Cell strain with sterol carrier protein (SCP) stable transformation of prodenia litura and preparation method and application of cell strain
A sterol and transporter technology of Spodoptera litura, which is applied to biochemical equipment and methods, genetically modified cells, and cells modified by introducing foreign genetic material, etc. Cells, the effect of blocking the growth of insects
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[0058] Before setting forth the method of the present invention, the following abbreviations are explained and described:
[0059] Ham's F-12 medium:
[0060] NCBI: National Center for Biotechnology Information
[0061] SlSCPx: Spodoptera litura SCPx
[0062] pro-SlSCPx-2: Spodoptera litura SCPx-2 precursor
[0063] ORF: open reading frame
[0064] DH5α: bacterial strain name (Escherichia coli competent cells)
[0065] Opti-MEM: Medium
[0066] Hygromycin B: hygromycin
[0068] DMSO: Dimethylsulfoxide
[0069] SCP-2 Gene Number: FJ986464
[0070] Literature source:
[0071] Guo, X.R., Zheng, S.C., Liu, L. and Feng, Q.L. The sterol carrier protein 2 / 3-oxoacyl-CoA thiolase (SCPx) is involved in cholesterol uptake in the midgutof Spodoptera litura: gene cloning, expression, localization and functional analyzes .BMC Mol.Biol.10,102-120(2009)
[0072] Guo Xingrong, Zheng Sichun, Liu Lin, Feng Qili Sterol transporter 2 / 3 acyl-CoA thiolase (S...
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