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Periplaneta americana medicinal material fingerprint quality determination method

A kind of American cockroach, fingerprint technology, applied in the direction of measurement device, material separation, analysis of materials, etc., can solve the problems of sample detection error, cumbersome preparation method, time-consuming and other problems, and achieve good separation, repeatability and stability. Effect

Active Publication Date: 2017-02-01
SICHUAN GOODDOCTOR PANXI PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent No. CN101320027 discloses a method for measuring the quality of fingerprints of Periplaneta americana medicinal materials and their extracts. In this method, the preparation method of the test solution of Periplaneta americana medicinal materials is too cumbersome and time-consuming, which will inevitably lead to errors in sample detection. , reducing the accuracy of analysis, and the amount of information in the established fingerprints is small, it is difficult to obtain a more comprehensive characterization of the medicinal materials of Periplaneta americana with complex components

Method used

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  • Periplaneta americana medicinal material fingerprint quality determination method
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  • Periplaneta americana medicinal material fingerprint quality determination method

Examples

Experimental program
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Effect test

experiment example 1

[0019] (1) Instruments and reagents

[0020] LC-6AD High Performance Liquid Chromatograph (Shimadzu Corporation), KQ5200 Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), RE-52 Rotary Evaporator (Shanghai Yarong Biochemical Instrument Factory).

[0021] Absolute ethanol is analytically pure, methanol is chromatographically pure, glacial acetic acid is analytically pure, and self-made ultrapure water.

[0022] Samples of Periplaneta americana were provided by Sichuan Good Doctor Panxi Pharmaceutical Co., Ltd.

[0023] (2) Investigation of the extraction conditions of Periplaneta americana ethanol extract

[0024] a. Sample pretreatment

[0025] Get the dry sample of the American cockroach body and grind it into powder with a pulverizer, and pass through a 40-60 mesh sieve to remove larger particles. Remove the contained lipids before soaking the sample to reduce its influence on the test. Before being treated with petroleum ether, the sample to be tested was wra...

Embodiment 2

[0093] (a) Sample pretreatment: take the dry sample of Periplaneta americana and crush it and pass through a 40-mesh sieve, wrap the obtained sample in a degreased filter paper bag, put it into an extraction tube, add petroleum ether to the extraction bottle, and use the Soxhlet extraction method , remove the grease in the Periplaneta americana sample, pour out the dregs of the medicine, and evaporate the dregs of the medicine;

[0094] (b) Preparation of the test solution: accurately weigh 1 part by weight of the drug dregs, place it in a conical flask, add 30 parts by volume of 40% ethanol, perform ultrasonic treatment at 50° C. for 1 h, let it cool, and distill the ethanol out under reduced pressure. Afterwards, transfer to a 25-volume measuring bottle, add water to constant volume and shake evenly, place the solution in a centrifuge at 5000 rpm for 5 minutes, take the supernatant and filter it through a 0.45 μm microporous membrane, and take the filtrate for subsequent use;...

Embodiment 3

[0100] (a) Sample pretreatment: take the dry sample of Periplaneta americana and crush it and pass through a 60-mesh sieve, wrap the obtained sample in a degreased filter paper bag, put it into an extraction tube, add petroleum ether to the extraction bottle, and use the Soxhlet extraction method , remove the grease in the Periplaneta americana sample, pour out the dregs of the medicine, and evaporate the dregs of the medicine;

[0101] (b) Preparation of the test solution: Accurately weigh 5 parts by weight of the medicinal residue, place it in a conical flask, add 150 parts by volume of 80% ethanol, ultrasonically treat it at 50°C for 3 hours, let it cool, and distill out the ethanol under reduced pressure , transfer the dregs to a 100-volume bottle, add water to constant volume and shake well, place the solution in a centrifuge at 5000 rpm for 5 minutes, take the supernatant and filter it through a 0.45 μm microporous membrane, and take the filtrate for subsequent use;

[0...

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Abstract

The invention relates to a periplaneta americana medicinal material fingerprint quality determination method. The fingerprint obtained by the periplaneta americana medicinal material fingerprint quality determination method has a good chromatographic peak resolution degree. The determination method has reasonable detection time and good repeatability and stability. Compared with the existing reported periplaneta americana medicinal material fingerprint technology, the method improves a periplaneta americana medicinal material quality control level. The fingerprint technology effectively evaluates and controls periplaneta americana medicinal material quality, guarantees periplaneta americana extract quality stability under a certain preparation condition, further effectively controls quality of periplaneta americana extract-containing preparations such as Kangfuxin tablets, Kangfuxin capsules, a Kangfuxin liquid, Xinmailong, Ganlong capsules and the like.

Description

technical field [0001] The invention relates to a method for measuring the quality of traditional Chinese medicinal materials, in particular to a method for determining the quality of fingerprints of Periplaneta americana medicinal materials. Background technique [0002] The medicinal materials of Periplaneta americana are included in the 1996 edition of "Drug Standards of Yunnan Province". The medicinal materials of Periplaneta americana mainly contain neuropeptides, nucleosides, amino acids, polysaccharides and other components, and have been developed into various preparations, such as Kangfuxin Liquid, Ganlong Capsules, Xinmailong and Xiaozhengyigan Tablets. Patent No. CN101320027 discloses a method for measuring the quality of fingerprints of Periplaneta americana medicinal materials and their extracts. In this method, the preparation method of the test solution of Periplaneta americana medicinal materials is too cumbersome and time-consuming, which will inevitably lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘越飞耿福能沈咏梅
Owner SICHUAN GOODDOCTOR PANXI PHARMA
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