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Blood analysis device and blood analysis method

A blood analysis and blood technology, applied in the direction of suspension and porous material analysis, analysis materials, measuring devices, etc., can solve the problem of difficult to make correct judgments on blood samples

Active Publication Date: 2020-12-15
SYSMEX CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The detection method disclosed in Patent Document 1 uses the number of polycyclic bodies, so it may be difficult to make a correct judgment on this blood sample

Method used

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  • Blood analysis device and blood analysis method
  • Blood analysis device and blood analysis method
  • Blood analysis device and blood analysis method

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Embodiment Construction

[0041] Preferred embodiments of the present invention will be described below with reference to the accompanying drawings.

[0042] In this embodiment, it is explained that a group including malaria-infected erythrocytes is identified based on the fluorescence intensity and forward scattered light intensity generated when a measurement sample prepared from a blood sample and a reagent is irradiated, and whether the Plasmodium infected with the erythrocytes is Plasmodium falciparum is determined. blood analysis device.

[0043] (Structure of blood analysis device)

[0044] Refer below figure 1The configuration of the blood analysis device will be described. The blood analysis device 1 has a measurement unit 2 and an analysis unit 3 . The measurement unit 2 takes in a blood sample, prepares a measurement sample from the blood sample, and performs optical measurement on the measurement sample. The analysis unit 3 processes the measurement data obtained by the measurement unit...

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Abstract

A blood analysis device comprising: a sample preparation unit for preparing a measurement sample using a diluent for shrinking red blood cells, a staining dye for staining nucleic acid, and a blood sample; a detection unit for detecting Fluorescence intensity and scattered light intensity obtained from the prepared assay sample; output means; analysis means, which determines, based on the fluorescence intensity and said scattered light intensity, a population comprising erythrocytes infected with P. The scattered light intensity distribution of the particles belonging to the erythrocyte group of Plasmodium annulare is obtained, and the output unit outputs information on infection by Plasmodium falciparum.

Description

technical field [0001] The present invention relates to a blood analysis device and a blood analysis method for analyzing a blood sample infected with Plasmodium using a measurement sample prepared from blood. Background technique [0002] Japanese Patent Laid-Open (Japanese Patent Laid-Open) No. 2006-304774 discloses a method for detecting red blood cells infected with Plasmodium (hereinafter referred to as malaria-infected red blood cells). This detection method mixes a reagent (which contains a dye that stains DNA more strongly than RNA and partially dissolves the red blood cell membrane to allow the dye to permeate in a state that contains Plasmodium inside) with a blood sample, Prepare the measurement sample, introduce the measurement sample into the flow cell of the flow cytometer, irradiate the measurement sample flowing through the flow cell with light, detect the scattered light and fluorescence emitted by the measurement sample, and take the scattered light intensi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/49
CPCG01N21/49G01N21/6486G01N33/4915G01N2015/1486G01N2015/1488G01N2015/1493G01N2333/445G01N2015/1402G01N2015/1477G01N15/147Y02A50/30G01N2015/1019G01N15/01G01N15/075G01N2015/012G01N33/5091G01N33/5094G01N33/582G01N2021/4707G01N2021/6439G01N1/30G01N15/06G01N21/47G01N21/6428G01N2001/302G01N2201/0612G01N2201/12
Inventor 铃木裕义井塚宗久木村考伸
Owner SYSMEX CORP