A method for rapid determination of sulfonamide antibiotic residues in animal muscle tissue based on magnetic separation
A technology for rapid determination of animal muscle, applied in the field of sulfonamide antibiotic residues, can solve the problems of complex sample preparation methods, and achieve the effects of low cost, high sensitivity, and simple pretreatment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] Example 1: 1. Preparation of fish body samples: carp tissues were homogenized and stored in a refrigerator at -20°C;
[0019] 2. Extraction: Melt the surimi at room temperature, weigh 2g sample into a 50mL centrifuge tube with a lid, add 5mL mixed solution to fully infiltrate, vortex and mix; add Fe 3 O 4 Magnetic material mixture (consisting of 2g anhydrous magnesium sulfate, 0.5g sodium chloride and 100mg Fe 3 O 4 Magnetic particle composition), vortex and mix immediately to prevent anhydrous magnesium sulfate from agglomerating, and quickly separate under the action of an external magnet;
[0020] 3. Purification: Pipette 1mL of the sample extract supernatant into a 5mL centrifuge tube, concentrate by nitrogen blowing, add 1mL 1‰ formic acid solution to dissolve the residue, transfer the solution to a 5mL centrifuge tube containing 1mL n-hexane, vortex and shake After centrifugation, the upper n-hexane phase was discarded, the lower clear liquid was sucked and filtered thr...
Embodiment 2
[0022] Example 2: Add standard samples of sulfonamide antibiotics at three levels of 0.1, 0.25, and 0.5 mg / kg to blank fish samples, extract and purify the samples, calculate the recovery rate, and repeat 5 times.
[0023] The recovery rate of standard addition for carp muscle is as follows:
[0024]
[0025]
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com