Transgenic crop risk assessment method based on mesofauna multi-generation observation

A soil animal and risk assessment technology, applied in animal husbandry and other fields, can solve problems such as unconsidered response

Active Publication Date: 2017-01-25
JILIN ACAD OF AGRI SCI
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the environmental risk assessment of transgenic crop litter was mainly based on its impact on the survival rate and reproduction rate of soil animals in a single generation; Therefore, it is necessary to pay attention to the response of soil animals to transgenic crop litter for multiple generations; on the other hand, the survival rate and reproductive rate of soil animals are used as indicators, which only reflect the response of soil animal populations to transgenic crops. Responses of soil animals to GM crops are not considered

Method used

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  • Transgenic crop risk assessment method based on mesofauna multi-generation observation
  • Transgenic crop risk assessment method based on mesofauna multi-generation observation
  • Transgenic crop risk assessment method based on mesofauna multi-generation observation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Risk assessment method for transgenic crops based on multi-generational observations of mesozoic animals

[0027] Include the following steps:

[0028] 1. Preparation of soil animal medium: mix gypsum powder and activated carbon medium at a volume ratio of 9:5, and mix thoroughly with water at a volume ratio of 1:1. Put it in a petri dish for about 2 cm, shake After flattening, place it to dry and serve as the culture medium of the experimental group. At the same time, a control group was set up, and the culture medium of the control group was prepared by mixing gypsum powder and activated carbon at a volume ratio of 9:1, and the resulting mixed powder was mixed with sterile water in equal volumes.

[0029] 2. Litter treatment of transgenic corn Bt-799 and control Zheng 58: At the later stage of crop maturity, take 1000g of litter from transgenic and control crops, remove the petiole, cut into small pieces of length × width = 1cm × 1cm, and crush them with a ...

Embodiment 2

[0049] Example 2 Risk assessment method for transgenic crops based on multi-generational observations of mesozoic animals

[0050] Include the following steps:

[0051] 1. Preparation of soil animal medium: mix gypsum powder and activated carbon medium at a volume ratio of 9:5, and mix thoroughly with water at a volume ratio of 1:1. Put it in a petri dish for about 2 cm, shake After flattening, place it to dry and serve as the culture medium of the experimental group. At the same time, a control group was set up, and the culture medium of the control group was prepared by mixing gypsum powder and activated carbon at a volume ratio of 9:1, and the resulting mixed powder was mixed with sterile water in equal volumes.

[0052] 2. Treatment of transgenic corn IE09S034 and control 31 litter: In the later stage of crop maturity, take 1000 g of transgenic and control litter, remove the petioles, cut into small pieces of length × width = 1 cm × 1 cm, and crush them with a plant tissu...

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Abstract

The invention provides a transgenic crop risk assessment method based on mesofauna multi-generation observation. According to the method, mesofauna is raised in a culture medium with transgenic crop litter, survival rates, reproductive rates and body lengths of different generations of the mesofauna are counted, functional enzyme of the mesofauna is detected, and the influence of transgenic crops on continuous multi-generation mesofauna population fitness is comprehensively assessed. By improving a mesofauna culture medium formula, the culture medium is more adaptable to multi-generation continuous culture of the mesofauna by the transgenic crops. Besides, by monitoring response of acetylcholine esterase, superoxide dismutase, catalase and the like in the functional enzyme of the mesofauna to the transgenic crop litter, response of the mesofauna to the transgenic crops is comprehensively reflected according to parameters such as death rates and the survival rates of the mesofauna.

Description

technical field [0001] The invention belongs to the field of agricultural production, and in particular relates to a risk assessment method for transgenic crops based on multi-generational observation of medium-sized soil animals. Background technique [0002] Medium-sized soil animals refer to animals with a body width of 0.2mm-2mm that live in the soil for their whole life or part of their life history. Mesofauna are closely related to soil microorganisms and macrofauna, and play an important role in decomposing plant litter, improving soil fertility, and material circulation and energy flow in underground ecosystems. In the past, the environmental risk assessment of transgenic crop litter was mainly based on its impact on the survival rate and reproduction rate of soil animals in a single generation; Therefore, it is necessary to pay attention to the response of soil animals to transgenic crop litter for multiple generations; on the other hand, the survival rate and repr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 宋新元王柏凤常亮王大铭周琳尹俊奇武凤慈姜志磊
Owner JILIN ACAD OF AGRI SCI
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