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Bacteria and application thereof for adsorbing heavy metal cadmium and copper

A technology for adsorbing heavy metals and heavy metals, applied in the biological field, can solve problems such as high cost and secondary pollution

Active Publication Date: 2019-12-10
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods are relatively effective, but the cost is high, and secondary pollution is easy to occur

Method used

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  • Bacteria and application thereof for adsorbing heavy metal cadmium and copper
  • Bacteria and application thereof for adsorbing heavy metal cadmium and copper
  • Bacteria and application thereof for adsorbing heavy metal cadmium and copper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: This embodiment illustrates the medium used in the present invention

[0038] Separation medium: beef extract 8 g, peptone 8 g, NaCl 5 g, distilled water 1000 mL, pH 7.2, CdCl 2 (10mg / L), CuCl 2 (100mg / L).

[0039] Fermentation medium: beef extract 8 g, peptone 8 g, NaCl 5 g, distilled water 1000 mL, pH 7.2.

Embodiment 2

[0040]Example 2: Bacteria Bacillus megaterium Isolation, screening and identification of XJ06

[0041] Put 10 g of soil from heavy metal-contaminated areas into 90 mL containing 10 mg / L CdCl 2 and 100 mg / L CuCl 2 The enrichment culture was carried out in the liquid beef extract peptone, and after 24 h, the enrichment culture solution was taken for 10 -2 、10 -3 、10 -4 For gradient dilution, 0.1 mL of each was spread on separate medium plates containing heavy metals cadmium and copper, and each gradient was replicated 3 times, and cultured at 25°C for 3 days. After the colonies grow out, pick colonies of different shapes, sizes, colors, etc., and inoculate them on corresponding plates, until there are no foreign colonies, and obtain purified strains. Inoculate the purified strains on the peptone slope of beef extract, and place them at 4 °C. Store in the refrigerator for later use.

[0042] The XJ06 strain identified after culturing and screening was cultured at 25°C for...

Embodiment 3

[0044] Embodiment 3: mutagenesis, screening of bacterial strain XJ06

[0045] Take a slant covered with the starting strain, put it in a sterile Erlenmeyer flask filled with glass beads, shake it fully at 25°C and 160rpm for 30min to disperse the bacteria evenly, filter to make a suspension, and dilute to a concentration of 10 7 pieces / ml. Take 0.1ml of the diluted bacterial suspension and spread it evenly, air-dry it, put it into the ion implanter, and use 15KeV N + , the injection volume is 50~200*10 13 / cm 2 , the target chamber vacuum is 10 -3 Pa handles the starting strain.

[0046] The mutated cells were eluted with normal saline to make 10 7 200ul of the bacteria suspension per ml was spread on a high-concentration isolation medium containing different concentrations of heavy metal ions, and cultured at 25°C for 6-7 days. During this period, the colony morphology on the plate was observed, and the total number of colonies was counted, and the colonies with the hi...

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PUM

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Abstract

The present invention provides a kind of bacterium that adsorbs heavy metal cadmium, copper and application thereof, and the classification of described bacterium that adsorbs heavy metal cadmium, copper is named Bacillus megaterium ( Bacillus megaterium ) XJ06‑26, the preservation number in Guangdong Microbial Culture Collection Center is: GDMCC NO: 60094. The bacterial strain is obtained through isolation, screening, identification and mutagenesis. The bacterium of the invention can effectively tolerate and absorb heavy metal cadmium and copper, and can effectively reduce the content of exchangeable cadmium at the same time, and the bacterium can be used for the treatment of water and soil polluted by heavy metal cadmium and copper.

Description

technical field [0001] The invention belongs to the field of biotechnology or environmental treatment technology, and relates to the discovery and mutagenesis of microorganisms and the removal of heavy metals by using the microorganisms. Background technique [0002] With the development of industries such as dyes and electroplating, and people's unreasonable mining of metal deposits, more and more heavy metals have entered water bodies. Heavy metals have teratogenic, carcinogenic, and mutagenic effects. Because heavy metals are not easily degraded by organisms, and can be passed and enriched through the food chain, they can enter the human body through various channels and seriously endanger human health. In my country, cadmium and copper are two common heavy metals that pollute the ecological environment. [0003] Cadmium can be enriched in organisms and enter the human body through the food chain to cause chronic poisoning. The biological half-life of cadmium is 10-30 y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/11C02F101/20
CPCB09C1/10C02F3/34C02F2101/20C12N1/205C12R2001/11
Inventor 黄和俞亚东江凌刘婷婷
Owner NANJING TECH UNIV
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