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Embossing method for bacterium sampling

An embossing method and bacterial technology, applied in the direction of biological material sampling method, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome and complicated procedures, heavy workload, and inaccurate sampling results, and achieve Accurate analysis results, improved work efficiency, and high promotional value

Inactive Publication Date: 2017-01-25
SHINVA MEDICAL INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The workload of this process is heavy, which is not conducive to improving the enthusiasm of medical staff to participate, and indirectly reduces the supervision of hand hygiene and environmental hygiene
At the same time, the procedures are cumbersome and complicated, and it is impossible to ensure that every step is a sterile operation, which may easily lead to inaccurate sampling results.

Method used

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  • Embossing method for bacterium sampling
  • Embossing method for bacterium sampling

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The described imprinting method for hand bacterial sampling comprises the following steps:

[0035] (1) To prepare a neutralizing solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add sodium hydroxide solution to adjust the pH to 7.3 to obtain a neutralizing solution.

[0036] In the neutralizing solution, the percentages of each component in the mass of water are: 0.3% soybean lecithin, 0.3% Tween 80, 1% peptone, 0.5% sodium chloride, 0.1% sodium thiosulfate, and 0.3% glycine.

[0037] (2) After mixing the neutralizing solution and the dissolved nutrient agar evenly, put it in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.

[0038] (3) Cool the mixed solution to 42° C., pour it into a culture dish, and cool it for later use. The cooled mixed solution becomes a culture medium.

[0039] (4) Press the medium surface on the culture dish directly from the base of the palm to t...

Embodiment 2

[0043] The described imprinting method for sampling bacteria on the surface of an object comprises the following steps:

[0044] (1) To prepare a neutralizing solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add dilute hydrochloric acid solution to adjust the pH to 7.2 to obtain a neutralizing solution.

[0045] In the neutralizing solution, the percentages of each component in the mass of water are: soybean lecithin 0.2%, Tween 80 0.4%, peptone 0.8%, sodium chloride 0.6%, sodium thiosulfate 0.15%, glycine 0.4%.

[0046] (2) After mixing the neutralizing solution and the dissolved nutrient agar evenly, put it in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.

[0047] (3) Cool the mixed solution to 45°C, pour it into a culture dish, and cool it for later use. The cooled mixed solution becomes a culture medium.

[0048] (4) During detection, press the surface of the culture dish o...

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Abstract

The invention relates to bacterium sampling method, and in particular to an embossing method for bacterium sampling. The embossing method for bacterium sampling comprises the following steps: firstly, preparing a neutralization liquid, mixing the neutralization liquid with nutrient agar, and sterilizing so as to obtain a mixed liquid; pouring the mixed liquid into a culture dish, cooling, and performing bacterium sampling by using embossing method, culturing, and calculating the clump count. The embossing method provided by the invention is convenient, simple and rapid, is capable of effectively improving the working efficiency, can be not only used for evaluating hand sterilization effects of hospitals, but also used for sampling bacteria on surfaces of articles, and has very high popularization values; when culture dish sampling is implemented by using the embossing method, a culture medium can sufficiently contact a hand or an environment surface, the culture counting process is sealed, no pollution can be caused, and analysis results can be relatively accurate.

Description

technical field [0001] The invention relates to a method for sampling bacteria, in particular to an imprinting method for sampling bacteria. Background technique [0002] The number of bacteria on the surface of objects and on the hands of medical staff and the evaluation method are the routine detection methods of the country in accordance with the "Hospital Disinfection and Sanitation Standards". The staff of each department needs to carry out sampling and testing. There are many sampling points, high frequency, complicated sampling process, and many factors affecting the sampling results. [0003] According to the requirements of hand hygiene standards for medical personnel, after disinfection, the five fingers of the subject should be put together, and a cotton swab soaked in a sterile eluent containing the corresponding neutralizer should be used to go back and forth on the curved surface of both fingers from the heel to the fingertip Wipe 2 times, one hand rubs the ar...

Claims

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Application Information

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IPC IPC(8): C12Q1/22C12M1/26
CPCC12M23/10C12M33/00C12Q1/22
Inventor 庞元金李泽敬王久儒
Owner SHINVA MEDICAL INSTR CO LTD
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