Method for quickly making models of diabetic mice

A technology of mouse model and diabetes, which is applied in the fields of pharmaceutical formula and drug delivery, can solve the problems of low molding rate, difficult to control dosage, and low molding rate of mice

Inactive Publication Date: 2017-02-08
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used experimental induction modeling method takes a long time, and the modeling rate is not high; it is difficult to control the dosage of alloxan or streptozotoci

Method used

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  • Method for quickly making models of diabetic mice
  • Method for quickly making models of diabetic mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0007] 1 experimental equipment

[0008] Blood glucose tester (China Sinocare Biosensing Co., Ltd., blood glucose test strips, batch number: 2610NK), precision electronic balance (Mettler-Toledo Instruments (Shanghai) Co., Ltd.), 1ml syringe (Guangxi Beilunhe Medical Industry Group) Ltd.) etc.

[0009] 2 Experimental animals and materials

[0010] Animals: male KM mice, clean grade, weighing 24±2g, provided by the Experimental Animal Center of Guangxi Medical University, license number: SCXK Gui 2014-0002.

[0011] Materials: Alloxan (US Sigma Company, batch number: BCBN9150V), normal saline, etc.

[0012] 3 Experimental methods

[0013] Purchase 60 male KM mice with a body weight of 24±2g. The temperature of the feeding environment is 26±3°C and the humidity is about 50-60%. hours (must be replaced with new bedding every time fasting and water, the same below), were randomly divided into 3 groups according to body weight: A group, B group, and C group, A group was 10 norm...

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Abstract

The invention discloses a method for quickly making models of diabetic mice. Male Kunming-strain mice are adopted, and are raised until body weights reach 30(+/-)2g before an experiment, and then the experiment is carried out. The method comprises the following steps of enabling the mice to fast without depriving water for 18 hours, then directly injecting tetraoxypyrimidine from caudal veins with the dosages of 35mg per kilogram of the body weights, measuring blood sugar by using a blood sugar testing instrument on the sixth day after the injection, electing the mice succeeded in modeling, and continuously giving conventional raising to the mice; on the same day when the blood sugar is measured, continuously enabling the mice which are not modeled to fast without depriving the water until 24 hours, then injecting the tetraoxypyrimidine to the mice which are not modeled from the caudal veins with the dosages of 70mg per kilogram of the body weights once again, measuring the blood sugar by using the blood sugar testing instrument on the sixth day after the injection, and electing the mice succeeded in the modeling. The blood sugar values of the mice succeeded in the modeling are between 16.0mmol/L to 28.0mmol/L; the method for quickly making the models of the diabetic mice can be used for a relevant animal experiment in which the diabetic mouse is used as the model. The success rate of establishment of the model of the method reaches 85.00 percent; no death occurs; the modeling time is short; the experiment is simple and convenient, and is easy to operate.

Description

technical field [0001] The invention relates to a method for rapidly producing a diabetic mouse model, in particular to a method for rapidly producing a diabetic mouse model by injecting alloxan in divided doses. Background technique [0002] At present, the commonly used diabetic animal models include experimentally induced and spontaneous diabetic mouse models. The experimental induction method is mainly to create models by injecting alloxan or streptozotocin. Nowadays, there are usually two methods for making models with alloxan or streptozotocin: the first one is to feed them with high-fat and high-sugar feed for about 2 months, and then inject the same dose of tetraoxine one or more times continuously. Pyrimidine or streptozotocin, the blood glucose value was measured by taking blood from the tail vein at the end, and judging whether the modeling was successful or not according to the blood glucose value; the second type did not feed with high-fat and high-sugar feed, ...

Claims

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Application Information

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IPC IPC(8): A61K31/513
CPCA61K31/513A61K9/0019
Inventor 夏中尚杜正彩邓家刚侯小涛郝二伟
Owner GUANGXI UNIV OF CHINESE MEDICINE
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