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Separation method of collagen antifreeze peptide

A separation method and collagen technology, applied in the field of bioengineering, can solve the problems of difficult to achieve rapid, high-throughput screening, cumbersome and time-consuming screening process, and achieve the effects of low cost and simple separation process

Active Publication Date: 2020-11-27
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The entire screening process is tedious and time-consuming, making it difficult to achieve rapid and high-throughput screening

Method used

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  • Separation method of collagen antifreeze peptide
  • Separation method of collagen antifreeze peptide
  • Separation method of collagen antifreeze peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of collagen antifreeze peptide, specifically comprising the steps of:

[0031] (1), preparation of collagen

[0032] ①. Use scissors to remove the hair on the surface of the pigskin, wash it with distilled water, and store it at -20°C for later use.

[0033] ②. Cut the pigskin after ① into pieces (0.5mm×0.5mm), degrease with 10% butanol solution prepared with 10 times the volume of double distilled water for 24 hours, change the butanol solution every 12 hours, and then wash it with distilled water .

[0034] ③. Soak degreased pigskin in 0.5M acetic acid solution prepared with double distilled water for 12 hours, and homogenate with 10 times the volume of the same solution; add (w / v) pepsin dissolved in 1% double distilled water to the homogenate (1200U / g), hydrolyzed at 4°C for 48h, centrifuged (5000g, 40min) after hydrolysis, and collected the supernatant; adding NaCl powder to the supernatant for salting out (to make the final concentration of ...

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Abstract

Disclosed is a separating method for collagen antifreeze peptide. The separating method mainly comprises three steps of collagen preparing, collagen enzymolysis compound preparing and collagen antifreeze peptide separating, and finally the collagen antifreeze peptide with high antifreeze activity is obtained. The amino acid complete sequence of the collagen antifreeze peptide is Leu-Gly-Lue-Gly-Pro-Leu-Gly-Arg-Gly-Pro-Leu-Leu. According to the separating method, the separating process is simple, cost is low, and scale production is easy to achieve.

Description

technical field [0001] The invention relates to bioengineering, in particular to a method for separating collagen antifreeze peptides. Background technique [0002] The sources of food-derived antifreeze peptides mainly include extraction from natural foods, preparation of enzymatically hydrolyzed food proteins, etc. Among them, enzymatic hydrolysis of protein to prepare antifreeze peptide has the advantages of mild preparation conditions, simple process, and easy control of the process, and has become the main method for current research and actual production. However, the peptide components obtained by enzymatic hydrolysis are very complex, including various inactive peptides, target peptides and a large amount of salt, so they need to be screened to varying degrees according to different research and application purposes. The current conventional method is to use a variety of chromatographic techniques. Each step of chromatographic separation divides the enzymatic hydrol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/30C07K1/22
Inventor 赵莹曹慧徐斐于劲松袁敏
Owner UNIV OF SHANGHAI FOR SCI & TECH
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