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SNP molecular marker related to daily gain of Duroc and application thereof

A marker and trait technology, applied in the field of molecular biology, can solve the problems of accelerating genetic progress, time-consuming and labor-intensive problems

Inactive Publication Date: 2017-05-10
广西柯新源原种猪有限责任公司 +6
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this work is time-consuming and labor-intensive, so it is necessary to develop a fast, simple and efficient molecular breeding method in production, which will speed up the genetic progress of the daily gain of the added line Duroc

Method used

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  • SNP molecular marker related to daily gain of Duroc and application thereof
  • SNP molecular marker related to daily gain of Duroc and application thereof
  • SNP molecular marker related to daily gain of Duroc and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0021] In order to explore the genetic mechanism of the added-line Duroc daily gain and develop a fast and efficient molecular breeding technology related to the daily gain selection, the present invention uses 83 Duroc populations to analyze through genome-wide association (GWAS) and other methods, found and identified a SNP site that affects the daily weight gain of the Canadian Duroc. On this basis, the present invention also constructs a fast and efficient molecular typing technology. Through the in-depth study of the phenotypes corresponding to different genotypes, the genotypes related to the daily gain were determined. Through the selection of the genotypes, the genetic progress of the daily gain of the added line of Duroc can be accelerated.

[0022] 1. Experimental animals 83 animals were added to the Duroc experimental group, which came from the National Core Breeding Farm of Duroc.

[0023] The daily weight gain phenotype value of 83 plus line Duroc is as follows f...

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PUM

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Abstract

The invention discloses a SNP molecular marker related to daily gain of Duroc and an application thereof. Through genome-wide association study (GWAS), an SNP site (ASGA0056780) related to the daily gain of Duroc is identified at the No. 13 chromosome of a pig; the GG type individual at the site has faster daily gain. The invention also provides a method for detecting the SNP molecular marker at the same time; the Taq probe can judge the site rapidly. The method can exactly and rapidly classify the ASGA0056780 with low cost, and provide technical support to the establishment of the breeding method of the Duro type daily gain molecular marker.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a SNP site that affects the daily gain traits of plus-line Duroc and its application in genetic improvement of breeding pigs. Background technique [0002] Duroc is native to New Jersey and New York in the eastern part of the United States. The main parent is a hybrid of Duroc from New York State and Jersey Red from New Jersey. It was originally called Duroc Jersey and later collectively called Duroc. Hejia Duroc; Duroc produced in Taiwan, my country has been cultivated into its own style, so it is called Taiwan Duroc or Taiwan Duroc. [0003] Duroc pigs have the advantages of good adaptability, no stress sensitivity, easy feeding and management, fast weight gain, high feed remuneration (feed-to-meat ratio 2.8-3.2:1), good carcass quality, and high lean meat percentage. It is suitable for factory pig raising and farmer raising. [0004] Pig daily gain is an important eco...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 龙毅万文峰廖玲玲李亮吴有林周盛昌肖丽萍敖大国颜勇周亚丽刘自芹
Owner 广西柯新源原种猪有限责任公司
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