Gluconacetobacter with high brown pigment yield and method for preparing brown pigment by aid of gluconacetobacter

A technology of gluconacetobacter and brown pigment, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc.

Inactive Publication Date: 2017-03-15
陈福生
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Gluconacetobacter with high brown pigment yield and method for preparing brown pigment by aid of gluconacetobacter
  • Gluconacetobacter with high brown pigment yield and method for preparing brown pigment by aid of gluconacetobacter
  • Gluconacetobacter with high brown pigment yield and method for preparing brown pigment by aid of gluconacetobacter

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Embodiment 1

[0022] Example 1 Influence of fermentation time on the content of brown pigment in fermentation broth: According to the method described in the manual, the content of brown pigment in the fermentation broth of Acetobacter botrytis FBFS 97-2 under different fermentation times was detected by spectrophotometer (440 nm), and it was found that the fermentation The color depth of the liquid first increased and then decreased with the fermentation time. The color on the 17th day was the darkest, and the absorbance at 440 nm was 20.08 ( Figure 2-Figure 3 ).

[0023] The method for detecting the brown pigment content in the fermentation broth in the above example includes the following steps: the fermentation broth is firstly centrifuged at a high speed (10000 r / min, 10 min) to remove the bacterial cells, and then properly diluted and then detected with a spectrophotometer at 440 nm.

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Abstract

The invention discloses an acetic acid bacterium for producing brown pigment and application of the acetic acid bacterium to preparing the brown pigment, and provides gluconacetobacter. A gluconacetobacter strain FBFS 97-2 is preserved in the China Center for Type Culture Collection on 14th September, 2016, and a preservation number of the gluconacetobacter strain is CCTCC M2016481. The gluconacetobacter has the advantages that the gluconacetobacter FBFS 97-2 can be subjected to liquid-state fermentation under the conditions of the temperature of 30 DEG C and the speed of 160 r / min for 17 d, and the light absorbance of the brown pigment in culture solution can reach 20.08 under the condition of the maximum absorption wavelength of 440 nm; the shortcoming of deficiency of report on related research can be overcome.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and relates to a high brown pigment-producing Gluconacetobacter ( Gluconacetobacter sp.) FBFS 97-2 and its preparation method of brown pigment. Background technique [0002] Pigment, also known as colorant, refers to the substance that can color the dyed object and can be used to improve the color of the article. It is widely used in food, pharmaceutical, cosmetic, textile and other industrial fields. [0003] Pigments are usually divided into synthetic pigments and natural pigments according to their production methods. Synthetic pigments are widely used because of their bright color, strong tinting strength, stable properties and low price. However, with the development of society and the improvement of living standards, people's requirements for the safety of food, medicine and daily necessities are getting higher and higher, and many synthetic pigments are also banned or limited in use due ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C09B61/00C12R1/01
CPCC09B61/00C12P1/04C12N1/205C12R2001/01
Inventor 陈福生陈亨业
Owner 陈福生
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