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A nucleic acid aptamer for detection of carp spring viremia virus and its application

A technology of carp spring viremia and nucleic acid aptamer, which is applied in the field of carp spring viremia virus detection, can solve the problems of SVCV nucleic acid aptamer research and reports, and achieve low cost, simple and convenient use, and stability Good results

Active Publication Date: 2020-06-23
SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nucleic acid aptamers are relatively cutting-edge technologies, and there are still relatively few studies on aquatic animal viruses, and there are no studies and reports on SVCV nucleic acid aptamers or related reagents

Method used

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  • A nucleic acid aptamer for detection of carp spring viremia virus and its application
  • A nucleic acid aptamer for detection of carp spring viremia virus and its application
  • A nucleic acid aptamer for detection of carp spring viremia virus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] 1. Materials and methods

[0048] 1.1 Strains and cell lines

[0049] The SVCV A-1 strain used in this example was isolated and preserved by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau. The carp epithelioma cell line (EPC for short) is preserved by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau.

[0050] 1.2 Design and synthesis of aptamer library

[0051] The aptamer library in this example has the general formula shown in formula 2,

[0052] Formula 2: 5'-P 1 -N'-P 2 -3'

[0053] Among them, P 1 and P 2 They are the sequences shown in Seq ID No.1 and Seq ID No.2 respectively, and N' is a random sequence of 20-50 nt. P 1 and P 2 It is a sequence that specifically binds carp spring viremia virus, and N' is a random sequence generated by a synthetic company. In this example, an aptamer library with N' of 40 nt wa...

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Abstract

The invention discloses a nucleic acid aptamer for detecting spring viremia of carp virus and application thereof. The nucleic acid aptamer for detecting spring viremia of carp virus comprises one or two DNA fragments of a general formula as shown in a formula I; the formula I is 5'-P1-N-P2-3', wherein P1 and P2 are sequences as shown in Seq ID No. 1 and Seq ID No. 2 respectively, and N is a sequence as shown in Seq ID No. 3 or Seq ID No. 4. The nucleic acid aptamer for detecting spring viremia of carp virus can specifically and sensitively detect spring viremia of carp virus, and a novel approach and scheme are provided for detection of spring viremia of carp virus. The nucleic acid aptamer has higher practicality, and compared with enzyme-linked immunosorbent assay, the nucleic acid aptamer is low in cost and good in stability and simpler and more convenient to use.

Description

technical field [0001] The present application relates to the field of detection of carp spring viremia virus, in particular to a nucleic acid aptamer for detection of carp spring viremia virus and its application. Background technique [0002] Spring viremia of carp (Spring viremia of carp) is a disease listed by the World Organization for Animal Health (OIE) with a high fatality rate. The pathogen of carp spring viremia is Spring Viremia of carp virus (SVCV for short), which belongs to Rhabdoviridae. SVCV virus particles are bullet-shaped, 90-180nm long, 60-90nm wide, and the genome length is 11019nt. [0003] At present, the detection of SVCV mainly includes nucleic acid detection and ELISA. For example, "The establishment and application of digital RT-PCR detection method for carp spring viremia" published by Wu Bin et al., "China Animal Quarantine", 2015(7): 72 -76; "Establishment of SYBR Green I Fluorescent Quantitative RT-PCR Method for Carp Spring Viremia Virus" pu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115C12N15/10G01N33/569
CPCC12N15/1048C12N15/115C12N2310/16G01N33/56983C12N2330/31
Inventor 于力王津津郑晓聪贾鹏何俊强刘荭史秀杰兰文升杨锦舜
Owner SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT