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 2% and dro...

Embodiment 3

[0037] Example 3 A preparation method of an electrochemiluminescent immunosensor based on double co-reactants enhancing the light intensity of semiconductor nanocomposites:

[0038] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3 The polishing powder polished the glassy carbon electrode (GCE) with a diameter of 4 mm in turn, ultrasonically cleaned it in ethanol and ultrapure water for 5 minutes, and dried it with nitrogen, and took 10 μL of CdS / MoS 2 The nanocomposite dispersion was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded CdS / MoS 2 Get CdS / MoS 2 / GCE;

[0039] (2) Take 10 μL of 10 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4°C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / CdS / MoS 2 / GCE;

[0040] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 3% ...

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Abstract

The invention relates to the technical field of electrochemiluminescence immune sensors, and in particular relates to a preparation method and application of an immune sensor taking a cadmium sulfide and molybdenum disulfide nano compound (CdS / MoS2) as a luminescent material and a substrate material and taking potassium persulfate and hydrogen peroxide as a dual-co-reaction reagent for enhancing luminescent intensity. Semiconductor nano materials, namely CdS and MoS2, with similar band gaps are compounded, so that the electric conductivity and electron-hole separation efficiency can be increased; and K2S2O8 and H2O2, serving as co-reaction reagents, have synergistic effects, so that the luminescent intensity of the sensor can be increased, and the stability can be enhanced. Based on specific binding between antigen antibodies, the sensor is used for detecting procalcitonin (PCT); and according to the difference of PCT with different concentration to the obstruction degree of electron transfer, the sensor has different electrochemiluminescence intensity, and detection of PCT is realized.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence immunosensors, in particular to a cadmium sulfide and molybdenum disulfide nanocomposite (CdS / MoS 2 ) is a luminescent material and a base material, and the preparation method and application of an immunosensor using potassium persulfate and hydrogen peroxide as double co-reactants. CdS and MoS 2 Combining two semiconductor nanomaterials with similar band gaps can effectively improve the luminous intensity of the sensor and enhance the stability. Potassium persulfate and hydrogen peroxide are co-reactants at the same time, which can play a synergistic effect and increase the luminous intensity of the sensor. Based on the specific binding between antigen and antibody, the sensor is used to detect procalcitonin (PCT). According to different concentrations of PCT, the degree of hindrance to electron transfer is different, and then the intensity of electrochemiluminescence of the sens...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/327G01N33/53
CPCG01N21/76G01N27/3278G01N33/53
Inventor 花小霞周长利王月娇
Owner UNIV OF JINAN
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