Application of 1-pyrenecarboxylic acid as pH fluorescence probe

A fluorescent probe, pyrene formic acid technology, applied in the field of optical analysis and detection, can solve the problems of low probe sensitivity, wide response range, discomfort, etc., and achieve the effects of strong selectivity, wide application range and good anti-interference ability.
CN106483113AInactive Publication Date: 2017-03-08SOUTH CHINA NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA NORMAL UNIVERSITY
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of photoanalysis and detection and particularly relates to application of 1-pyrenecarboxylic acid as a pH fluorescence probe to detection of a pH value of the solution and a pH value determination method based on the 1-pyrenecarboxylic acid fluorescence probe. The 1-pyrenecarboxylic acid is good in water solubility and capable of detecting the pH value in a water solution, thereby being a sensitive pH fluorescence probe; the 1-pyrenecarboxylic acid makes positive response to a to-be-determined solution with the pH value ranging from 2.0-6.0, thereby being applicable to pH value determination in an acid system; common metal ions have little effect on the characteristic of the 1-pyrenecarboxylic acid fluorescence probe for recognition of hydrogen ions. The application of the 1-pyrenecarboxylic acid as the pH fluorescence probe has the advantages that the shortcomings of low sensitivity, poor selectivity and proneness to being interfered by metal ions in an existing pH measurement method are overcome, and the 1-pyrenecarboxylic acid fluorescence probe is high in accuracy and sensitivity and wide in application range when used for detecting pH values of the solutions; currently, the 1-pyrenecarboxylic acid is commercialized, so that all-round popularization is facilitated.
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Description

Technical field

[0001] The invention relates to the application of 1-pyrenecarboxylic acid as a fluorescent probe in detecting the pH value of a solution, and belongs to the technical field of optical analysis and detection. Background technique

[0002] The progress or completion of chemical reactions and many important physiological processes of cells and organelles are closely related to pH. Therefore, the accurate measurement of pH is very important for chemical and biological research. The existing pH measurement methods mainly include electrode method, indicator method and fluorescence spectroscopy.

[0003] The existing pH measurement methods also have the following shortcomings: (1) The electrode method is not suitable for in-vivo pH detection due to electrochemical interference and possible mechanical damage; (2) The indicator method has a wide color range, resulting in detection sensitivity. (3) Fluorescence spectroscopy is a detection method based on optical signals. C...

Claims

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