Thiobacillus thioparus and high-activity culture method

A technology of Thiobacillus thioexans and Thiobacillus, which is applied in the field of Thiobacillus thioexans and high-activity cultivation, can solve the problems of low number of viable bacteria, low cost, short cycle, etc., to reduce the osmotic pressure difference, increase the number of viable bacteria, low cost effect

Inactive Publication Date: 2017-05-10
NANJING NORMAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

Ultimately lead to long cell culture cycle, low number of viable bacteria, etc.
[0003] As the use of microbial metabolic mechanisms to purify sulfide-containing waste gas and wastewater research is gradually carried out in the academic circle, the problems of long culture cycle and low number of viable bacteria make it difficult for the bacteria to adapt to industrialization. High efficiency, short cycle, and low cost characteristics

Method used

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  • Thiobacillus thioparus and high-activity culture method

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example 1

[0026] Prepare A 1 , B 1 Two media, A 1 : Na 2 S 2 o 3 9g / L, KH 2 PO 4 3g / L, K 2 HPO 4 4g / L, MgSO 4 .7H 2 O 0.7g / L, NH 4 Cl 0.4g / L, disodium oxalate tetraacetate 0.5g / L, ZnSO 4 .7H 2 O 0.22g / L, CaCl 2 0.05g / L, MnCl 2 .4H 2 O 0.05~g / L, FeSO 4 .7H 2 O 0.05g / L, (NH 4 ) 6 Mo 7 o 24 0.01g / L, CuSO 4 .5H 2 O 0.01g / L, CoCl 2 .6H 2 O0.01g / L, pH6.8; B 1 Containing the thalline protective agent of the present invention, wherein the autotrophic medium component is: Na 2 S 2 o 3 9g / L, KH 2 PO 4 3g / L, K 2 HPO 4 4g / L, MgSO 4 .7H 2 O 0.7g / L, NH 4 Cl 0.4g / L, disodium oxalate tetraacetate 0.5g / L, ZnSO 4 .7H 2 O 0.22g / L, CaCl 2 0.05g / L, MnCl 2 .4H 2 O0.05~g / L, FeSO 4 .7H 2 O 0.05g / L, (NH 4 ) 6 Mo 7 o 24 0.01g / L, CuSO 4 .5H 2 O 0.01g / L, CoCl 2 .6H 2 00.01g / L; add and account for above-mentioned autotrophic culture medium mass percentage again and be: 2wt% trehalose+0.1wt% polyethylene glycol 4000 (PEG)+0.3wt% sucrose+0.2wt%NaCl; Obtain mixed cult...

example 2

[0029] Prepare A separately 2 , B 2 Two media, A 2 : Na 2 S 2 o 3 10g / L, KH 2 PO 4 4g / L, K 2 HPO 4 5g / L, MgSO 4 .7H 2 O0.8g / L, NH 4 Cl 0.5g / L, disodium oxalate tetraacetate 0.6g / L, ZnSO 4 .7H 2 O0.32g / L, CaCl 2 0.07g / L, MnCl 2 .4H 2 O 0.06g / L, FeSO 4 .7H 2 O 0.06g / L, (NH 4 ) 6 Mo 7 o 24 0.02g / L, CuSO 4 .5H 2 O 0.02g / L, CoCl 2 .6H 2 O 0.02g / L, pH6.9, B 2 Containing the thalline protective agent of the present invention, wherein the autotrophic medium component is: Na 2 S 2 o 3 10g / L, KH 2 PO 4 4g / L, K 2 HPO 4 5g / L, MgSO 4 .7H 2 O 0.8g / L, NH 4 Cl 0.5g / L, disodium oxalate tetraacetate 0.6g / L, ZnSO 4 .7H 2 O 0.32g / L, CaCl 2 0.07g / L, MnCl 2 .4H 2 O 0.06g / L, FeSO 4 .7H 2 O 0.06g / L, (NH 4 ) 6 Mo 7 o 24 0.02g / L, CuSO 4 .5H 2 O 0.02g / L, CoCl 2 .6H 2 O 0.02g / L; then add 3wt% trehalose+0.2wt% polyethylene glycol 4000 (PEG)+0.4wt% sucrose+0.3wt%NaCl to account for the above-mentioned autotrophic medium mass percentage; Obtain the mixed cu...

