Embossing method for bacterium sampling

An embossing method and bacterial technology, applied in the direction of biological material sampling method, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome and complicated procedures, heavy workload, and inaccurate sampling results, and achieve Accurate analysis results, improved work efficiency, and high promotional value

Inactive Publication Date: 2017-01-25

SHINVA MEDICAL INSTR CO LTD

7 Cites 2 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0004] The workload of this process is heavy, which is not conducive to improving the enthusiasm of medical staff to participate, and indirectly reduces the supervision of hand hygiene and environmen...

Abstract

The invention relates to bacterium sampling method, and in particular to an embossing method for bacterium sampling. The embossing method for bacterium sampling comprises the following steps: firstly, preparing a neutralization liquid, mixing the neutralization liquid with nutrient agar, and sterilizing so as to obtain a mixed liquid; pouring the mixed liquid into a culture dish, cooling, and performing bacterium sampling by using embossing method, culturing, and calculating the clump count. The embossing method provided by the invention is convenient, simple and rapid, is capable of effectively improving the working efficiency, can be not only used for evaluating hand sterilization effects of hospitals, but also used for sampling bacteria on surfaces of articles, and has very high popularization values; when culture dish sampling is implemented by using the embossing method, a culture medium can sufficiently contact a hand or an environment surface, the culture counting process is sealed, no pollution can be caused, and analysis results can be relatively accurate.

Application Domain

Microbiological testing/measurementBiological material testing procedures

Technology Topic

Culture mediumsPollution +2

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Examples

Experimental program(2)

Example Embodiment

[0033] Example 1

[0034] The described imprinting method for hand bacterial sampling includes the following steps:

[0035] (1) To prepare neutralization solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add sodium hydroxide solution to adjust pH to 7.3 to obtain neutralization solution.

[0036] In the neutralization solution, the percentages of each component in the mass of water are: soybean lecithin 0.3%, Tween 80 0.3%, peptone 1%, sodium chloride 0.5%, sodium thiosulfate 0.1%, glycine 0.3%.

[0037] (2) After uniformly mixing the neutralized solution and the dissolved nutrient agar, it is placed in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.

[0038] (3) After cooling the mixed solution to 42°C, pour it into a culture dish, cool it for use, and the cooled mixed solution becomes a culture medium.

[0039] (4) Press the surface of the medium on the culture dish directly to the base of the palm to the root of the finger after 15 seconds, cultivate for 24 hours, and count according to the grid on the culture dish.

[0040] The amount of the mixture poured into the culture dish is 1mm higher than the annular platform.

[0041] The volume ratio of neutralization solution to dissolved nutrient agar was 1:4.

Example Embodiment

[0042] Example 2

[0043] The described imprinting method for bacterial sampling on the surface of objects includes the following steps:

[0044] (1) To prepare neutralization solution, add soybean lecithin, Tween 80, peptone, sodium chloride, sodium thiosulfate and glycine into water, then add dilute hydrochloric acid solution to adjust pH to 7.2 to obtain neutralization solution.

[0045] In the neutralization solution, the percentages of each component in the mass of water are: soybean lecithin 0.2%, Tween 80 0.4%, peptone 0.8%, sodium chloride 0.6%, sodium thiosulfate 0.15%, glycine 0.4%.

[0046] (2) After uniformly mixing the neutralized solution and the dissolved nutrient agar, it is placed in a high-pressure steam sterilizer for sterilization to obtain a mixed solution.

[0047] (3) After cooling the mixed solution to 45°C, pour it into a culture dish, cool it for use, and the cooled mixed solution becomes a culture medium.

[0048] (4) During detection, press the surface of the culture dish on the object to be inspected for 20 seconds, incubate for 48 hours, and count according to the grid on the culture dish.

[0049] The amount of the mixture poured into the culture dish is 2mm higher than the annular platform.

[0050] The volume ratio of neutralization solution to dissolved nutrient agar was 1:5.

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Classification and recommendation of technical efficacy words

The analysis result is accurate
Improve work efficiency

Embossing method for bacterium sampling

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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