A kit for culturing liver-derived stem cells

Abstract

The invention provides a kit used for culturing liver-derived stem cells. The kit is internally provided with a plurality of reagent bottles; the plurality of reagent bottles are respectively filled with a nutrient solution, a medium additive, a growth factor I, a growth factor II, a digestive solution, a cleaning solution, a frozen stock solution and a cell seed. The kit provided by the invention can be used for culture, amplification and cryopreservation of liver-derived stem cells, overcomes the problems of low liver-derived stem cell culturing efficiency, great difference among culture batches, unsatisfactory recovery rate after cryopreservation and the like, realizes high-efficiency amplification of liver-derived stem cells in an ideal nutrient balance state, substantially reduces time for culture and amplification, is applicable to culture of liver-derived stem cells, and provides abundant sources for further experimental studies and medical treatment of liver-derived stem cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a kit for cultivating liver-derived stem cells. Background technique [0002] Existing technologies have shown that hepatic stem cells exist in the mature liver and have a strong regenerative capacity. Hepatic stem cells are a kind of stem cells with self-renewal ability and multi-directional differentiation potential. They can produce stem cells with the same phenotype and genotype as themselves through their own symmetric and asymmetric divisions, and can also produce stem cells in different developmental stages and Cells with different differentiation directions, such as hepatocytes, bile duct epithelial cells and pancreatic epithelial cells, can replace hepatocyte transplantation and liver transplantation as an important cell source for the treatment of organ failure. Both have great potential. In the prior art, there has been a precedent of using the liver tissue of aborted fe...

AI Technical Summary

Problems solved by technology

If the reagents and methods used for cultivating and preserving hepatic stem cells selected by the experimenters are not appropriate, the quantity and quality of the cultured hepatic stem cells will often fail to meet the requirements, wasting manpower and other resources. The kit for massively expanding and culturing hepatic stem cells is of great significance

At present, there are few kits specially used for culturing liver stem cells. Some kits for cultivating other stem cells are disclosed in the prior art. For example, CN104928238A discloses a kit for preparing adipose-derived mesenchymal stem cells, including liquid, fat washing liquid, lipolysis liquid, cell washing liquid, cell culture liquid, cell digestion liquid, and cell cryopreservation liquid, but the effect of this kit for culturing and expanding stem cells is not ideal, and the safety and stability of the reagents are not high. Issues such as low cell viability and proliferation

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