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Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof

A technique of immobilizing small balls and sulfates, applied in the direction of fixing on or in inorganic carriers, fixing on/in organic carriers, chemical instruments and methods, etc., can solve the problems of high cost and low processing efficiency , to achieve the effect of low cost, high removal rate and low leaching toxicity

Inactive Publication Date: 2017-05-10
HUNAN UNIV
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  • Claims
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to develop a high-efficiency, reusable immobilized spherical part

Method used

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  • Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof
  • Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof
  • Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof

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Embodiment 1

[0026] A kind of nutrient source of the present invention embodiment 1 of the present invention immobilizes sulfate-reducing bacteria pellets to process Cu, Zn, Pb, Cd four kinds of metals in Xiangjiang sediment, comprising the following steps:

[0027] a. Add the collected Xiangjiang sediment sample filtered through a 100-mesh sieve to the immobilized pellet at a ratio of 5:1 into a 150mL serum bottle containing bacterial culture medium and place it in a constant temperature incubator at 37°C under ambient conditions. After shaking at 150rpm for 5 days, the pellets were collected by filtration, the suspension was centrifuged at 3000rpm for 20min, and sediment samples were collected and freeze-dried for continuous extraction of BCR;

[0028] b. Take 0.2 g of the freeze-dried sample after centrifugation and put it into a 50 mL polytetrafluoroethylene centrifuge tube, add 20 mL of 0.11 mol / L acetic acid and shake it at a constant temperature for 16 hours, then add 10 mL of deioni...

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Abstract

The invention relates to a preparation method and application of nutrient source immobilized sulfate reducing bacterium globules. Immobilized globules take polyvinyl alcohol and sodium alginate as carriers, a saturated boric acid calcium chloride solution as a cross-linking agent and sodium lactate as a nutrient source. The preparation method comprises the specific steps: firstly, dissolving the polyvinyl alcohol and the sodium alginate in a water bath at a constant temperature; then, adding active carbon, calcium carbonate, silicon dioxide and free bacterium liquid; sucking a mixed solution by an injector and dropwise adding the mixed solution into the saturated boric acid calcium chloride solution; crossly linking for 24h to form the globules; washing the globules with sterile physiological saline and putting the globules into a sodium lactate solution for 24h; after washing, freezing and drying to obtain the nutrient source immobilized sulfate reducing bacterium globules. In the application of the nutrient source immobilized sulfate reducing bacterium globules prepared by the invention, the nutrient source immobilized sulfate reducing bacterium globules have a good removing effect on four types of heavy metal including Cu, Zn, Pb and Cd in sediment of Xiang River; the treatment is stable and the leaching rate is low. The material is convenient to separate, can be repeatedly utilized and does not cause secondary pollution; the nutrient source immobilized sulfate reducing bacterium globules has good economic benefits and a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of heavy metal pollution treatment, and in particular relates to a small ball made of immobilized sulfate-reducing bacteria cohesive nutrient source to stabilize the deposits polluted by four heavy metals of copper, zinc, lead, and chromium, and the small ball can be repeated. availability. Background technique [0002] Heavy metal pollution in sediments has great harm to environmental ecosystems and people's lives. With the intensive industrialization and urbanization process, these heavy metals cannot be biodegraded after entering the aquatic environment through the food chain, and accumulate in organisms, destroying their metabolic activities and endangering human health and ecosystem balance. Moreover, the tiny particles in the sediment affect the migration of heavy metals to form stable compounds, making the metals persist for a long time, and the toxic pollution is becoming more and more serious. The...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C12N11/04C02F3/34C02F101/20C02F101/22
CPCC12N11/14C02F3/34C02F2101/20C02F2101/22C12N11/04C12N11/08C12N11/10
Inventor 代丽花李欣张长曾光明刘云国吴幼娥田亚灵
Owner HUNAN UNIV
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