Methods and compositions for determining blood levels of pod protein

Abstract

In one aspect, disclosed herein is a homogeneous assay method for determining blood levels of a pod protein molecule (asparaginyl endopeptidase). In one aspect, the assay utilizes nanoparticles of a specific size coated with one or more antibodies specific for the podin molecule or one or more fragments thereof. In one aspect, the assay is designed in a homogeneous manner to have a dynamic range of 0.2 ng / mL to 160 ng / mL. In another aspect, disclosed herein is a kit for determining blood levels of podin, said kit comprising two parts of reagents (R1 and R2), said kit being suitable for use on a clinical chemistry analyzer use. In one aspect, the cutoff value for distinguishing normal risk from high risk for cancers such as breast cancer, colorectal cancer, and gastric cancer is 18 ng / mL ± 3 ng / mL.

Description

technical field [0001] In certain aspects, the present disclosure relates to methods, kits, and devices for analyzing biomolecules. In particular aspects, the present disclosure relates to methods, kits, and devices for determining the concentration of legumain in a sample. In some embodiments, the concentration of legume protein in a blood sample is determined by a microparticle-enhanced immunoassay, such as a latex agglutination immunoassay. Background technique [0002] Pod protein is a protein encoded by the LGMN gene in humans (Chen J.M. et al., J. Biol. Chem. 1997; Tanaka T. et al., Cytogenet. Cell Genet. 1966; Halfon S. et al., FEBS Lett. 1998). This gene encodes a cysteine ​​protease with strict specificity for the hydrolysis of asparaginyl bonds, cleaving peptide bonds with Asn or Asp at the P1 position (Schwarz G. et al., J. Biol. Chem. 2002) . Several alternatively spliced ​​transcript variants have been described, but the biological availability of only two ha...

AI Technical Summary

Problems solved by technology

Although pod protein as a target is attractive for the advancement of anticancer drug design and drug research on this target, there is a lack of an automated way to measure the blood level of pod protein by clinical chemistry analyzer or automated immunoassay analyzer clinical diagnostic methods

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