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Compositions and methods for increasing plant growth and yield

A plant growth, plant technology, applied in the field of increasing plant growth and yield and composition, can solve the problems of technical labor and the like

Inactive Publication Date: 2017-02-22
DONALD DANFORTH PLANT SCI CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such selective breeding techniques have several drawbacks, namely, that these techniques are generally laborious and produce plants that often contain heterologous genetic components, which may not always result in the desired trait being passed down from the parental plants

Method used

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  • Compositions and methods for increasing plant growth and yield
  • Compositions and methods for increasing plant growth and yield
  • Compositions and methods for increasing plant growth and yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1 - Discovery of 118 transcription factors proposed to regulate C4 photosynthesis

[0102] Rice and maize leaves were partitioned along developmental gradients, and RNA was extracted from each leaf segment. This RNA was sequenced using RNA-Seq to derive developing leaf gradients to generate transcriptome data. This transcriptome data is organized into 30 different clusters based on the expression profile of each gene. The expression profiles of 2517 maize transcription factors annotated in the Grassius database (www.grassius.org) were then analyzed.

[0103] The following criteria were applied to discover transcription factors that might be involved in the regulation of C4 photosynthesis.

[0104] 1. TF is expressed above background noise in leaves

[0105] 2. There is a one-to-one corresponding orthologous gene in rice (based on synteny and sequence similarity)

[0106] 3. There are persistent differential gene expression profiles in bundle sheath vs. mesop...

Embodiment 2

[0116] Example 2 - Filtering 118 transcription factors to determine test prioritization

[0117] To prioritize the TFs described in Example 1 for further testing, additional filtering was performed. The expression levels of each maize TF and corresponding rice TF in Table 1 were compared individually on 15 gradients in the Unified Developmental Model (UDM). The ratio of maize expression to rice expression levels was calculated for each TF pair at each gradient. Calculate the maximum and minimum ratios for each TF pair. Then 10 of the 118 TFs in Table 1 with the largest maize:rice expression ratio were selected, and 10 of the 118 TFs in Table 1 with the smallest maize:rice expression ratio were also selected.

[0118] The filtering process described herein resulted in the selection of 20 maize TFs and their corresponding rice orthologs that displayed widely divergent expression profiles between rice and maize. These TFs are listed in Table 2. Not being shown by theory, it i...

Embodiment 3

[0123] Example 3 - Derivation of cis-regulatory elements may drive expression in a cell-specific manner

[0124] A key feature of C4 photosynthesis is the partitioning of photosynthetic activity between 2 neighboring cell types, and in maize this occurs primarily through transcriptional control. In dicot systems, it has been shown that cis-elements from C4 plants can be recognized and confer the same cell-specific expression pattern in C3 plants. Thus, one mechanism for differential gene expression appears to be the exploration of existing cis-elements that are conserved between C3 and C4 species. To identify novel cis-acting elements that drive cell-type-specific gene expression in C4 photosynthetic differentiation, promoter sequences from the photosynthetic cluster, which includes most C4 carbon shuttling genes, were compared between maize and rice. Sequences within 3 kb upstream of the initiation codons of all maize and rice genes in these clusters were examined and then a...

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Abstract

Compositions and methods for increasing plant yield are disclosed. Compositions comprise transcription factors that find use in modulating the expression of a gene or nucleotide sequence of interest in a plant. Additionally, promoters and cis-regulatory elements are disclosed that may be used to drive the expression of a nucleotide sequence of interest in a plant. Methods for the use of such compositions as well as transformed plants are disclosed.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application 61 / 991,949, filed May 12, 2014, and US Provisional Application 62 / 023,432, filed July 11, 2014, which are incorporated herein by reference in their entirety. [0003] About the sequence listing submitted as a text file via EFS-WEB [0004] The official text of the sequence listing is submitted electronically in ASCII format through EFS-Web, the file name is 462655SEQLIST.txt, which was generated on May 7, 2015, with a size of 1,274KB, and submitted together with the instructions. The sequence listing contained in this document in ASCII format is a part of this specification and is incorporated by reference in its entirety. [0005] field of invention [0006] The present invention relates to methods and compositions for controlling the expression of genes involved in plant growth and development. [0007] Background of the invention [0008] The ever-inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N15/113A01H5/00
CPCC07K14/415C12N15/8216C12N15/8261C12N15/8269Y02A40/146C12N15/8222C12N2830/002
Inventor L·王T·P·布鲁特内尔T·C·莫克勒D·W·布莱恩特
Owner DONALD DANFORTH PLANT SCI CENT
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