A fully automatic intelligent cell culture device and its control method

Abstract

The invention relates to the field of cell culture technologies, and in particular to a full-automatic intelligent cell culture device and a control method thereof. The cell culture device comprises a controller, a first regulating mechanism, a second regulating mechanism, a third regulating mechanism, a culture shelf, a first control door, a second control door, a first pipetting mechanism, a second pipetting mechanism, a waste liquid collection mechanism, a bottle opening / closing mechanism and a liquid adding mechanism; and at least three cavities, which include a keeping cavity, a transition cavity and a pre-heating cavity, are arranged in a storage cavity. The culture device provided by the invention is convenient, rapid and precise in liquid replacing and has no influence on cell growth; and moreover, the culture device is convenient for pre-heating and is relatively high in pre-heating speed.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a fully automatic intelligent cell culture device and a control method thereof. Background technique [0002] In the field of cell biology research and life science research, cell culture is the most basic link. Cell culture is a process of cultivating cells in an artificial environment, using nutrients, etc., to multiply and increase their value, and to use them for "various purposes". [0003] In the prior art, cell culture needs to be divided into two environments, one is the storage environment of the culture solution at low temperature, and the culture solution mainly includes the culture medium, digestive fluid and buffer solution, and the other is the cell culture environment at a suitable temperature, both of which are in the prior art It is set separately. When the culture medium needs to be replaced or digested and passaged, the operator needs to manually take out...

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Problems solved by technology

[0003] In the prior art, cell culture needs to be divided into two environments, one is the storage environment of the culture medium at low temperature, and the culture medium mainly includes culture medium, digestive fluid and buffer solution, and the other is the cell culture environment at a suitable temperature, both of which are in the prior art It is set separately. When the culture medium needs to be replaced or digested and passaged, the operator needs to manually take out the culture medium bottle storing the culture medium from one of the culture environments, and take out the cell culture bottle from the other culture environment, and then the workers pass through. The tool is used to replace the culture solution, and finally the culture solution bottle and the cell culture bottle are put back into their respective culture environments. This method of operation has the following disadvantages: 1. Manual operation makes it difficult to accurately control the amount of culture solution; 2. 1. Manual operation requires removing the cell culture bottle from the culture environment, which may affect the growth of cells and have a certain impact on the accuracy of the experiment; 3. Manual operation is used, so the replacement efficiency of the culture medium is low and error-prone; 4. , Manual operation is adopted, and the time for replacing the culture medium cannot be selected arbitrarily, and the replacement can only be performed when an operator is needed

[0004] And the culture solution of the prior art generally all uses air as the medium to carry out temperature adjustment, so when the culture solution needs to be preheated, the culture solution needs to be taken out and placed in a preheating environment, which is not very convenient, and because it uses air as the medium, so the warm-up speed is slower

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