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Method for increasing content of intracellular ganoderma triterpene in ganoderma lucidum mycelia liquid fermentation

A technology of Ganoderma lucidum mycelium and liquid fermentation, applied in the biological field, can solve problems such as poor safety and economy, and achieve the effects of increasing intracellular Ganoderma lucidum triterpenoid content and easy operation.

Active Publication Date: 2017-02-22
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a method for increasing the content of ganoderma lucidum triterpenoids in the liquid fermentation of ganoderma mycelia. Cultivation, thereby increasing the content of ganoderma triterpenoids in the liquid fermentation of ganoderma liquid mycelium, thus solving the technical problem of poor safety and economic efficiency caused by the existing liquid fermentation of ganoderma lucidum mycelium relying on drugs to stimulate the addition of live heavy metals

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  • Method for increasing content of intracellular ganoderma triterpene in ganoderma lucidum mycelia liquid fermentation
  • Method for increasing content of intracellular ganoderma triterpene in ganoderma lucidum mycelia liquid fermentation

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Embodiment 1

[0039] A method for improving the content of intracellular ganoderma triterpenoids in liquid fermentation of ganoderma mycelia, comprising the following steps:

[0040]Seed cultivation stage: Multi-stage seed cultivation is adopted, so that the ganoderma lucidum mycelium can fully grow and form a liquid fermentation system of ganoderma lucidum mycelium with good growth and strong adaptability, which is suitable for the second stage of variable temperature treatment. The specific operation steps are as follows:

[0041] (1) Activate the mycelia of Ganoderma lucidum with PDA liquid medium, culture at 28°C to 30°C, and shaker speed 120-150r / min for 7 days to obtain the first-grade seed liquid;

[0042] Wherein, each liter of the PDA liquid medium contains 20 g of glucose and 200 g of potato juice, and is sterilized at 115° C. for 25 to 40 minutes.

[0043] (2) Inoculate the primary seed solution obtained in step (1) in the first ganoderma lucidum seed medium according to the inoc...

Embodiment 2

[0052] A method for improving the content of intracellular ganoderma triterpenoids in liquid fermentation of ganoderma mycelia, comprising the following steps:

[0053] Seed culture stage: as embodiment 1

[0054] In the expanded cultivation stage, variable temperature treatment is adopted to induce the ganoderma lucidum mycelium to increase the content of ganoderma lucidum triterpenes by changing the temperature of the fermentation environment and simulating the impact of high and low temperature changes in the field environment on the accumulation of ganoderma lucidum triterpenes in the fermentation cells of ganoderma lucidum mycelium. The specific operation process as follows:

[0055] Fermented at 4°C for 2 hours, fermented at normal temperature (28°C to 30°C) for 22 hours, and then fermented at 4°C for 2 hours, 144 hours of circular culture.

Embodiment 3

[0057] A method for improving the content of intracellular ganoderma triterpenoids in liquid fermentation of ganoderma mycelia, comprising the following steps:

[0058] Seed culture stage: as embodiment 1

[0059] In the expanded cultivation stage, variable temperature treatment is adopted to induce the ganoderma lucidum mycelium to increase the content of ganoderma lucidum triterpenes by changing the temperature of the fermentation environment and simulating the impact of high and low temperature changes in the field environment on the accumulation of ganoderma lucidum triterpenes in the fermentation cells of ganoderma lucidum mycelium. The specific operation process as follows:

[0060] Fermented at 8°C for 2 hours, fermented at normal temperature 28°C to 30°C for 22 hours, and then fermented at 8°C for 2 hours, so cycled for 144 hours.

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Abstract

The invention discloses a method for increasing the content of intracellular ganoderma triterpene in ganoderma lucidum mycelia liquid fermentation. The method comprises the following steps: amplifying a culture period, culturing a ganoderma lucidum mycelia liquid fermentation system for 5-7 days at 28-30 DEG C, and performing high-temperature or low-temperature treatment on the ganoderma lucidum mycelia liquid fermentation system for 1-3 hours every day. As the wild growth environment of ganoderma is simulated, and high-temperature or low-temperature fermentation culture is implemented, high-level accumulation of intracellular ganoderma triterpene of ganoderma can be achieved, on premise that no foreign substance is added, the method is cheap, safe and easy to operate, the content of the intracellular ganoderma triterpene can be remarkably increased, and thus a basis can be made for industrial production of ganoderma triterpene.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for increasing the content of ganoderma lucidum triterpenoids in the bag of ganoderma lucidum mycelium liquid fermentation. Background technique [0002] Ganoderma lucidum triterpenoids are a class of triterpenoids found in Ganoderma lucidum, that is to say, Ganoderma lucidum triterpenes are the main chemical and medicinal components of Ganoderma lucidum. Ganoderma lucidum triterpenes contained in Ganoderma lucidum have particularly significant physiological activities. In a highly competitive society, excessive work, changes in lifestyle or environmental pollution can cause serious damage to human health, and Ganoderma lucidum triterpenes have great application value. [0003] Studies on the physiological and biochemical processes of Ganoderma lucidum have shown that triterpenoids (ganoderic acid) belong to the secondary metabolites of Ganoderma lucidum. I...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12N1/14C12R1/645
CPCC12N1/14C12P33/00
Inventor 胡学博李嘉伟胡延如
Owner HUAZHONG AGRICULTURAL UNIVERSITY