A magnetic core-shell nanoparticle and its preparation method and application
A magnetic nanoparticle and nanoparticle technology, applied in the field of glycosylated proteins, to achieve the effects of increasing graft density, effective separation and enrichment, and simple operation
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Embodiment 1
[0048] Preparation of dipeptide functionalized polymers
[0049] The preparation steps of dipeptide (L-Asp-L-Phe) functionalized polymer are as follows: 1 mol of methyl esterified dipeptide L-Asp-L-Phe is dissolved in chloroform, and triethylamine is added dropwise to alkalinity, in In an ice-water bath, 1.2-1.5 mol of chloroacetyl chloride was added dropwise and reacted for 12 hours to obtain a chloroacetylated dipeptide. Dissolve 1 g of chloroacetylated dipeptide, polyethyleneimine (PEI, with a degree of polymerization ranging from 5 to 200,000), potassium carbonate, and sodium iodide in 100 mL of anhydrous N,N-dimethylformamide solution, After 24 hours of reaction in the dark at room temperature under the protection of nitrogen, the dipeptide functionalized polymer was obtained by purification and dialysis.
[0050] The following is a schematic diagram of the preparation process of the dipeptide functionalized polymer:
[0051]
Embodiment 2
[0053] Porous silica-coated magnetic nanoparticles (Fe 3 o 4 @SiO 2 @mSiO 2 Indicates the preparation of
[0054] The magnetic nanoparticles of the present embodiment select Fe 3 o 4 , it can be understood that other types of magnetic nanoparticles are also suitable for the present invention.
[0055] 1) Preparation of Fe 3 o 4 Magnetic nanoparticles: 0.3M ferric chloride hexahydrate and 0.2M ferrous sulfate heptahydrate in aqueous solution (H 2 O) fully dissolved in, under the protection of nitrogen, add the ammoniacal liquor (NH 3 ·H 2 O), and fully stirred at a temperature of 85 ° C. After 2 hours, the magnetic nanoparticles (Fe 3 o 4 ) were separated and washed thoroughly with ethanol;
[0056] 2) Preparation of Fe 3 o 4 @SiO 2 : the Fe 3 o 4 Magnetic nanoparticles are dispersed in a mixed solution of ethanol, deionized water, ammonia water and orthosilicate (80:17:2.5:0.5 by volume), and the obtained 2g / L magnetic nanoparticles are fully stirred at room t...
Embodiment 3
[0059] Preparation of magnetic core-shell nanoparticles
[0060] 1) Magnetic nanoparticles (Fe 3 o 4 @SiO 2 @mSiO 2 ) 0.1g dispersed in 100mL toluene, adding siloxane isothiocyanate modifier accounting for 5% of the volume ratio of the aforementioned solution, reflux reaction at 85°C for 24 hours, and washing with ethanol three times;
[0061] 2) Mix and disperse the dipeptide functionalized polymer obtained in Example 1 and the siloxane-modified magnetic nanoparticles obtained in the above step 1) in 100mL N,N-dimethylformamide solution, and add triethyl amine to alkaline, and reacted for 24 hours, centrifuged and washed with ethanol three times to obtain magnetic core-shell structure nanoparticles.
[0062] figure 1 Schematic diagram of the structure of the magnetic core-shell nanoparticle. The magnetic core-shell structure nanoparticles include a plurality of magnetic nanoparticles 1, a solid silica shell 2 covering a plurality of magnetic nanoparticles 1, a porous si...
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