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Heavy metal resisting actinomycete strain and application thereof

A technology of heavy metals and actinomycetes, applied in the biological field, can solve the problems of non-biodegradable, high cost, secondary pollution, etc.

Active Publication Date: 2016-08-17
NANJING BEI SHENG RONG ENERGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods mainly include foreign soil, flushing and complexation, electrochemical methods, etc., which have quick results, but are costly and prone to secondary pollution; chemical methods usually change the form of heavy metals by adding foreign substances, so that soluble ions form insoluble precipitates substances, such as chemical modifiers, precipitants, etc., but the added substances are basically not biodegradable and will cause secondary pollution
There are no reports of Lechevalieria tolerance and adsorption of heavy metals

Method used

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  • Heavy metal resisting actinomycete strain and application thereof
  • Heavy metal resisting actinomycete strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: This embodiment illustrates the medium used in the present invention

[0028] Separation medium: glucose 5g / L, acid hydrolyzed casein 2g / L, yeast extract 1g / L, beef extract 1g / L, agar powder 15g / L, lead, copper and other heavy metals (0-1800mg / L).

[0029] Fermentation medium: glucose 5g / L, acid hydrolyzed casein 2g / L, yeast extract 1g / L, beef extract 1g / L, agar powder 15g / L.

Embodiment 2

[0030] Embodiment 2: Isolation, screening, identification of actinomycetes Lechevalieria NJ07

[0031] Weigh 1g of the soil sample from the heavy metal polluted area and dissolve it in 100ml of sterile normal saline, then carry out gradient dilution, select 10 -2 、10 -3 、10 -4 The dilutions were spread on separate medium plates containing heavy metals, with 3 replicates for each gradient, and cultured at 25°C. After the colonies grow out, pick colonies of different shapes, sizes, colors, etc. to streak and inoculate on the corresponding plates, until there are no foreign colonies. The NJ07 strain identified after culture and screening was cultured at 25°C for 6-7 days, and the morphology was observed. The hyphae in the base were well-branched and broken, and the air filaments were white. Refer to "Bergey's Handbook of Systematic Bacteriology" to conduct morphological, physiological and biochemical measurements on the NJ07 strain.

[0032] Table 1. Main physiological and bi...

Embodiment 3

[0037] Embodiment 3: Mutation, screening of bacterial strain NJ07

[0038] Take a slant of actinomycetes NJ07 covered with spores, elute the spores with normal saline, put them in a sterile triangular flask filled with glass beads, shake fully at 25°C and 160rpm for 30min to disperse the spores evenly, filter, and prepare Sporulation suspension, diluted to a spore concentration of 10 7 pcs / ml, take 0.1ml of the diluted spore suspension and spread it evenly, air-dry it, put it into the ion implanter, use 15KeV N+ , injection volume is 50~200*10 13 / cm 2 , the target chamber vacuum is 10 -3 Pa handles the spores.

[0039] The mutagenized spores were eluted with saline to make 10 7 200ul of the spore suspension per ml was spread on a high-concentration isolation medium containing different concentrations of heavy metal ions, and cultured at 25°C for 6-7 days. During this period, the colony morphology on the plate was observed, and the total number of colonies was counted, an...

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Abstract

The invention provides a heavy metal resisting actinomycete strain and an application thereof. The heavy metal resisting actinomycete strain is named as Lechevalieria nanjingensis NJ07-19, and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC No:M 2015737. The strain is obtained through separation, screening, identification and mutagenesis. The actinomyce can tolerate and adsorb multiple heavy metals, and can be used to treat heavy metal polluted water and soil.

Description

technical field [0001] The invention belongs to the field of biotechnology or environmental treatment technology, and relates to the discovery and mutagenesis of microorganisms and the removal of heavy metals by using the microorganisms. Background technique [0002] With the development of industry, people use more and more heavy metals (such as lead, copper, zinc, nickel, etc.), and the problems of heavy metal environmental pollution are also increasing. Heavy metals are not easy to be degraded by organisms, and will be enriched through the food chain, and finally enter the human body through various channels, endangering human health. [0003] Lead, lead is a heavy metal element that is highly toxic in pollutants and has no physiological function on the body and is mainly neurotoxic. Studies have shown that the safe concentration range of lead in the human body is 0.3-1mg. When the daily intake of lead exceeds this range, lead can accumulate in the blood, leading to anem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/04C02F101/20
CPCB09C1/10C02F3/34C02F2101/20C12N1/205C12R2001/04
Inventor 张志平
Owner NANJING BEI SHENG RONG ENERGY TECH CO LTD
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