Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for filtering DNA base bias in DNAse high-throughput sequencing data

A technology of sequencing data and tendency, applied in the fields of biochemical equipment and methods, sequence analysis, microbial determination/inspection, etc., can solve the problems of unfavorable and influence of DNA protein binding site identification, etc.

Active Publication Date: 2019-10-18
HARBIN ENG UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, it has recently been found that DNase nuclease has a certain DNA base tendency when cutting DNA, which will have an adverse effect on the recognition of DNA protein binding sites

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for filtering DNA base bias in DNAse high-throughput sequencing data
  • A method for filtering DNA base bias in DNAse high-throughput sequencing data
  • A method for filtering DNA base bias in DNAse high-throughput sequencing data

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in conjunction with the accompanying drawings.

[0025] As a new technology for DNA protein binding site detection, DNase-Seq technology has many outstanding advantages. Due to its lack of specificity, DNase-Seq can simultaneously detect the binding sites of multiple DNA proteins in the whole genome at one time; due to the one-time detection of the binding sites of multiple DNA proteins, DNase-Seq greatly improves the detection efficiency and It reduces the cost of detection and makes it possible to detect DNA protein binding sites on a large scale; since the starting position of sequencing is the position of enzyme cleavage, DNase-Seq can detect DNA protein binding sites with a resolution of up to a single base.

[0026] However, it has recently been found that DNase nucleoclease has a certain DNA base tendency when cutting DNA, which will have an adverse effect on the recognition of DNA protein binding sites. The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of molecular bio-information detection and analysis, and particularly relates to a method used for filtering a DNA base tendentiousness deviation in DNase high-throughput sequencing data and capable of effectively improving detection information accuracy of the DNase high-throughput sequencing data. The method comprises the steps of (1) obtaining a DNA base of an enzyme cutting site region of DNase-Seq experiment data; (2) obtaining DNA base tendentiousness of the DNase-Seq experiment data; and (3) removing the DNA base tendentiousness. Through the method, the DNA base tendentiousness deviation contained in the DNase high-throughput sequencing data can be accurately filtered to generate a more accurate DNase-Seq sequencing result so as to provide data guarantee for subsequent higher-level application analysis.

Description

technical field [0001] The invention belongs to the field of molecular biological information detection and analysis, and in particular relates to a method for effectively improving the accuracy of detection information of DNase high-throughput sequencing data and filtering out DNA base tendency deviations in DNase high-throughput sequencing data. Background technique [0002] At present, the detection of DNA protein binding sites mainly adopts Chromatin Immunoprecipitation (Chromatin Immunoprecipitation, ChIP). The ChIP-Seq technology, which combines the results of ChIP experiments with high-throughput sequencing technology, can effectively detect the binding sites of target functional proteins on DNA on a genome-wide scale. The principle of ChIP-Seq is: firstly, the DNA fragments bound to the target protein are enriched by using the enzyme that specifically binds to the target protein through chromatin immunoprecipitation (ChIP), and then purified and library constructed. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G16B30/00C12Q1/68
Inventor 冯伟兴贺波宋艳霞徐斯文赵森陈多娇刘欢
Owner HARBIN ENG UNIV