Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Variovorax paradoxus DEA-3 and immobilization and application thereof

A technology of immobilizing small balls and phages, which is applied in the field of environmental microorganisms, can solve problems such as entering the environment and water pollution, and achieve the effects of low production cost, good degradation effect, and convenient use

Inactive Publication Date: 2017-04-26
JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since dimethylethanolamine is miscible with water, a large amount of dimethylethanolamine will enter the waste water and enter the environment during use, causing water pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Variovorax paradoxus DEA-3 and immobilization and application thereof
  • Variovorax paradoxus DEA-3 and immobilization and application thereof
  • Variovorax paradoxus DEA-3 and immobilization and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Isolation and Identification of Debate Phage DEA-3

[0024] The controversial phage DEA-3 was isolated and screened from the activated sludge pool of the sewage treatment station of the fine chemical plant.

[0025] The specific process of screening is:

[0026] 1) Take 5ml of activated sludge from the sewage treatment station of a fine chemical plant and inoculate it into an inorganic salt medium containing 200mg / L N,N-dimethylethanolamine. The formula is: 200mg / LN,N-dimethylethanolamine, 1.5 g / L K2HPO4, 0.5g / L KH2PO4, 0.2g / L MgSO4 7H2O, 1.0g / L NaCl, cultivated in a shaker incubator at 30°C-37°C for 7 days, then removed 5m of the mixed solution and inoculated to contain 400mg / LN,N - In the inorganic salt medium of dimethylethanolamine, the formula is: 400mg / L N,N-dimethylethanolamine, 1.5g / L K2HPO4, 0.5g / L KH2PO4, 0.2g / L MgSO4 7H2O, 1.0g / L NaCl, the enriched solution was obtained after culturing for 7 days;

[0027] 2) Take 1.0ml of N,N-dimethylethanolamin...

Embodiment 2

[0030] Example 2 The situation that the free argumentative phage DEA-3 can grow with N,N-dimethylethanolamine as the only carbon source and carbon source

[0031] Pick a single bacterium colony from the plate that grows Vulgarophilus DEA-3, inoculate it in LB liquid medium, cultivate it in 30℃, 160rpm shaker for 12h (OD600≈1.0), centrifuge at 8000-12000rpm, discard Remove the supernatant, wash three times with sterile water, and resuspend in an equal volume of sterile water as the seed solution.

[0032]Fill a 250mL Erlenmeyer flask with inorganic salt medium containing 200mg / L p-N,N-dimethylethanolamine, the formulation of which is: 200mg / LN,N-dimethylethanolamine, 1.5g / L K2HPO4, 0.5g / L L KH2PO4, 0.2g / L MgSO4 7H2O, 1.0g / L NaCl, add PTA-2 seed solution according to the inoculum amount of 1% (volume fraction), cultivate in a shaker at 30°C and 160rpm, take out 4mL every 2h For the culture medium, the sample was centrifuged at 8000rpm and the supernatant was discarded, resuspen...

Embodiment 3

[0033] Example 3 The method of preparing the immobilized pellets from the controversial phage DEA-3

[0034] 1) Use an inoculation loop to pick a small amount of bacterial strains on the slant medium and inoculate them in LB liquid medium at 30°C and 160r / min for shaking culture to the logarithmic phase;

[0035] 2) Centrifuge the above cultured bacterial solution at 10000r / min for 3min, discard the supernatant, add the same volume of sterile water, shake well and centrifuge at 10000r / min for 3min, wash twice in this way, and use the same volume Suspended in sterile water to make a bacterial fluid.

[0036] 3) Weigh 0.8g sodium alginate and dissolve it in 20ml distilled water, add 1000mg / L bamboo charcoal, and ultrasonically disperse;

[0037] 4) The solution obtained in step 3 is dripped into 3% CaCl at room temperature with a syringe 2 The solution was cross-linked and calcified at 4°C for 6 hours to obtain calcium alginate gel-immobilized beads, which were washed twice wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses variovorax paradoxus DEA-3 which is classified and named as variovorax paradoxus DEA-3, the preservation date is February 9, 2015, the full name of the preservation institution is China General Microbiological Culture Collection Center (CGMCC), and the preservation accession number is CGMCCNO.10543. The variovorax paradoxus DEA-3 can grow by use of N, N-dimethyl ethanolamine as the sole carbon source and nitrogen source, the 200mg / L N, N-dimethyl ethanolamine degradation rate of the variovorax paradoxus DEA-3 can reach 94.5%; the 200mg / L N, N-dimethyl ethanolamine degradation rate of an immobilization small ball prepared by the variovorax paradoxus DEA-3 can reach more than 90%, the immobilization small ball prepared by the variovorax paradoxus has a very good degradation effect, and in addition, the immobilization small ball prepared by the variovorax paradoxus is low in production cost, convenient to use, and suitable for promotion and use in the treatment of wastewater containing the N, N-dimethyl ethanolamine.

Description

technical field [0001] The present invention relates to the technical field of environmental microorganisms, in particular to An Argumentative Phagocytosis DEA-3 and its immobilization and application . Background technique [0002] N,N-Dimethylethanolamine is a widely used organic intermediate, it is a colorless or light yellow liquid, it can be used to synthesize cation exchange resin, it can be used as a stabilizer for paint, and an additive for fuel oil , Anti-scaling agent for boilers, with the development of polyurethane industry, it can also be used as an important auxiliary agent for polyurethane processing. Since dimethylethanolamine is miscible with water, a large amount of dimethylethanolamine will enter the waste water and enter the environment during use, causing water pollution. [0003] Microbial degradation has the advantages of low cost, high efficiency, no secondary pollution, and good ecological restoration. The wastewater of ethanolamine is of grea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N11/10C12N11/14C12N1/205C12R2001/01
Inventor 董晓梦袁鹏张永乐周乃亮李珊珊
Owner JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com