Application of compound HUBIN-1 in preparation of drug for preventing and/or treating liver inflammatory diseases

A technology for HUBIN-1 and inflammatory diseases, applied in the field of drug research and development, can solve problems such as not being able to be used on a large scale, and achieve obvious social benefits

Inactive Publication Date: 2017-05-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although liver transplantation can be used for patients with acute liver failure due to some specific reasons, it cannot be used on a large scale clinically because of the source of organs and the cost of treatment

Method used

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  • Application of compound HUBIN-1 in preparation of drug for preventing and/or treating liver inflammatory diseases
  • Application of compound HUBIN-1 in preparation of drug for preventing and/or treating liver inflammatory diseases
  • Application of compound HUBIN-1 in preparation of drug for preventing and/or treating liver inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of compound HUBIN-1 solution

[0040] Compound HUBIN-1, the stock solution of compound HUBIN-1 prepared after DMSO solubilization, was diluted with PBS to prepare the solvent of compound HUBIN-1 before injection.

Embodiment 2

[0041] Example 2 The protective effect of compound HUBIN-1 on LPS / D-GalN-induced hepatitis in mice-dose-effect relationship study

[0042] Animal models are research tools to verify whether new therapeutic drugs and new treatment methods are effective. At present, the animal models for simulating severe hepatitis used in the world include drug-induced severe hepatitis models (paracetamol model, LPS / D-GalN model, etc.) , thioacetamide model, carbon tetrachloride model, etc.), acute liver ischemia model, partial hepatectomy model, etc. Among them, the LPS / D-GalN model is an ideal severe hepatitis animal model because of its good reproducibility, non-obvious extrahepatic toxicity, and clinical liver damage.

[0043] Six-week-old male C57BL / 6 mice (purchased from Nanjing Institute of Biomedicine) were randomly divided into normal control group, compound HUBIN-1 group, model group (LPS / D-GalN group), treatment group (0.625mg / kg HUBIN -1+LPS / D-GalN group, 1.25mg / kg HUBIN-1+LPS / D-Ga...

Embodiment 3

[0050] Example 3 Therapeutic effect of compound HUBIN-1 on LPS / D-GalN-induced hepatitis in mice-time-effect relationship study

[0051] Six-week-old male C57BL / 6 mice (purchased from Nanjing Institute of Biomedicine) were randomly divided into normal control group, model group (LPS / D-GalN group), treatment group (2.5mg / kg HUBIN-1+LPS / D -GalN group). The treatment group was intraperitoneally injected with LPS / D-GalN (10 μg / kg LPS + 400 mg / kg D-GalN), and administered 30 minutes before LPS / D-GalN modeling, 30 minutes, 1 hour, and 2 hours after modeling. The compound HUBIN-1 was injected intraperitoneally at a dose of 2.5 mg / kg; the model group was replaced with an equal volume of solvent for the compound HUBIN-1; the normal control group was not treated. Six hours after the LPS / D-GalN model was established, the blood was collected by removing the eyeball, and the liver tissue was fixed and frozen. Biochemical and pathological examinations were performed to evaluate the degree o...

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Abstract

The invention relates to the field of drug research and development and particularly discloses an application of a compound HUBIN-1 in preparation of a drug for preventing and/or treating liver inflammatory diseases. Extensive and deep research discovers the hepatocyte protection function and the anti-inflammatory treatment function of the compound HUBIN-1 for the first time, the compound HUBIN-1 is applicable to various types of liver inflammations, and the application field of the compound HUBIN-1 is broadened. The novel drug for preventing and/or treating various liver inflammatory diseases such as acute hepatitis, chronic hepatitis and severe hepatitis (hepatic failure) which include viral hepatitis, drug-induced hepatitis, nonalcoholic fatty liver disease, alcoholic hepatitis, autoimmune hepatitis and the like is provided and has remarkable social benefits.

Description

technical field [0001] The invention relates to the field of drug research and development, and specifically discloses the use of compound HUBIN-1 in the preparation of drugs for the prevention and / or treatment of liver inflammatory diseases. Background technique [0002] Liver inflammatory disease is a common clinical disease at present. From the perspective of etiology, it can be classified into viral hepatitis, drug-induced hepatitis, steatohepatitis, alcoholic hepatitis and autoimmune hepatitis. my country is a country with high prevalence of hepatitis B virus (HBV) infection, and there are about 30 million hepatitis B patients in the country. According to the priority of onset and the duration of the disease, it can be divided into acute hepatitis, chronic hepatitis, severe hepatitis (liver failure) and so on. Due to the lack of appropriate and effective intervention and treatment measures, some patients with acute hepatitis and chronic hepatitis can rapidly develop in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4178A61P1/16A61P31/12
CPCA61K31/4178
Inventor 陈智刘艳宁朱海红
Owner ZHEJIANG UNIV
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