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Combined extraction method for rutin and polysaccharide in buckwheat seeds

A technology of combined extraction and tartary buckwheat seeds, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of less research on the comprehensive utilization of tartary buckwheat polysaccharides and flavonoids, and achieve abundant and cheap raw materials for extraction. The effect of large base area and less pollution sources

Inactive Publication Date: 2017-02-22
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the domestic and foreign research on the development of tartary buckwheat resources is limited to the extraction and research of flavonoids, and there are few reports on the study of polysaccharides, an important functional component, and even less research on the comprehensive utilization of tartary buckwheat polysaccharides and flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 The joint extraction method of rutin and polysaccharides in tartary buckwheat seeds comprises the following steps:

[0020] (1) Add distilled water to the freshly harvested tartary buckwheat grains, and obtain extract A and tartary buckwheat dregs through leaching; extract solution A is obtained by suction filtration to obtain filtrate A; add distilled water to tartary buckwheat dregs, and extract Extract B, the extract B is suction filtered to obtain filtrate B; combine filtrate A and filtrate B to obtain suction filtrate.

[0021] Among them: the extraction conditions refer to the solid-liquid mass volume ratio (kg / L) of 1:5, the temperature of 70°C, and the extraction time of 1.5h.

[0022] (2) The filtrate was left to stand at 8°C for 6 hours and then centrifuged at a rate of 4000r / min to obtain precipitate A and supernatant A respectively; the precipitate A was dried at 70°C for 8 hours to obtain tartary buckwheat rutin with a purity of 45.46%. crude pro...

Embodiment 2

[0025] Embodiment 2 The joint extraction method of rutin and polysaccharide in tartary buckwheat seeds comprises the following steps:

[0026] (1) Add distilled water to the freshly harvested tartary buckwheat grains, and obtain extract A and tartary buckwheat dregs through leaching; extract solution A is obtained by suction filtration to obtain filtrate A; add distilled water to tartary buckwheat dregs, and extract Extract B, the extract B is suction filtered to obtain filtrate B; combine filtrate A and filtrate B to obtain suction filtrate.

[0027] Among them: the extraction conditions refer to the solid-liquid mass volume ratio (kg / L) of 1:8, the temperature of 75°C, and the extraction time of 1 hour.

[0028] (2) After standing still at 5°C for 8h, the filtrate was centrifuged at a rate of 3000r / min to obtain precipitate A and supernatant A respectively; the precipitate A was dried at 72°C for 6h to obtain tartary buckwheat rutin with a purity of 50.17%. Crude.

[0029]...

Embodiment 3

[0031] Example 3 The joint extraction method of rutin and polysaccharides in tartary buckwheat seeds comprises the following steps:

[0032] (1) Add distilled water to the freshly harvested tartary buckwheat grains, and obtain extract A and tartary buckwheat dregs through leaching; extract solution A is obtained by suction filtration to obtain filtrate A; add distilled water to tartary buckwheat dregs, and extract Extract B, the extract B is suction filtered to obtain filtrate B; combine filtrate A and filtrate B to obtain suction filtrate.

[0033] Among them: the extraction conditions refer to the solid-liquid mass volume ratio (kg / L) of 1:10, the temperature of 72°C, and the extraction time of 1.2h.

[0034] (2) The filtrate was left to stand at 3°C ​​for 10 hours and then centrifuged at a rate of 5000r / min to obtain precipitate A and supernatant A respectively; the precipitate A was dried at 75°C for 4 hours to obtain tartary buckwheat rutin with a purity of 59.17%. Crude...

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Abstract

The invention relates to a combined extraction method for rutin and polysaccharide in buckwheat seeds. The method comprises the following steps that 1, distilled water is added into the newly-harvested full buckwheat seeds, and after extraction and suction filtration are conducted, suction filtrate is obtained; 2, the suction filtrate is subjected to standing and then centrifuged, a precipitate A and supernatant liquid A are obtained separately, the precipitate A is dried, and then a crude buckwheat rutin product is obtained; 3, ethanol with the volume concentration of 95% is added into the supernatant liquid A until the ethanol concentration of mixed liquid is 60%-70%, the mixed liquid is subjected to standing and then centrifuged, a precipitate B and supernatant liquid B are obtained separately, the precipitate B is dried, and then a crude buckwheat polysaccharide product is obtained; 4, the supernatant liquid B is subjected to reduced pressure concentration to recycle ethanol, and then ethanol with the concentration of 75%-85% is obtained. The combined extraction method is simple and efficient, and the rutin and the polysaccharide in the buckwheat seeds can be extracted simultaneously.

Description

technical field [0001] The invention relates to a method for extracting active ingredients in natural products, in particular to a combined extraction method for rutin and polysaccharides in tartary buckwheat seeds. Background technique [0002] Rutin, also known as rutin and purple quercetin, is the 3-O-rutinoside of quercetin. The molecular formula of rutin is C 27 h 30 o 16 ·3H 2 O, the relative molecular weight is 610.51, its properties are yellow crystalline powder or amorphous powder, with bitter taste, melting point 177~178 ℃, soluble in hot water and ethanol. Rutin is a wide source of flavonoids. As the main component of flavonoids, rutin can not only be used as an antioxidant and a raw material for functional foods, but also has the effect of reducing capillary fragility and improving microcirculation. It is mainly used in the adjuvant treatment of diabetes and hypertension in clinical practice. Polysaccharides, also known as polysaccharides, are a class of com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C08B37/00
CPCC07H1/08C07H17/07C08B37/0003
Inventor 李胜马绍英李宁
Owner GANSU AGRI UNIV
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