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Application of 1-pyrenecarboxylic acid as pH fluorescence probe

A fluorescent probe, pyrene formic acid technology, applied in the field of optical analysis and detection, can solve the problems of low probe sensitivity, wide response range, discomfort, etc., and achieve the effects of strong selectivity, wide application range and good anti-interference ability.

Inactive Publication Date: 2017-03-08
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] The existing pH measurement methods also have the following deficiencies: (1) The electrode method is not suitable for pH detection in vivo due to defects such as electrochemical interference and possible mechanical damage; (3) Fluorescence spectroscopy is a detection method based on optical signals. Compared with the previous two methods, it has the advantages of high sensitivity, good selectivity, real-time and in-situ detection, etc. Higher attention, but the common problem of pH fluorescent probes is that they have a wide response range to hydrogen ions, which leads to low sensitivity, poor selectivity, and easy interference by metal ions.

Method used

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  • Application of 1-pyrenecarboxylic acid as pH fluorescence probe
  • Application of 1-pyrenecarboxylic acid as pH fluorescence probe
  • Application of 1-pyrenecarboxylic acid as pH fluorescence probe

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Experimental program
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Effect test

Embodiment 1

[0030] Fluorescence recognition of 1-pyrene acid for different pH values:

[0031] Prepare different pH values ​​containing 1-pyrene formic acid (pH = 11.07, 9.92, 8.97, 8.05, 7.00, 6.40, 5.81, 5.22, 5.00, 4.61, 4.43, 4.04, 3.69, 3.43, 2.90, 2.73, 2.32, 2.20) Buffer solution (buffer solution concentration is 2.0×10 -3 mol / L, where the concentration of 1-pyrenecarboxylic acid is 2.0×10 -5 mol / L. Using 350nm as the excitation wavelength, measure and plot the fluorescence spectra of 1-pyrenecarboxylic acid in buffer solutions with different pH values ​​(such as figure 1 Shown).

[0032] Such as figure 1 As shown, when excited with 350nm wavelength, the emission wavelength is the highest near 382nm and 401nm, and when the fluorescence emission wavelength of 1-pyreneformic acid is at 382nm, as the pH value decreases, 1-pyreneformic acid reacts to hydrogen ion The response of 1-pyreneformic acid shows fluorescence quenching; at 401nm, as the pH value decreases, the response of 1-pyrenec...

Embodiment 2

[0036] Selectivity of 1-pyrenecarboxylic acid fluorescent probe to hydrogen ion:

[0037] To a buffer solution with a pH of 5.0 (wherein the concentration of 1-pyrene formic acid is 2.0×10 -5 mol / L), dropwise containing Al 3+ Metal ion solution to make Al in the buffer solution 3+ The concentration of 1.0×10 -4 mol / L. Use the same method to configure each containing Ba 2 + , Cd 2+ , Cr 3+ , Co 2+ , Cu 2+ , Fe 3+ , Hg 2+ , Ni 2+ , Pb 2+ Buffer solution (the corresponding metal ion concentration in each buffer solution is 1.0×10 -4 mol / L, the concentration of 1-pyrenecarboxylic acid is 2.0×10 -5 mol / L).

[0038] Using 350nm as the excitation wavelength, measure the fluorescence spectrum of each of the above-mentioned test solutions, and calculate I 401 / I 382 , In terms of fluorescence intensity ratio I 401 / I 382 Corresponding to different metal ions mapping (such as Figure 4 Shown).

[0039] Such as Figure 4 As shown, the fluorescence intensity ratio of 1-pyrenecarboxylic acid in e...

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Abstract

The invention belongs to the technical field of photoanalysis and detection and particularly relates to application of 1-pyrenecarboxylic acid as a pH fluorescence probe to detection of a pH value of the solution and a pH value determination method based on the 1-pyrenecarboxylic acid fluorescence probe. The 1-pyrenecarboxylic acid is good in water solubility and capable of detecting the pH value in a water solution, thereby being a sensitive pH fluorescence probe; the 1-pyrenecarboxylic acid makes positive response to a to-be-determined solution with the pH value ranging from 2.0-6.0, thereby being applicable to pH value determination in an acid system; common metal ions have little effect on the characteristic of the 1-pyrenecarboxylic acid fluorescence probe for recognition of hydrogen ions. The application of the 1-pyrenecarboxylic acid as the pH fluorescence probe has the advantages that the shortcomings of low sensitivity, poor selectivity and proneness to being interfered by metal ions in an existing pH measurement method are overcome, and the 1-pyrenecarboxylic acid fluorescence probe is high in accuracy and sensitivity and wide in application range when used for detecting pH values of the solutions; currently, the 1-pyrenecarboxylic acid is commercialized, so that all-round popularization is facilitated.

Description

Technical field [0001] The invention relates to the application of 1-pyrenecarboxylic acid as a fluorescent probe in detecting the pH value of a solution, and belongs to the technical field of optical analysis and detection. Background technique [0002] The progress or completion of chemical reactions and many important physiological processes of cells and organelles are closely related to pH. Therefore, the accurate measurement of pH is very important for chemical and biological research. The existing pH measurement methods mainly include electrode method, indicator method and fluorescence spectroscopy. [0003] The existing pH measurement methods also have the following shortcomings: (1) The electrode method is not suitable for in-vivo pH detection due to electrochemical interference and possible mechanical damage; (2) The indicator method has a wide color range, resulting in detection sensitivity. (3) Fluorescence spectroscopy is a detection method based on optical signals. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 马立军吕足唐健陈嘉韵李宛珊谢政
Owner SOUTH CHINA NORMAL UNIVERSITY
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