Preparation method and application of a biomimetic cell nanocapsule carrier

A technology of nanocapsules and cells, which is applied in the fields of chemical industry and medicine, can solve the problems of short half-life of small molecule drugs, drug resistance, low selectivity of tumor cells, etc., and achieve improved bioavailability, good biocompatibility, immune low effect

Active Publication Date: 2019-02-22
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the half-life of small molecule drugs is short, and frequent administration is required in order to achieve the therapeutic effect, and the selectivity of small molecule drugs to tumor cells is low. While damaging tumor cells, normal tissue cells are also damaged to a certain extent.
In addition, during the course of chemotherapy, tumor cells will also develop drug resistance, resulting in a greatly reduced therapeutic effect

Method used

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  • Preparation method and application of a biomimetic cell nanocapsule carrier

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Experimental program
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Effect test

Embodiment 1

[0013] When the present invention is implemented, it is realized by the following steps:

[0014] (1) Mammary gland cell culture: Commercially available human normal breast cells (Hs578Bst, purchased from Beijing Beina Chuanglian Biotechnology Research Institute) were incubated at a temperature of 37°C and a gas volume concentration of 5% CO 2 , placed in the culture medium under saturated humidity, the medium used was RPMI Medium1640 medium containing 10% fetal bovine serum and 1% penicillin and streptomycin, and the cells were subcultured once every 3 days, specifically The steps of cell subculture are as follows: ① Discard the original culture medium of the human normal breast cells purchased in the cell culture flask, wash with 5ml pH6.5 PBS buffer three times each time; ② Add 2ml trypsin and incubate at 37℃ Put it in the box for 1.5min, and then observe it under an inverted microscope. When the cells shrink and become round, immediately add 5ml of medium; ④Put the blown ...

Embodiment 2

[0019] When the present invention is implemented, it is realized by the following steps:

[0020] (1) Mammary gland cell culture: Commercially available human normal breast cells (Hs578Bst, purchased from Beijing Beina Chuanglian Biotechnology Research Institute) were incubated at a temperature of 37°C and a gas volume concentration of 5% CO 2 , placed in the culture medium under saturated humidity, the medium used was RPMI Medium1640 medium containing 10% fetal bovine serum and 1% penicillin and streptomycin, and the cells were subcultured once every 3 days, specifically The steps of cell subculture are as follows: ①Discard the original culture medium of the human normal breast cells purchased in the cell culture flask, wash with 5ml pH7.5 PBS buffer three times each time; ②Add 2ml trypsin and culture at 37℃ Put it in the box for 2 minutes, and then observe it under an inverted microscope. When the cells shrink and become round, immediately add 5ml of medium; Regular pipetti...

Embodiment 3

[0025] When the present invention is implemented, it is realized by the following steps:

[0026] (1) Mammary gland cell culture: Commercially available human normal breast cells (Hs578Bst, purchased from Beijing Beina Chuanglian Biotechnology Research Institute) were incubated at a temperature of 37°C and a gas volume concentration of 5% CO 2, placed in the culture medium under saturated humidity, the medium used was RPMI Medium1640 medium containing 10% fetal bovine serum and 1% penicillin and streptomycin, and the cells were subcultured once every 3 days, specifically The steps of cell subculture are as follows: ①Discard the original culture medium of the human normal breast cells purchased in the cell culture flask, wash with 5ml pH7.0 PBS buffer three times each time; ②Add 2ml trypsin and culture at 37℃ Put it in the box for 2 minutes, and then observe it under an inverted microscope. When the cells shrink and become round, immediately add 5ml of medium; Regular pipettin...

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Abstract

The invention relates to a preparation method and application of a bionic cell nanocapsule carrier and may effectively solves the problems of tumor drugs. According to the technical scheme, the bionic cell nanocapsule carrier is prepared by: culturing commercially available human normal breast cells, centrifuging 200-250 ml of subculture liquid, removing supernate, adding 1.5-3.5 ml of normal saline, mixing well, filtering, adding 80-120Mul of RGD-lysine-(octadecanoic acid) 2 solution having mass concentration of 5 mg / ml, mixing well, incubating at 37 DEG C for 2-4 h, and dialyzing with PBS (phosphate buffer solution) to remove free RGD-lysine-(octadecanoic acid) 2. The bionic cell nanocapsule carrier prepared herein combines the advantages in both cells and nano carriers, is simple to prepare and low in immune effect, has good biocompatibility, good degradability, long cycle characteristic, and good targeting property, and provides improved bioavailability of drugs.

Description

technical field [0001] The invention relates to the fields of chemical industry and medicine, in particular to a preparation method and application of a bionic cell nanocapsule carrier. Background technique [0002] Malignant tumors are common and frequently-occurring diseases that seriously threaten human health. Currently, the three main treatments for malignant tumors are surgery, chemotherapy and radiotherapy. The application of cytotoxic antineoplastic drugs for chemotherapy is still an important means of comprehensive treatment of tumors. However, small-molecule drugs have a short half-life, so they need to be administered frequently in order to achieve therapeutic effects, and small-molecule drugs have low selectivity for tumor cells. While damaging tumor cells, they also damage normal tissue cells to a certain extent. In addition, tumor cells will also develop drug resistance during chemotherapy, resulting in a greatly reduced therapeutic effect. [0003] In view ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K9/50A61K47/46A61K47/18A61K31/404A61P35/00
CPCA61K9/0002A61K9/5015A61K9/5068A61K9/5089A61K31/404
Inventor 张振中张俊利史进进张红岭岳娅乐吴思璇
Owner ZHENGZHOU UNIV
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