Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of no-mark insulin sensor based on BiOBr/Ag2S composite material

A composite material and insulin technology, which is applied in analytical materials, material inspection products, biological material analysis, etc., can solve problems such as unit insulin function decline, hinder insulin secretion ability, etc., achieve ultra-sensitive detection, reduce detection limit, and expand linear range Effect

Active Publication Date: 2017-02-22
UNIV OF JINAN
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in some diseases, severe burns, etc., the human body may experience a decline in the function of unit insulin and the impairment of insulin secretion by pancreatic β cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 a kind of based on BiOBr / Ag 2 Preparation method of S composite material label-free insulin sensor

[0022] (1) Divide 2.5×0.8 cm 2 The ITO electrode was submerged in acetone, water, ethanol and water at 50°C and ultrasonically cleaned for 30 min respectively, and then dried with high-purity nitrogen; 5 μL of 1 mg / mL BiOBr material suspension was drop-coated on the surface of the ITO electrode , after natural drying, the electrode modified by BiOBr material was placed in a muffle furnace, calcined at 300 ° C for 30 min, and then cooled to room temperature;

[0023] (2) Take 3 μL of 0.05 mol / L thioglycolic acid solution and drop-coat it on the ITO electrode modified by BiOBr material, rinse with ultrapure water after reacting for 20 minutes, and let it dry naturally;

[0024] (3) Take 3 μL of 0.01 mol / L AgNO 3 The aqueous solution was drip-coated on the surface of the electrode, and rinsed with ultrapure water after 30 min in the dark; then, 3 μL of 0.1 m...

Embodiment 2

[0028] Embodiment 2 A kind of based on BiOBr / Ag 2 Preparation method of S composite material label-free insulin sensor

[0029] (1) Divide 2.5×0.8 cm 2 The ITO electrode was submerged in acetone, water, ethanol and water at 50°C for 30 min, and then blown dry with high-purity nitrogen; 10 μL of 4 mg / mL BiOBr material suspension was drop-coated on the surface of the ITO electrode , after natural drying, the electrode modified by BiOBr material was placed in a muffle furnace, calcined at 400 ° C for 60 min, and then cooled to room temperature;

[0030] (2) Take 3 μL of 0.1 mol / L thioglycolic acid solution and drop-coat it on the BiOBr-modified ITO electrode. After reacting for 30 minutes, wash it with ultrapure water and let it dry naturally;

[0031] (3) Take 3 μL of 0.05 mol / L AgNO 3 The aqueous solution was drip-coated on the surface of the electrode, and rinsed with ultrapure water after 30 min in the dark; then, 3 μL of 0.1 mol / L Na 2 The S solution was reacted on the s...

Embodiment 3

[0035] Embodiment 3 a kind of based on BiOBr / Ag 2 Preparation method of S composite material label-free insulin sensor

[0036] (1) Divide 2.5×0.8 cm 2 The ITO electrode was submerged in acetone, water, ethanol and water at 50°C for 30 min, and then blown dry with high-purity nitrogen; 15 μL of 6 mg / mL BiOBr material suspension was drop-coated on the surface of the ITO electrode , after natural drying, the electrode modified by BiOBr material was placed in a muffle furnace, calcined at 500 ° C for 90 min, and then cooled to room temperature;

[0037] (2) Take 3 μL of 0.2 mol / L thioglycolic acid solution and drop-coat it on the BiOBr material-modified ITO electrode. After reacting for 40 minutes, wash it with ultrapure water and let it dry naturally;

[0038] (3) Take 3 μL of 0.1 mol / L AgNO 3The aqueous solution was drip-coated on the surface of the electrode, and rinsed with ultrapure water after 30 min in the dark; then, 3 μL of 0.1 mol / L Na 2 The S solution was reacted o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a no-mark insulin sensor based on a BiOBr / Ag2S composite material, and belongs to the technical field of novel functional nano composite materials and the detection of biosensors. Based on the characteristics that a micron flower-shaped BiOBr material is large in specific surface area and stable in performance, after the BiOBr material is modified by using thioglycollic acid, through alternately adsorbing AgNO3 and Na2S, an Ag2S nano material is enabled to grow in situ on the surface of the BiOBr material; the BiOBr / Ag2S composite material which is excellent in photoelectrochemical properties is obtained; through a layer-by-layer self-assembly method, an insulin antibody, bovine serum albumin and insulin are assembled on the BiOBr / Ag2S composite material; the ultra-sensitive detection on the insulin is realized by utilizing the excellent photoelectrical activity of the BiOBr / Ag2S and a specific binding mechanism between the insulin antibody and the insulin; the no-mark insulin sensor has an important meaning to the analysis and the detection of the insulin.

Description

technical field [0001] The present invention relates to a kind of based on BiOBr / Ag 2 Fabrication method of S-composite material label-free insulin sensor, using micro-flower-like BiOBr as carrier, thioglycolic acid as linker, and in situ growth of Ag 2 S nanoparticles to obtain BiOBr / Ag 2 The invention discloses an S composite material and prepares a photoelectrochemical sensor for detecting insulin, which belongs to the technical field of new functional nano composite material and biosensor detection. Background technique [0002] Insulin is an important hormone in the human body that promotes the efficient use of glucose by the body. However, in some diseases, severe burns, etc., the human body may experience a decrease in the function of unit insulin and an adverse situation in which the islet β cells hinder the ability to secrete insulin. Therefore, in view of these two situations, timely and accurate detection of the level of insulin in serum is of great significanc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N33/74
CPCG01N27/3275G01N33/74G01N2333/62
Inventor 范大伟王欢任祥王耀光魏琴杜斌吴丹马洪敏王浩源
Owner UNIV OF JINAN