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A kind of in vitro cultivation method of Star of Bethlehem in South Africa

A technology of in vitro culture and culture medium, which is applied in the field of in vitro culture of the Star of Bethlehem in South Africa, can solve the problems of low reproduction coefficient and long reproduction cycle, achieve high transplant survival rate, low production cost, and solve extremely perishable diseases. Effect

Active Publication Date: 2018-08-03
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is exactly in order to solve above-mentioned technical problem, and provides a kind of in vitro cultivation method of Star of Bethlehem in South Africa, solves its low reproduction coefficient, long breeding cycle, can't obtain enough high-quality Star of Bethlehem seedlings in a short time To solve the problem, provide enough high-quality seedlings for its growers, increase their production efficiency, and lay a solid foundation for the large-scale promotion and planting of Star of Bethlehem in Yunnan Province

Method used

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  • A kind of in vitro cultivation method of Star of Bethlehem in South Africa
  • A kind of in vitro cultivation method of Star of Bethlehem in South Africa

Examples

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Embodiment 1

[0033] A method for in vitro culturing of the Star of Bethlehem in South Africa, comprising the following steps:

[0034] 1) Pretreatment of scales: On a sunny day, go to the Star of Bethlehem Seedling Base in South Africa in Yunnan Province to pick up the bulbs, select healthy and plump bulbs, cut off the rotten and dry scales on the surface, and select clean and complete scales inside. Soak in water with 1 drop of detergent added for 3 minutes, rinse with clean water for 10 minutes, then rinse twice with pure water, then transfer to an ultra-clean workbench and treat with 75% ethanol for 30 seconds, rinse with sterile water Remove ethanol, and then sterilize with 0.1% mercuric chloride aqueous solution for 20 minutes. During the sterilization process, use tweezers to repeatedly stir the scales to make them completely sterilized, and then rinse them with sterile water for 5 times;

[0035] 2) Induction culture: cut the scales treated in step 1) into small squares of 2 cm × 3 ...

Embodiment 2

[0042] A method for in vitro culturing of the Star of Bethlehem in South Africa, comprising the following steps:

[0043] 1) Pretreatment of scales: select healthy and plump bulbs from the Star of Bethlehem in South Africa, select clean and complete scales inside, wash the scales and treat them with ethanol with a mass fraction of 75% for 45 seconds, and rinse off the ethanol with sterile water , and then sterilized with 0.15% mercuric chloride aqueous solution for 10 minutes, and then rinsed with sterile water for 6 times;

[0044] 2) Induction culture: cut the scales treated in step 1) into small squares of 2 cm × 4 cm, then inoculate them on the induction medium, and conduct induction culture for 45 days under light conditions, the culture temperature is 27 ° C, and the light duration is 10 h. d -1 , light intensity 1000lx, to obtain adventitious buds; the induction medium consists of: MS medium, kinetin 0.8mg / L, naphthaleneacetic acid 0.6mg / L, 6-benzylaminoadenine 2.0mg / L...

Embodiment 3

[0051] A method for in vitro culturing of the Star of Bethlehem in South Africa, comprising the following steps:

[0052] 1) Pretreatment of scales: select healthy and plump bulbs from the Star of Bethlehem in South Africa, pick out clean and complete scales inside, soak the scales in water with 2 drops of detergent for 4 minutes, wash them with clean water, and use quality Treat with 75% ethanol for 60 seconds, rinse off the ethanol with sterile water, then sterilize with 0.15% mercuric chloride aqueous solution for 15 minutes, and then rinse with sterile water for 6 times;

[0053] 2) Induction culture: Cut the scales treated in step 1) into small squares of 3 cm × 4 cm, then inoculate them on the induction medium, and conduct induction culture for 42 days under light conditions, the culture temperature is 26 ° C, and the light duration is 10 h. d -1 , light intensity 900lx, to obtain adventitious buds; the induction medium consists of: MS medium, kinetin 0.6mg / L, naphthale...

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Abstract

The invention provides an Ornithogalum Sauderside isolated culture method. The Ornithogalum Sauderside isolated culture method includes a scale explants pretreatment process, an adventitious bud induction culture process, an adventitious bud multiplication culture process and an adventitious bud rooting culture process. The Ornithogalum Sauderside isolated culture method succeeds in solving the problems of easiness in rotting, low reproduction coefficient, long reproduction cycle and failure to obtain sufficient high-quality Ornithogalum Sauderside seedlings in bulblet propagation, has the advantages of high Ornithogalum Sauderside seedling reproduction coefficient, short culture period and capability of culturing all year round, can provide sufficient high-quality seedlings for farmer households and enhance production benefits for the farmer households and lays the firm foundation for large-scale popularization and planting of Ornithogalum Sauderside.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a South African Star of Bethlehem in vitro culture method. Background technique [0002] Star of Bethlehem (Ornithogalum Sauderside) is a perennial bulbous flower native to South Africa, commonly known as velvet in the flower market. Because of its slender and hard flower stems, long-lasting flowering period, white petals and elegant shape, it is widely used in various celebrations and festivals, and is very popular among consumers. Since 2007, three flower enterprises in Yunnan, Qianhui, Lianhe, and Caichen introduced a small amount of seeds and bulbs for trial planting and succeeded. Due to the advantages of simple cultivation, low cost, quick results, and significant benefits, it has been in Yuanjiang, Yunnan Province in recent years. The county carries out bulb multiplication and cut flower planting. At present, almost all the South African Star of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 桂明春梁国平李玲田海管艳孙小龙唐敏
Owner YUNNAN INST OF TROPICAL CROPS
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