Embossing method for bacterium sampling

An embossing method and bacterial technology, applied in the direction of biological material sampling method, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome and complicated procedures, heavy workload, and inaccurate sampling results, and achieve Accurate analysis results, improved work efficiency, and high promotional value

Inactive Publication Date: 2017-01-25
SHINVA MEDICAL INSTR CO LTD
7 Cites 2 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0004] The workload of this process is heavy, which is not conducive to improving the enthusiasm of medical staff to participate, and indirectly reduces the supervision of hand hygiene and environmen...
View more

Abstract

The invention relates to bacterium sampling method, and in particular to an embossing method for bacterium sampling. The embossing method for bacterium sampling comprises the following steps: firstly, preparing a neutralization liquid, mixing the neutralization liquid with nutrient agar, and sterilizing so as to obtain a mixed liquid; pouring the mixed liquid into a culture dish, cooling, and performing bacterium sampling by using embossing method, culturing, and calculating the clump count. The embossing method provided by the invention is convenient, simple and rapid, is capable of effectively improving the working efficiency, can be not only used for evaluating hand sterilization effects of hospitals, but also used for sampling bacteria on surfaces of articles, and has very high popularization values; when culture dish sampling is implemented by using the embossing method, a culture medium can sufficiently contact a hand or an environment surface, the culture counting process is sealed, no pollution can be caused, and analysis results can be relatively accurate.

Application Domain

Microbiological testing/measurementBiological material testing procedures

Technology Topic

Culture mediumsPollution +2

Image

  • Embossing method for bacterium sampling
  • Embossing method for bacterium sampling

Examples

  • Experimental program(2)

Example Embodiment

[0033] Example 1
[0034] The described imprinting method for hand bacterial sampling includes the following steps:
[0035] (1) To prepare neutralization solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add sodium hydroxide solution to adjust pH to 7.3 to obtain neutralization solution.
[0036] In the neutralization solution, the percentages of each component in the mass of water are: soybean lecithin 0.3%, Tween 80 0.3%, peptone 1%, sodium chloride 0.5%, sodium thiosulfate 0.1%, glycine 0.3%.
[0037] (2) After uniformly mixing the neutralized solution and the dissolved nutrient agar, it is placed in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.
[0038] (3) After cooling the mixed solution to 42°C, pour it into a culture dish, cool it for use, and the cooled mixed solution becomes a culture medium.
[0039] (4) Press the surface of the medium on the culture dish directly to the base of the palm to the root of the finger after 15 seconds, cultivate for 24 hours, and count according to the grid on the culture dish.
[0040] The amount of the mixture poured into the culture dish is 1mm higher than the annular platform.
[0041] The volume ratio of neutralization solution to dissolved nutrient agar was 1:4.

Example Embodiment

[0042] Example 2
[0043] The described imprinting method for bacterial sampling on the surface of objects includes the following steps:
[0044] (1) To prepare neutralization solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add dilute hydrochloric acid solution to adjust pH to 7.2 to obtain neutralization solution.
[0045] In the neutralization solution, the percentages of each component in the mass of water are: soybean lecithin 0.2%, Tween 80 0.4%, peptone 0.8%, sodium chloride 0.6%, sodium thiosulfate 0.15%, glycine 0.4%.
[0046] (2) After uniformly mixing the neutralized solution and the dissolved nutrient agar, it is placed in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.
[0047] (3) After cooling the mixed solution to 45°C, pour it into a culture dish, cool it for use, and the cooled mixed solution becomes a culture medium.
[0048] (4) During detection, press the surface of the culture dish on the object to be inspected for 20 seconds, incubate for 48 hours, and count according to the grid on the culture dish.
[0049] The amount of the mixture poured into the culture dish is 2mm higher than the annular platform.
[0050] The volume ratio of neutralization solution to dissolved nutrient agar was 1:5.

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Similar technology patents

Detection system for spectral resolution of Rowland grating spectrometer

ActiveCN109724696Agood stabilityThe analysis result is accurate
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI

Rod piece load holding testing device

InactiveCN104849145AThe analysis result is accurate
Owner:GUIZHOU TRANSPORTATION PLANNING SURVEY & DESIGN ACADEME

Intelligent financial data analysis system and method

PendingCN113077115AThe analysis result is accurateHigh degree of intelligence
Owner:上海慧博网络信息有限公司

Energy-saving evaluation mode for coal-fired grate-fired industrial boiler based on numerical simulation

PendingCN111914399AAccurately evaluate energy saving performanceThe analysis result is accurate
Owner:CHINA ELECTRONICS STANDARDIZATION INST +1

Novel infrared carbon and sulfur analyzer

InactiveCN109406437Aconstant internal and external temperatureThe analysis result is accurate
Owner:南京奥科分析仪器有限公司

Classification and recommendation of technical efficacy words

  • The analysis result is accurate
  • Improve work efficiency

Mechanical fault diagnosis method based on parameter adaptive VMD

ActiveCN108692936AStrong signal detection abilityThe analysis result is accurate
Owner:SICHUAN UNIV

Method for measuring TSNAs (tobacco-specific nitrosamines) in electronic cigarette smoke

ActiveCN104237431AThe analysis result is accurategood repeatability
Owner:CHINA TOBACCO YUNNAN IND

Intelligent voice recognition interactive lighting method and system based on emotion judgment

PendingCN108882454AEnergy-saving and comfortable extraction featuresThe analysis result is accurate
Owner:FOSHAN UNIVERSITY

Intelligent antitheft system and method

InactiveCN105205966AThe analysis result is accurate
Owner:PHICOMM (SHANGHAI) CO LTD

Quantitative method for fish diversity based on eDNA and sampling and filtering device thereof

ActiveCN109593829Aavoid cross contaminationThe analysis result is accurate
Owner:BEIJING NORMAL UNIVERSITY

Cooperation service platform facing different source data

InactiveCN101174957AImprove management level and qualityImprove work efficiency
Owner:NANJING UNIV OF FINANCE & ECONOMICS

Screw drilling tool, vertical drilling tool testing method and well inclination simulation testing equipment

InactiveCN111594144AReduced height requirementsImprove work efficiency
Owner:DEZHOU UNITED GASOLINEEUM MACHINERY

Method for intelligent automatic identification of transmission circuit parts

Owner:INFORMATION COMM COMPANY STATE GRID SHANDONG ELECTRIC POWER +2

Keyboard spill-proofing mechanism

ActiveUS7030330B2minimize manufacturing costimprove work efficiency
Owner:LITE ON SINGAPORE PTE LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products