Method for determination of organic acids and flavone components in polygonum viviparum

A determination method and technology of Polygonum vulgaris, which are applied in the directions of measuring device, material separation, analysis of materials, etc., can solve the problems of not fully reflecting the quality of medicinal materials, unfavorable quality of Polygonum vulgaris, etc., and achieve increased ionization effect, good degree of separation, and simple steps. Effect

Active Publication Date: 2017-03-22
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Polygonum viburnum is recorded in the "Ministry of Health Drug Standard Tibetan Medicine of the People's Republic of China (Volume I)", in which there is only microscopic identification of medicinal materials and no content determination indicators, which is not conducive to effective control of the quality of Polygonum viburnum
At present, there are "Simultaneous Determination of Vitexin, Quercetin and Quercetin Contents in Tibetan Medicine Polygonum vermilion by RP-HPLC Method" reported by Xu Yanli et al. and "Simultaneous Determination of Gallic Acid, Chlorophyll The content of ortho acid and quercetin components" Quantitative research on the organic acids and flavonoids in Polygonum vermicelli can only be measured for three substances, which cannot fully reflect the quality of the medicinal materials, and it is impossible to comprehensively analyze the medicinal materials of Polygonum vermilion and overall rating

Method used

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  • Method for determination of organic acids and flavone components in polygonum viviparum
  • Method for determination of organic acids and flavone components in polygonum viviparum
  • Method for determination of organic acids and flavone components in polygonum viviparum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Reagents, medicinal materials, standards and instruments

[0047] 1.1 Reagents and medicinal materials

[0048]

[0049] 1.2 Standards

[0050]

[0051] 1.3 Instruments

[0052]

[0053] 2. Preparation of the test solution

[0054] Accurately weigh 1.0g of Polygonum versicolor powder passed through a No. 3 sieve, place it in a stoppered Erlenmeyer flask, accurately measure 30mL of 50% methanol, weigh it, place it in an ultrasonic instrument at 55°C for 15 minutes, let it cool, and weigh it , Use methanol to make up the lost weight, shake well, filter, and then filter through a 0.22μm filter membrane, and take the continued filtrate, that is.

[0055] 3. Liquid chromatography and mass spectrometry conditions of LC-MS / MS

[0056] 3.1 Liquid chromatography conditions for LC-MS / MS

[0057] Agilent ZORBAX SB-Aq chromatographic column (150mm×4.6mm, 5μm), mobile phase 0.1% aqueous formic acid (A)-acetonitrile (B), gradient elution according to Table 1, flow rate...

Embodiment 2

[0068] Example 2 Quantitative analysis of gallic acid and chlorogenic acid

[0069] On the basis of Example 1, gallic acid and chlorogenic acid can also be quantitatively analyzed.

[0070] 1. Preparation of reference solution:

[0071] Mixed reference substance: take an appropriate amount of gallic acid and chlorogenic acid reference substance, weigh them accurately, add 50% methanol solution to make a mixed reference substance solution containing 0.086mg of gallic acid and 0.173mg of chlorogenic acid per 1mL, and obtain it;

[0072] Gallic acid reference substance solution: take an appropriate amount of gallic acid, accurately weighed, add 50% methanol solution to make a reference substance solution containing 0.0216mg of gallic acid per 1mL;

[0073] Chlorogenic acid reference substance solution: remove an appropriate amount of chlorogenic acid, accurately weigh it, add 50% methanol solution to make a reference substance solution containing 0.945 mg of chlorogenic acid per...

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Abstract

The invention discloses a method for simultaneous determination of organic acids and flavone components in polygonum viviparum, wherein the method comprises the following steps: (1) preparing a test sample solution; (2) detecting with LC-MS/MS; and (3) carrying out component qualitative analysis. On the basis of qualitative analysis of the components of the polygonum viviparum, a reference substance solution is prepared, and the contents of chlorogenic acid and gallic acid in the polygonum viviparum are also determined. 10 kinds of substances in the two active components of the organic acids and the flavones in the polygonum viviparum are determined at the same time for the first time, and a quality control method of the polygonum viviparum medicinal material is improved. The LC-MS/MS analysis detection method is adopted to be combined with a multi-wavelength switching procedure, and the method has the advantages of being simple, stable, fast, high in efficiency, high in sensitivity, good in repeatability and low in interference, can complete the detection of 10 kinds of substances within 1 h, and obtains a chromatogram which can contain multiple pieces of wavelength information at the same time.

Description

[0001] field of invention [0002] The invention belongs to the technical field of detecting the quality of Tibetan medicinal materials by means of modern analysis and detection means, and more specifically relates to a method for measuring organic acids and flavonoids in Polygonum viburnum by using multi-wavelength switching. Background technique [0003] Polygonum viviparum is the dried rhizome of Polygonum Viviparum L, which is a plant in the Polygonaceae family. It is recorded in "Jingzhu Materia Medica" that Ranbu (Polygonum viviparum) is sweet, astringent, and sour, and has the effect of relieving diarrhea and relieving cold and pain in the intestines. It can be used to treat wind-heat, eliminate epidemic heat, harmonize and harmonize disorders, treat senile diseases, rheumatism, reduce fever, regulate menstruation, astringe, stop bleeding, etc. "Chinese Tibetan Medicine" records that Polygonum versicolor mainly contains anthraquinone, tannins, polysaccharides, flavonoid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 廖娜钱正明艾中李文佳陶盛昌龚欢
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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