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Method for preparing and reproducing tobacco leaves with microorganisms

A technology of reconstituted tobacco leaves and microorganisms, which is applied in the field of preparing reconstituted tobacco leaves and using microorganisms to prepare reconstituted tobacco leaves, achieving the effects of simple operation, good social and economic benefits, and improved taste quality

Active Publication Date: 2017-02-15
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of Maillard reaction in tobacco production, especially in the extraction stage of reconstituted tobacco leaves, through microbial methods to promote Maillard reactions to produce some aromatic substances to improve the sensory quality of reconstituted tobacco leaves

Method used

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  • Method for preparing and reproducing tobacco leaves with microorganisms
  • Method for preparing and reproducing tobacco leaves with microorganisms
  • Method for preparing and reproducing tobacco leaves with microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for preparing reconstituted tobacco leaves using microorganisms, comprising the following steps:

[0030] (1) Put tobacco raw material and water into the fermenter according to the ratio of 1:6 in mass ratio, after leaching for 20min, add Bacillus cereus bacterial liquid according to 6% inoculum amount (tobacco raw material+water), bacterial liquid The OD value of 1.0, leaching, Maillard reaction occurs simultaneously during leaching, separates after leaching, obtains extract and insoluble matter;

[0031] The extraction conditions are: temperature 50°C, time 2h, stirring speed 150rpm, dissolved oxygen 20%;

[0032] The tobacco raw material is a mixture of tobacco stems, tobacco dust and low-grade tobacco leaves at a mass ratio of 5:3:2;

[0033] (2) Carry out vacuum rotary evaporation concentration to extract solution to obtain concentrated solution, the Baume degree of concentrated solution is 25 ° Bé; Carry out papermaking technology to copy insoluble matte...

Embodiment 2

[0036] A method for preparing reconstituted tobacco leaves using microorganisms, comprising the following steps:

[0037] (1) Tobacco raw material and water are added in the fermenter according to the ratio of 1:6 according to the mass ratio, after leaching for 20min, then add Bacillus cereus bacterium liquid according to the inoculation amount of 10%, the OD value of bacterium liquid is 1.0, Leaching, Maillard reaction occurs at the same time as leaching, and separation is carried out after leaching to obtain extract and insoluble matter;

[0038] The extraction conditions are: temperature 40°C, time 2h, stirring speed 150rpm, dissolved oxygen 20%;

[0039] The tobacco raw material is a mixture of tobacco stems, tobacco dust and low-grade tobacco leaves at a mass ratio of 5:3:2;

[0040] (2) Carry out vacuum rotary evaporation concentration to extract solution to obtain concentrated solution, the Baume degree of concentrated solution is 25 ° Bé; Carry out papermaking technol...

Embodiment 3

[0043] A method for preparing reconstituted tobacco leaves using microorganisms, comprising the following steps:

[0044] (1) Tobacco raw material and water are added in the fermenter according to the ratio of 1:6 according to the mass ratio, after leaching for 20min, then add Bacillus cereus bacterium liquid according to the inoculation amount of 8%, the OD value of bacterium liquid is 1.0, Leaching, Maillard reaction occurs at the same time as leaching, and separation is carried out after leaching to obtain extract and insoluble matter;

[0045] The extraction conditions are: temperature 35°C, time 3h, stirring speed 150rpm, dissolved oxygen 20%;

[0046] The tobacco raw material is a mixture of tobacco stems, tobacco dust and low-grade tobacco leaves at a mass ratio of 5:3:2;

[0047] (2) Carry out vacuum rotary evaporation concentration to extract solution to obtain concentrated solution, the Baume degree of concentrated solution is 25 ° Bé; Carry out papermaking technolo...

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Abstract

The invention discloses a method for preparing and reproducing tobacco leaves with microorganisms and belongs to the technical field of tobacco. The method comprises the steps of adding bacillus cereus into soaked tobacco raw materials to degrade protein in the soaked tobacco raw materials, and generating precursor-aroma substances by participating in a Maillard reaction. According to the method for preparing and reproducing tobacco leaves with microorganisms, during the process of extracting the tobacco raw materials, the bacillus cereus is added, the protein in the tobacco raw materials can be degraded to the utmost extent to generate polypeptide or free amino acid, meanwhile, soluble reducing sugar in the tobacco raw materials during the extracting process is dissolved in the extracted liquor, which gives rise to the Maillard reaction between the degraded product and the soluble reducing sugar, thus the precursor-aroma substances with heterocyclic compounds are generated. These precursor-aroma substances participates in complex chemical reactions to generate aromas through thermal cracking when cigarettes burns, thus good harmony and compatibility are generated between the reproduced tobacco leaves and the cigarettes and taste quality of the cigarettes is improved.

Description

technical field [0001] The invention relates to a method for preparing reconstituted tobacco leaves, in particular to a method for preparing reconstituted tobacco leaves by using microorganisms, and belongs to the technical field of tobacco. Background technique [0002] In the process of cigarette production in my country, a large amount of tobacco waste will be produced. For these waste materials, countries around the world mainly use the method of reconstituted tobacco leaves to re-use the waste materials. The production methods of reconstituted tobacco leaves mainly include roller pressing, thick pulp method and papermaking method. At present, my country mainly adopts papermaking method to deal with tobacco leaf waste in the production process. The papermaking method is to make tobacco materials such as tobacco powder, tobacco stems, cigarette sticks, tobacco sticks and some low-grade tobacco leaves from rebaking in the tobacco manufacturing process and some tobacco was...

Claims

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Application Information

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IPC IPC(8): A24B3/14
CPCA24B3/14
Inventor 杨雪鹏叶建斌毛多斌杨峰马科闫记聂晶晶汪芳芳
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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