A functional vegetable seedling-raising biological matrix containing Bacillus subtilis g10 and its preparation method

A Bacillus subtilis and seedling raising biological substrate technology, applied in the field of agricultural microorganisms, can solve the problems of insufficient systematic and in-depth research, no large-scale promotion products, etc., achieving good growth promotion effect, obvious technical and functional advantages, and efficient growth promotion. effect of ability

Active Publication Date: 2017-04-26
甘肃丝路盛丰生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some domestic experts are trying to work in this area, mainly focusing on using AM mycorrhiza to inoculate existing substrates for seedling cultivation. Although some results have been achieved, the research is not systematic and in-depth, nor has it formed a large-scale promotion and has been recognized by the majority of farmers. products, so the research on this patent becomes particularly important

Method used

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  • A functional vegetable seedling-raising biological matrix containing Bacillus subtilis g10 and its preparation method
  • A functional vegetable seedling-raising biological matrix containing Bacillus subtilis g10 and its preparation method
  • A functional vegetable seedling-raising biological matrix containing Bacillus subtilis g10 and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, isolation and identification of bacterial strain

[0035] Take the root of pepper, cut it into small pieces about 3cm long, take 10g of plant root in a triangular flask filled with 90ml of sterile water, shake it in a shaker at 30°C and 170r / min for 20min, and make a soil suspension, 80°C After 20 minutes in water bath, dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5After waiting for different gradient dilutions, smear the plate, culture in the dark for 1d to 2d in the incubator, and then pick a single colony, after plate purification, a total of 150 strains were selected, and the auxin indoleacetic acid (IAA) produced by each strain was determined respectively (method References: Glickmann E, Dessaux Y. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. [J]. Applied and environmental microbiology, 1995, 612) and the ability to produce ACC deaminase (method reference: Glick BR. Mo...

Embodiment 2

[0038] The production of embodiment 2 functional bacterium bacterial liquid

[0039] Inoculate strain G10 (CGMCC NO.9921) into PDA culture medium for liquid fermentation production. The conditions for fermentation production are: pH 7.0, fermentation temperature 30°C, stirring speed 170rpm / min, spores are formed in the middle and late stages of fermentation, and the fermentation time is 48h, so that the amount of bacteria or spores in the fermentation broth is ≥1×10 10 a / ml;

[0040] The preparation method of the PDA medium used is as follows, taking the preparation of 1L medium as an example: 200g of potatoes are peeled, cut into small pieces, put into water and boiled, boiled for 30min and then filtered, add 20g of ordinary sucrose to the filtrate and make it to 1000ml , natural pH, sterilized at 121°C for 20min.

Embodiment 3

[0041] Example 3, research and development of functional biological matrix

[0042] 3.1 Effect of biological matrix seedling cultivation with strain G10 as functional bacteria

[0043] Research and development of the substrate: the strain G10 was connected to the common seedling cultivation substrate and mixed evenly. Selective medium for functional strain G10 in the matrix: 10.0g peptone, 5.0g yeast powder, 10.0g NaCl, 2.0% agar, 1000mL deionized water, pH 7.2‐7.4, autoclaved at 121°C for 20min; 1% polymyxa Cycloheximide 2mL, 1% cycloheximide 4mL (antibiotics are added after the medium is cooled before being poured). The number of colonies of functional bacteria G10 is calculated by dry weight per gram of substrate. After counting, the number of functional bacteria in the biological matrix is ​​about 6.73×10 7 CFU / g.

[0044] The treatments for the seedling raising experiment were as follows: 1) Common seedling raising medium (CK2); 2) Seedling raising medium (CK1) added ...

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Abstract

The invention discloses a functional vegetable seedling-raising biological matrix containing Bacillus subtilis G10 and a preparation method thereof. A strain of Bacillus subtilis (Bacillus subtilis) G10 was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, with the preservation date being November 3, 2014, and the preservation number is CGMCC NO.9921. A functional vegetable seedling-raising biological substrate is obtained by adding the Bacillus subtilis G10 fermentation liquid of the present invention to a common seedling-raising substrate. The use of this functional biological substrate for vegetable seedlings has a good effect on promoting the growth of vegetables and fruits at the seedling stage, and high-quality seedlings can be obtained. growth.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a functional vegetable seedling-raising biological matrix containing Bacillus subtilis G10 and a preparation method thereof. Background technique [0002] With the development of modern agriculture in our country, more and more people pay more and more attention to industrial vegetable seedling cultivation, and plug seedling cultivation is the main measure of current industrial seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, in China, materials rich in organic matter (such as peat, decomposed plant residues, etc.) are mainly used, and an appropriate proportion of light inorganic materials, such as vermiculite and expanded perlite, are used to make see...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/125
CPCC05F11/08C05G3/00C12N1/20C12N1/205C12R2001/125
Inventor 沈其荣张杨李荣文春燕高琦赵买琼
Owner 甘肃丝路盛丰生物科技集团有限公司
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