Agaricus gennadii HDAC1 enzyme inhibition effective part and preparation method and application

A technology of effective parts and spore mushrooms, applied in the field of plant extracts, can solve problems such as research and application that have not yet been seen, and achieve the effects of convenient extraction, high extraction efficiency, and improved extraction efficiency.

Inactive Publication Date: 2017-01-18
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research and application on the inhibition of HDAC1 enzyme activity by Agarita spp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiments 1 to 3 are specific implementations of the active part and preparation method of the mushroom tortillasporium inhibiting HDAC1 enzyme of the present invention, wherein embodiment 1 is the best embodiment.

[0023] Example 1

[0024]The preparation method comprises the steps of: pulverizing the tortuosporus mushroom, ultrasonically extracting with an ethanol solution with a concentration of 75% to 85% by volume for 1 hour, then filtering to obtain an ultrasonic extraction solution and filter residue, and adding 75% to 85% by volume concentration to the filter residue. Reflux extraction with 85% ethanol solution 3 times, 0.5-1 hour each time, filter to obtain the reflux extract, combine the ultrasonic extraction and reflux extract, concentrate and dry to obtain the extract; add distilled water to disperse the extract to obtain the extract dispersion, Then adopt sherwood oil, ethyl acetate and n-butanol successively as solvent extraction extract dispersion liqu...

Embodiment 2

[0026] The preparation method comprises the following steps: pulverize the tortular spore mushroom, use an ethanol solution with a volume percentage concentration of 85% to 95% for ultrasonic extraction for 3 hours, and then filter to obtain an ultrasonic extraction liquid and filter residue, and add 85% to the volume percentage concentration of the filter residue to the filter residue. Reflux extraction with 95% ethanol solution 3 times, each time for 1-1.5 hours, filter to obtain reflux extract, combine ultrasonic extraction and reflux extract, concentrate and dry to obtain extract; add distilled water to disperse extract to obtain extract dispersion, Then adopt sherwood oil, ethyl acetate and n-butanol successively as solvent extraction extract dispersion liquid, after volatilization removes solvent, obtain the mushroom petroleum ether part, mushroom ethyl acetate part, mushroom n-butanol part; After extracting the paste dispersion, the remaining liquid is concentrated and d...

Embodiment 3

[0028] The preparation method comprises the following steps: pulverize the tortular spore mushroom, use an ethanol solution with a volume percentage concentration of 65% to 75% for ultrasonic extraction for 0.5 hours, then filter to obtain the ultrasonic extraction liquid and filter residue, and add the volume percentage concentration of 65% to 75% to the filter residue. % ethanol solution reflux extraction twice, each time for 1.5 to 2 hours, filter to obtain the reflux extract, combine the ultrasonic extraction and reflux extract, concentrate and dry to obtain the extract; add distilled water to the extract to obtain the extract dispersion, and then Using petroleum ether, ethyl acetate and n-butanol in sequence as solvents to extract the extract dispersion, volatilize and remove the solvent to obtain the petroleum ether part, the ethyl acetate part of the mushroom and the n-butanol part of the mushroom; After the dispersion liquid is extracted, the remaining liquid is concent...

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Abstract

The invention provides an agaricus gennadii HDAC1 enzyme inhibition effective part and a preparation method and application and belongs to the technical field of plant extracts. The effective part is selected from a mixture of one or more of an agaricus gennadii petroleum ether part, an agaricus gennadii ethyl acetate part, an agaricus gennadii n-butyl alcohol part and an agaricus gennadii aqueous phase extraction part according to any mass ratio. Agaricus gennadii is smashed, an ethyl alcohol solution with the volume percentage concentration being 65-95% is added for reflux extraction, and an obtained extracting solution is concentrated and dried to obtain an extract; the extract is added into distilled water to be dispersed, an extract dispersion solution is obtained and extracted by sequentially using petroleum ether, ethyl acetate and n-butyl alcohol as solvents, and the agaricus gennadii petroleum ether part, the agaricus gennadii ethyl acetate part and the agaricus gennadii n-butyl alcohol part are obtained after the solvents are removed through volatilization; the remaining extract dispersion solution obtained after extraction is concentrated and dried, and the agaricus gennadii aqueous phase extraction part is obtained. The agaricus gennadii HDAC1 enzyme inhibition effective part is applied to preparing HDAC1 enzyme inhibition medicine and can be used for preparing anti-tumor medicine.

Description

technical field [0001] The invention relates to an effective part of Agaricus tortuosporum for inhibiting HDAC1 enzyme, a preparation method and an application, and belongs to the technical field of plant extracts. Background technique [0002] Agaricus gennadii belongs to the genus Agaricus (Agaricus) of the family Agaricaceae, and is also known as "Agaricus stalk mushroom" [see: Yang Hui, Li Na. Survey of mushrooms [J]. Nature, 1996 (6): 48.]. The tortuospore mushroom is umbrella-shaped and has a strong mushroom fragrance. The gills are milky white when young, and then gradually turn pink to dark brown, weighing 58~350g individually, and the maximum can reach 2~5kg. In summer and autumn, it grows in the sandy land of shrubs and in the 20-70cm soil under the reeds by the lake. It can open umbrellas underground. It grows solitary, scattered or in clusters. It mostly grows in the western and southwestern regions of Xinjiang [see: Wang Junyan. Xinjiang Edible and medicinal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P35/00
CPCA61K36/07A61K2236/333A61K2236/39A61K2236/55
Inventor 林鹏程吴疆左明丽廖志明包婷雯丁玉
Owner QINGHAI UNIV FOR NATITIES
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