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A methylglyoxal and detection method technology, applied in the field of measurement, can solve the problems of uneven quality of Manuka honey, achieve the effects of shortened detection time, simple operation steps, high recovery rate and precision
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Problems solved by technology
[0003] Due to the excellent antibacterial properties of Manuka honey, in recent years, Manuka honey has become more and more popular with consumers, and its import volume has increased significantly. At the same time, its price is higher than other honeys, but the price of Manuka honey on the market quality varies
According to media reports, Australia and New Zealand produce about 1,700 t to 2,000 t of Manuka honey each year, but globally, more than 10,000 tons of honey are sold in the name of Manuka each year, and about 80% of Manuka honey exists Quality issues
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Embodiment 1
[0022] The methylglyoxal detection method in the honey that the present invention relates to is divided into: extraction, derivation and analysis three steps:
[0023] (1) Extraction: Weigh 1 g (accurate to 0.001 g) of a brand of Manuka honey from New Zealand into a 100 mL volumetric flask, add about 80 mL of water to dissolve, dilute to the mark with water, and shake well.
[0024] Draw 10.0 mL of the above solution into a 100 mL volumetric flask, add water to dilute to the mark, shake well, and set aside.
[0025] (2) Derivatization: Pipette 1.0 mL of sample solution into a 15 mL centrifuge tube, add 1.0 mL of 2,3-diaminonaphthalene solution, and shake well.
[0026] Draw 1.0 mL each of the standard series of working solutions 0.05, 0.10, 0.20, 0.50, 0.80, and 1.00 μg / mL, add 1.0 mL of 2,3-diaminonaphthalene solution (1 mg / mL), derivatize with the sample at the same time, and do a blank experiment at the same time.
[0027] (3) Analysis: inject the sample solution and stand...
Embodiment 2
[0035] The method for detecting the content of methylglyoxal in honey involved in this example is the same as Example 1, except that a certain brand of Australian honey mixed with various flowers is used as a sample to prepare a sample solution, and the results are as follows:
[0036]
Embodiment 3
[0038] The present embodiment carries out recovery rate measurement with the honey that does not contain methylglyoxal through measuring, has measured the recovery rate of methylglyoxal of different addition levels, precision specific result is as follows:
[0039] Table 3 Recovery, precision results ( n =6)
[0040]
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Abstract
The invention discloses a detection method of methylglyoxal in honey. The detection method comprises three steps of extraction, derivation and analysis. Compared with the prior art, the detection method uses a safe and stable derivatization reagent and adopts a 2,3-diaminonaphthalene derivatization technique; moreover, the 2,3-diaminonaphthalene derivatization technique is improved and an extracted sample solution is put into a liquid chromatograph tandem mass spectrometer to carry out qualitative and quantitative analysis and measurement; pretreatment operating steps are simple and convenient; a reaction is mild; a low-concentration honey solution can reduce the damage of a sugar substrate to a chromatographic column; the method of the methylglyoxal is complete; the detection limit is low; the recovery rate and the precision are high, and the accurate detection and positive corroboration can be carried out on imported manuka honey.
Description
Technical field: [0001] The invention belongs to the technical field of measurement, and in particular relates to a method for detecting the content of methylglyoxal in honey, which has the advantages of simple and convenient pretreatment operation steps, high recovery rate and precision, and can quickly, accurately and effectively measure. Background technique: [0002] Manuka is a natural tea tree. Manuka leaves and bark are natural herbal medicines used by the Maori, the aborigines of New Zealand, for hundreds of years. Since the 1990s, scientists began to study the special effects of Manuka honey, and gradually discovered that Manuka honey has anti-bacterial, fungal, anti-viral, and anti-oxidative effects, and can promote wound healing. This non-hydrogen peroxide antimicrobial activity is thought to be mainly derived from methylglyoxal (MGO) in honey, which is also considered a key indicator of the quality of Manuka honey and according to MGO. Manuka honey was graded fo...
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