A kind of extracting method of total saponins of Lonicera japonica
An extraction method and a technology of total saponins, which are applied in the field of extraction of total saponins from Lonicera japonica, can solve problems such as high polarity, difficulty in extraction, and large molecular weight of saponin compounds
Inactive Publication Date: 2020-07-14
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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[0004] The purpose of the present invention is to provide a method for extracting total saponins of Lonicera japonica, which aims to solve the problem that saponin compounds usually have relatively large molecular weight and high polarity and are difficult to extract
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[0010] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0011] The present invention investigates the effects of ethanol concentration (%), temperature (° C.), time (min), and solid-liquid ratio (W / V) on the extraction of total saponins from Lonicera japonica through orthogonal experiment design. Based on the extract rate of total saponins, the optimal conditions for ethanol reflux were obtained: 80% ethanol, the ratio of solid to liquid (W / V) was 1:14, and reflux at 70°C for 105 minutes. Under this condition, the extraction rate can reach 9.84%. The antibacterial activity of the total saponins was detected by the microdilution method, and the results showed that the total sapo...
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The invention discloses a method for extracting total saponins of lonicera rupicola. According to the method for extracting total saponins of lonicera rupicola, the optimal condition of ethanol backflow extraction is 80%, a material-to-liquid ratio is 1:14, and backflow is implemented for 105 minutes at 70 DEG C. By adopting the method, the single backflow time can be shortened, the backflow times can be increased, and whether the extraction rate can be further increased or not needs to be further researched. At present, most people select that traditional Chinese medicine components of a sample are purified and enriched by using macroporous resin directly after ethanol backflow extraction; since a decoction is relatively high in concentration possibly, a part of liposoluble substances and other impurities can be removed if extraction is implemented firstly, and thus the adsorption rate of the macroporous resin can be increased. AB-8 macroporous resin for extracting saponin components in plants is selected for most tests at present. MIC (Minimum Inhibitory Concentration) tests show that the saponin component in the lonicera rupicola has certain antibacterial activity on 14 standard strains, and has values of further research.
Description
technical field [0001] The invention belongs to the technical field of extracting total saponins, and in particular relates to a method for extracting total saponins of Lonicera japonica. Background technique [0002] Lonicera rupicola (Lonicera rupicola Hook.f.et Thomson.) is an evergreen vine plant belonging to the family Lonicaceae. Mainly distributed in Gansu (Lintan), southern Ningxia, western Sichuan, northwestern Yunnan, Tibet (Linzhi, Cuona). Growing in alpine shrub meadows, edges of rocky beaches, forest margins, river beach grasslands or hillside shrubs. Mild in taste, slightly sweet, cold in nature. Heat-clearing and toxic substances removing, dredging wind and dredging collaterals. For febrile disease fever, pyretic toxin bloody dysentery, carbuncle sores, rheumatism-heat arthralgia, joint redness, swelling and heat pain. Its stems, leaves and flowers are used as medicine, which has the functions of clearing heat and detoxifying, antibacterial and anti-inflam...
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IPC IPC(8): A61K36/355A61P31/04
CPCA61K36/355A61K2236/333
Inventor 陈朝喜
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES