A strain containing Bacillus dead valley and its prepared functional vegetable seedling biological matrix

A technology of Bacillus dead valley and biological substrate for seedling cultivation, which is applied in the field of agricultural microorganisms, can solve the problems of slow development of biological substrate for seedling cultivation, and achieve the effects of good growth promotion effect, obvious functional advantages, and obvious technical advantages

Active Publication Date: 2018-05-01
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of slow development of domestic seedling-raising biological substrates at present, and provide a kind of Bacillus dead valley for preparing functional vegetable seedling-raising biological substrates

Method used

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  • A strain containing Bacillus dead valley and its prepared functional vegetable seedling biological matrix
  • A strain containing Bacillus dead valley and its prepared functional vegetable seedling biological matrix
  • A strain containing Bacillus dead valley and its prepared functional vegetable seedling biological matrix

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Experimental program
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Effect test

Embodiment 1

[0035] Isolation and identification of embodiment 1 bacterial strain

[0036] Take the root of watermelon, cut it into small pieces about 3cm in length, take 10g of the plant root, put it in a triangular flask filled with 90ml of sterile water, shake it in a shaker at 30°C and 170r / min for 20min, and make a soil suspension, 80°C After 20 minutes in water bath, dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5After waiting for different gradient dilutions, smear LB plate, culture in the dark for 1 to 2 days in the incubator, and then pick a single colony, after plate purification, a total of 126 pure bacteria were selected, and the auxin indole acetic acid produced by each strain was determined respectively. IAA) (method reference: Glickmann E, Dessaux Y.A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. [J]. Applied and environmental microbiology, 1995, 612) and the ability to produce ACC deaminase (meth...

Embodiment 2

[0039] The production of embodiment 2, N23 fermented liquid

[0040] Inoculate strain N23 (CGMCC NO.9920) into PDA culture medium for liquid fermentation production. The conditions for fermentation production are: pH 6.5, fermentation temperature range 30°C, stirring speed 170 rpm, spores are formed in the middle and late stages of fermentation, and fermentation The time is 48 hours, so that the amount of bacteria or spores in the fermentation broth is ≥1×10 10 a / ml;

[0041] The preparation method of the PDA medium used is as follows, taking the preparation of 1L medium as an example: 200g of potatoes are peeled, cut into small pieces, put into water and boiled, boiled for 30min and then filtered, add 20g of ordinary sucrose to the filtrate and make it to 1000ml , natural pH, sterilized at 121°C for 20min.

Embodiment 3

[0042] Example 3, research and development of functional biological matrix

[0043] 3.1 The effect of biological matrix seedlings with strain N23 as functional bacteria

[0044] Research and development of the substrate: the strain N23 was inoculated (according to 5% of the dry weight of the substrate, the same below) into the common seedling cultivation substrate, and mixed evenly. The count of functional strain N23 in the matrix adopts selective medium: 10.0g peptone, 5.0g yeast powder, 10.0g NaCl, 2.0% agar, 1000mL deionized water, pH 7.2-7.4, autoclaved at 121℃ for 20min; more than 1% Colistin 2mL, 1% cycloheximide 4mL (antibiotics are added after the medium is cooled before being poured): the number of colonies of functional bacteria N23 is calculated by dry weight per gram of substrate. After counting, the number of functional bacteria in the biological matrix is ​​about 3.35×10 7 CFU / g.

[0045] The seedling raising test is processed as follows: 1) common seedling ra...

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Abstract

The invention discloses a strain containing bacillus dead valley and the functional vegetable seedling-raising biological substrate prepared therefrom. A Bacillus dead valley N23 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.9920. The functional vegetable seedling-raising biological matrix is ​​obtained by adding functional bacterial strain N23 to common seedling-raising matrix. This product is aimed at cultivating seedlings of watermelon, pepper, tomato, cucumber and other crops. Select a specific Bacillus dead valley N23, and add it to the matrix to obtain an active biological matrix product. Using this product, it can be used for watermelon, pepper, tomato, etc. , the growth-promoting effect of cucumber seedlings is good, and high-quality seedlings with a large number of active functional microorganisms in the rhizosphere can be bred. It can also improve the yield of such crops in field planting.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a functional vegetable seedling-raising biological substrate containing Bacillus dead valley and the prepared functional matrix. Background technique [0002] With the development of modern agriculture in our country, more and more people pay more and more attention to industrial vegetable seedling cultivation, and plug seedling cultivation is the main measure of current industrial seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, in China, materials rich in organic matter (such as peat, decomposed plant residues, etc.) are mainly used, and an appropriate proportion of light inorganic materials, such as vermiculite and expanded perlite, are used to make...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/07
CPCC05F11/08C12N1/205C12R2001/07
Inventor 沈其荣李荣张杨文春燕高琦赵买琼
Owner NANJING AGRICULTURAL UNIVERSITY
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