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A research method of anti-tumor active ingredients in unicorn lotus

An anti-tumor activity, the technology of Unicornis, applied in the direction of anti-tumor drugs, medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, etc. stage, and the development of new drugs for anti-tumor effects of unicornis, to achieve the effect of high retention rate and scientific research methods

Active Publication Date: 2021-02-26
LUNAN PHARMA GROUP CORPORATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned documents show that the anti-tumor activity of unicorn has been accepted by most researchers, but most of the applicable test drugs in the prior art literature are the extract of unicorn or unicorn, and its effective active ingredient The research and application are still in the development stage, it is difficult to provide a basis for the development of new drugs with anti-tumor effect of unicorn lotus

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  • A research method of anti-tumor active ingredients in unicorn lotus
  • A research method of anti-tumor active ingredients in unicorn lotus
  • A research method of anti-tumor active ingredients in unicorn lotus

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Embodiment

[0019]Example The research method of the anti-tumor active ingredients in the unicornis of the present invention

[0020]1. The present invention provides a research method of anti-tumor active ingredients in Unicornis, which includes the following steps:

[0021]1) Preparation of unicornis extract: Fresh unicornis slices, dried and crushed, and 10 times the volume of water is added to the unicornis and the extraction temperature is controlled to 85-95℃ for extraction. The extraction time is 2h. After three consecutive extractions, combine and collect the water extract; add 95% ethanol to the water extract to adjust the alcohol concentration to 30%. After centrifugation, the supernatant is concentrated under reduced pressure until the ethanol concentration is less than 0.5%, and the water extract is obtained. Extract

[0022]2) Separation and purification of cation exchange resin column: transfer the water extract of the unicornis into a 001×7 cation exchange resin column, load the sample an...

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Abstract

The invention relates to a method for researching antineoplastic active components in dysoma pleiantha, which belongs to the technical field of traditional Chinese medicine. For clearing the antineoplastic active effective component of dysoma pleiantha, the method employs ethanol precipitate and an ion exchange resin for separating and purifying a dysoma pleiantha water extraction solution, and the tumor inhibition activity of the extract product can be detected and tracked through a S180 tumor-bearing mice model. The research method is suitable for researching antineoplastic activity in dysoma pleiantha, and provides certain base for developing the traditional Chinese medicine new drugs of dysoma pleiantha.

Description

Technical field[0001]The present invention relates to a research method of anti-tumor active ingredients in the Chinese edulis, belonging to the technical field of traditional Chinese medicine research.Background technique[0002]Unicornis (Latin scientific name: Typhonium giganteum Engl.) is a perennial herbaceous plant belonging to the genus Araceae, and the tubers of the unicornis are used as medicine. Its main functions: dry dampness and resolve phlegm, dispel wind and stop spasm, detoxify and dispel stagnation. It has a strong stimulating effect on the skin and mucous membranes. It can expel wind and phlegm, dispel cold and dampness, suppress spasm, treat headaches, crooked mouth and eyes, hemiplegia, tetanus, bruises and strains, numbness of the limbs, apoplexy, tuberculosis, and snake and insect bites. In recent years, studies have shown that the decoction of the medicine has anti-tumor effects, and animal model studies have shown that unicornis can significantly inhibit tumor ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/29A61K36/8884A61K36/8888A61P35/00
Inventor 张贵民关永霞宁停波杜丙源李涛
Owner LUNAN PHARMA GROUP CORPORATION