Fluorescence compensation method for flow cytometer

A technology of flow cytometer and compensation method, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems that wavelength correlation cannot truly reflect the corresponding relationship between excitation light and fluorophore, fluorescence spectrum overlap, measurement error, etc. Achieve the effect of solving fluorescence detection and analysis compensation

Inactive Publication Date: 2017-05-10
BEIJING INFORMATION SCI & TECH UNIV
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Problems solved by technology

However, the light intensity is wavelength-dependent and cannot truly reflect the correspondence between excitation light and fluorophores
[0005] Therefore, in order to solve the problem of measurement error caused by the overlap of fluorescence spectra in the process of multicolor analysis of flow cytometry, a method of fluorescence detection and analysis compensation for flow cytometry that can effectively solve the above problems is needed

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  • Fluorescence compensation method for flow cytometer
  • Fluorescence compensation method for flow cytometer
  • Fluorescence compensation method for flow cytometer

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Embodiment Construction

[0022] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. In the drawings, the same reference numerals represent the same or similar components, or the same or similar steps.

[0023] The objects and functions of the present invention and methods for achieving the objects and functions will be clarified by referring to the exemplary embodiments. However, the present invention is not limited to the exemplary embodiments disclosed below; it can be implemented in various forms. The essence of the description is only to help those skilled in the relevant art comprehensively understand the specific details of the present invention.

[0024] The present invention is described in detail in conjunction with schematic diagrams. When describing the embodiments of the present invention in detail, for the convenience of explanation, the cross-sectional view showing the structure of the device will not be partially enlarged acc...

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Abstract

The invention provides a fluorescence compensation method for a flow cytometer. The fluorescence compensation method comprises the following steps: step (1) performing single positive sample testing; step (2) calculating fluorescence leakage coefficients and fluorescence leakage matrixes of each channel; step (3) calculating the fluorescence leakage matrixes, and according to the fluorescence leakage matrix K<-1>, obtaining a compensation matrix K<-1>; step (4) performing compensation correction; step (5) performing multi-color analysis test: testing samples colored by a multi-color fluorescence dye, and recording the signal amplitude of each detecting channel; step (6) performing fluorescence compensation.

Description

technical field [0001] The present invention relates to a fluorescence detection and spectral analysis method of a flow cytometer, in particular to a fluorescence compensation method of a flow cytometer. Background technique [0002] Flow cytometer is a new high-tech instrument integrating laser technology, electronic physics technology, photoelectric measurement technology, electronic computer technology, cell fluorescence chemistry technology and monoclonal antibody technology. For single cells or other particles in high-speed, straight-line flow in suspension, high-speed one-by-one multi-parameter quantitative analysis can be realized by detecting scattered light signals and (or) labeled fluorescent signals. It has a wide range of applications in the fields of cell biology, cell cycle dynamics, immunology, hematology and oncology. [0003] The principle of the flow cytometer is that the single cell suspension is dyed with a specific fluorescent dye and then enters the fl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64
CPCG01N15/1429G01N15/1434G01N21/6486
Inventor 祝连庆张文昌潘志康董明利娄小平郭阳宽刘超
Owner BEIJING INFORMATION SCI & TECH UNIV
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