The invention discloses application of knockout of a Glyma. 12G128600
gene to improvement of
insect resistance of soybean prodenia litura, and relates to the technical field of
biology. According to the invention, the soybean Glyma. 12G128600
gene is knocked out, so that the improvement on multiple characters of the soybean is realized. Soybean plants with the
gene knocked out show remarkable
insect resistance to prodenia litura, the weight of prodenia litura larvae can be remarkably reduced, and the
biting degree of the plants is relieved. Meanwhile, the
gene knockout can significantly improve the pod number and the segment number of the soybean, optimizes the pod bearing and branching characteristics of the
plant, and lays an agricultural foundation for soybean yield increase; the
gene knockout can also effectively reduce the
plant height of the soybean, optimize the
plant type structure, improve the lodging resistance of the plant and adapt to close planting cultivation in the field. The invention provides important
technical support for soybean
insect-resistant breeding and high-yield and high-quality variety cultivation.