Detection method of methylglyoxal in honey
A methylglyoxal and detection method technology, applied in the field of measurement, can solve the problems of uneven quality of Manuka honey, achieve the effects of shortened detection time, simple operation steps, high recovery rate and precision
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Embodiment 1
[0022] The methylglyoxal detection method in the honey that the present invention relates to is divided into: extraction, derivation and analysis three steps:
[0023] (1) Extraction: Weigh 1 g (accurate to 0.001 g) of a brand of Manuka honey from New Zealand into a 100 mL volumetric flask, add about 80 mL of water to dissolve, dilute to the mark with water, and shake well.
[0024] Draw 10.0 mL of the above solution into a 100 mL volumetric flask, add water to dilute to the mark, shake well, and set aside.
[0025] (2) Derivatization: Pipette 1.0 mL of sample solution into a 15 mL centrifuge tube, add 1.0 mL of 2,3-diaminonaphthalene solution, and shake well.
[0026] Draw 1.0 mL each of the standard series of working solutions 0.05, 0.10, 0.20, 0.50, 0.80, and 1.00 μg / mL, add 1.0 mL of 2,3-diaminonaphthalene solution (1 mg / mL), derivatize with the sample at the same time, and do a blank experiment at the same time.
[0027] (3) Analysis: inject the sample solution and stand...
Embodiment 2
[0035] The method for detecting the content of methylglyoxal in honey involved in this example is the same as Example 1, except that a certain brand of Australian honey mixed with various flowers is used as a sample to prepare a sample solution, and the results are as follows:
[0036]
Embodiment 3
[0038] The present embodiment carries out recovery rate measurement with the honey that does not contain methylglyoxal through measuring, has measured the recovery rate of methylglyoxal of different addition levels, precision specific result is as follows:
[0039] Table 3 Recovery, precision results ( n =6)
[0040]
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