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Method for improving expression of neisseria meningitidis fHBP

A Neisseria meningitidis, protein expression technology, applied in the field of genetic engineering, can solve the problem of low expression

Inactive Publication Date: 2017-02-22
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since meningococci only express a small amount of fHBP protein, the expression of fHBP in the form of recombinant protein in Escherichia coli can increase its expression to a certain extent, but the expression of fHBP in the form of recombinant protein is still low and cannot obtain enough protein. The protein meets the needs of subsequent research and development, so the key to improving its expression efficiency to meet the needs of research and production

Method used

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  • Method for improving expression of neisseria meningitidis fHBP
  • Method for improving expression of neisseria meningitidis fHBP
  • Method for improving expression of neisseria meningitidis fHBP

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Embodiment Construction

[0027] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] In the present invention, figure 1 and figure 2 is the comparison of the expression levels of fHBP with different signal peptides under the same induction conditions, image 3 is the relative expression of fHBP with different signal peptides. For details, refer to the following examples.

[0029] The method for expressing recombinant Neisseria meningitidis fHBP protein with different signal peptides provided by the present ...

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Abstract

The invention relates to a method for improving the expression of recombinant neisseria meningitidis fHBP, belonging to the technical field of genetic engineering. According to the method, the neisseria meningitidis fHBP is expressed and recombined by respectively replacing haemophilus influenza P4 outer membrane protein signal peptides, escherichia coli lipoprotein-28 signal peptides and escherichia coli major outer membrane lipoprotein signal peptides, so that compared with the expression amount of the neisseria meningitidis fHBP with original signal peptides, the expression amounts are respectively increased by 2.68+ / -0.32 times, 1.94+ / -0.17 times and 2.91+ / -0.25 times, the problem that the expression amount of the fHBP is low is solved, and a foundation is laid for the process research and development and vaccine application of the recombinant neisseria meningitidis fHBP.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a method for optimizing the signal peptide to improve the expression of recombinant Neisseria meningitidis fHBP protein. Background technique [0002] Neisseria meningitidis (Nm) is a pathogenic Gram-negative bacterium that mainly causes bacterial meningitis and septicemia in children, and is a pathogen that seriously threatens human health. There are five serogroups A, B, C, Y, W135, of which A, C, Y, W135 has developed an effective vaccine with capsular polysaccharide as antigen to prevent the meninges caused by the corresponding serotype Neisseria meningitidis. However, because the capsular polysaccharide of type B meningitidis is homologous to the adhesion molecules of human nerve cells, its immunogenicity is very low and it is not suitable for the preparation of vaccines. The development of polysaccharide antigen vaccine has also become the focus of attention. ...

Claims

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Application Information

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IPC IPC(8): C12N15/70C12N15/66C12N15/31
CPCC12N15/66C12N15/70C07K14/22C12N2810/55
Inventor 寸韡宫悦李智华陈晨李亚东闫玲梅李育中张辉龙琼丁晨肖红剑毕研伟
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI