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Method for measuring residual quantity of glyphosate in soil employing liquid chromatography-high-resolution mass spectrometer

A technology of high-resolution mass spectrometry and medium glyphosate, which is applied in the field of determination of glyphosate residues in soil by liquid chromatography high-resolution mass spectrometry, can solve the problems of heavy workload, low accuracy and sensitivity, and long time, and achieve The effect of eliminating interference, improving accuracy and quantitative reliability

Active Publication Date: 2017-05-10
NANJING UNIV +1
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Problems solved by technology

[0005] The present invention aims at the problems that the determination method of glyphosate residue in soil is too complicated, heavy workload, long time, and low in accuracy and sensitivity in the prior art, and provides a liquid chromatography high-resolution mass spectrometry method for measuring soil Method for Glyphosate Residues in China

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  • Method for measuring residual quantity of glyphosate in soil employing liquid chromatography-high-resolution mass spectrometer
  • Method for measuring residual quantity of glyphosate in soil employing liquid chromatography-high-resolution mass spectrometer

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Embodiment Construction

[0024] Collect the soil at a depth of 10 cm in a certain farmland as a sample to be tested, and sample liquid chromatography high-resolution mass spectrometry to determine the glyphosate residue in the soil, including the following steps:

[0025] 1) Potassium hydroxide extraction of glyphosate residues in soil samples

[0026] Weigh 5.0g of soil sample into a plastic centrifuge tube, add 20ml of 20% potassium hydroxide, carry out vibration and centrifugation treatment, shake and mix, ultrasonically extract for 30min, then shake and extract for 30min, remove the supernatant and extract once with 25ml of dichloromethane , and then extracted once with 25ml of dichloromethane, and the aqueous layer solution was to be purified; the extract was extracted twice with 50ml of dichloromethane, centrifuged at 5000r / min for 5min, and the aqueous layer was collected to be purified.

[0027] 2) Dispersive solid phase extraction purification of sample extract

[0028] Take the aqueous laye...

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Abstract

The invention discloses a method for measuring the residual quantity of glyphosate in soil employing a liquid chromatography-high-resolution mass spectrometer. The method comprises the following steps of (1) potassium hydroxide extraction of the residual glyphosate in a soil sample; (2) disperse solid-phase extraction and purification of a sample extract solution; (3) detection by using the liquid chromatography-high-resolution mass spectrometer; and (4) result analysis, which comprises qualitative analysis and quantitative analysis. The residual glyphosate in the soil is extracted by using the potassium hydroxide, an organic matter in the soil is removed by using dichloromethane and purification is carried out by using a CAX column; and qualitative and quantitative analysis is carried out on an accurate mass number of the glyphosate in the soil sample by using the liquid chromatography-high-resolution mass spectrometer, the interference of other non-target compounds can be eliminated and the qualitative accuracy and the quantitative accuracy of the glyphosate are further improved.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to a method for measuring glyphosate residues in soil by liquid chromatography high-resolution mass spectrometry. Background technique [0002] Soil is an important part of the natural ecological environment, it is a complex multi-media carrier, and it is also an important attribute of herbicides. The chemical name of glyphosate is N-phosphine hydroxymethylglycine. Because of its high efficiency and low toxicity, it has become one of the widely used herbicides. Since it was introduced to the market, it has been widely used for the control of various weeds. Control of weeds in farmland; glyphosate is continuously enriched in the environment and organisms, and enters the body through food and drinking water, causing harm to the human body; the analysis report of Greenpeace also pointed out that the widespread use of the herbicide glyphosate is unsafe Yes, it has effec...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 任洪强许柯
Owner NANJING UNIV
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