example 3

[0032] Prepare A separately 3 , B 3 Two media, A 3 : Na 2 S 2 o 3 10g / L, KH 2 PO 4 5g / L, K 2 HPO 4 5g / L, MgSO 4 .7H 2 O0.9g / L, NH 4 Cl 0.6g / L, disodium oxalate tetraacetate 0.6g / L, ZnSO 4 .7H 2 O0.32g / L, CaCl 2 0.08g / L, MnCl 2 .4H 2 O 0.08g / L, FeSO 4 .7H 2 O 0.08g / L, (NH 4 ) 6 Mo 7 o 24 0.03g / L, CuSO 4 .5H 2 O 0.03g / L, CoCl 2 .6H 2 O 0.03g / L, pH7.0, B 3 Containing the thalline protective agent of the present invention, wherein the autotrophic medium component is: Na 2 S 2 o 3 10g / L, KH 2 PO 4 5g / L, K 2 HPO 4 5g / L, MgSO 4 .7H 2 O 0.9g / L, NH 4 Cl 0.6g / L, disodium oxalate tetraacetate 0.6g / L, ZnSO 4 .7H 2 O 0.32g / L, CaCl 2 0.08g / L, MnCl 2 .4H 2 O 0.08g / L, FeSO 4 .7H 2 O 0.08g / L, (NH 4 ) 6 Mo 7 o 24 0.03g / L, CuSO 4 .5H 2 O 0.03g / L, CoCl 2 .6H 2 O 0.03g / L; add and account for above-mentioned autotrophic culture medium mass percentage again and be: 4wt% trehalose+0.3wt% polyethylene glycol 4000 (PEG)+0.5wt% sucrose+0.4wt%NaCl; Obtai...

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Abstract

The invention discloses thiobacillus thioparus and a high-activity culture method. The category name is thiobacillus thioparus and the Latin name of the strain is thiobacillus thioparus; the preservation date is July 11, 2016 and the registering number in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.12756. An autotrophic culture medium and a protective agent are prepared into a mixed culture medium in a container and the liquid filling amount of the mixed culture medium is 10%-20% of the volume capacity of the container; the culture medium is sterilized; thiobacillus thioparus is inoculated according to the amount of 2%-5% of the volume ratio of the mixed culture medium; the temperature is controlled to be 28 DEG C-30 DEG C and the rotary speed of a shaking table is 160rpm/min-180rpm/min; and the strain is cultured for 48h-72h. By adopting the method to culture thiobacillus thioparus, a protein salting-in phenomenon of cell membranes and cell walls of thiobacillus thioparus, generated by inorganic salt in the autotrophic culture medium, can be effectively avoided, so that the death rate of thalli is reduced and the activity of the thalli is improved. Furthermore, the method has the characteristics of simplicity in operation, low cost, remarkable effect and the like.

Description

technical field [0001] The invention relates to the field of biochemical industry, in particular to a method for cultivating Thiobacillus thioexhausen and its high activity. Using the method to cultivate Thiobacillus thioexhausen can greatly reduce the death rate of bacteria and obviously increase the number of viable bacteria in a unit of bacterial liquid. Background technique [0002] The traditional culture method of Thiobacillus thioexhausens is based on the growth requirements of chemoautotrophic bacteria, using simple inorganic salts such as thiosulfate as carbon and nitrogen sources. Thiobacillus thiaphragans grows slowly in this type of medium, and it takes at least 5 days to see round colonies with a diameter of less than 1 mm in the medium. Thiobacillus thioexans forms a mucous membrane with free sulfur in this type of medium. After standing for a long time, the sulfur produced by metabolism will form white or light yellow precipitates in the medium. This class is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 杨宸吴勇
Owner NANJING NORMAL UNIVERSITY
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