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Bacillus amyloliquefaciens resistant to powdery mildew of melon crops and high in spore yield

A technology for dissolving starch spores and powdery mildew, applied in the field of microorganisms, can solve problems such as high requirements for growth environment, poor stress resistance, and inability to be popularized and applied in batches.

Active Publication Date: 2017-05-24
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common bacteria, yeast and actinomycetes have poor stress resistance and high requirements for growth environment, so they cannot be popularized and applied in batches

Method used

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  • Bacillus amyloliquefaciens resistant to powdery mildew of melon crops and high in spore yield
  • Bacillus amyloliquefaciens resistant to powdery mildew of melon crops and high in spore yield
  • Bacillus amyloliquefaciens resistant to powdery mildew of melon crops and high in spore yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Purification and cultivation of the starting strain of Bacillus amyloliquefaciens

[0014] A single colony of the originating strain Bacillus amyloliquefaciens KN-52 (naturally screened from the soil of Wuhan vegetable planting base) was picked, and inserted into a mixture of 1% peptone, 0.5% yeast extract powder, 1% sodium chloride, pH7.2- In the simple culture medium composed of 7.5, culture on a constant temperature shaker at a temperature of 32°C and 180rpm for 16-20 hours, take 10ml of fermentation broth, centrifuge at 4°C and 6000r / min, and collect the bacterial slurry for later use;

[0015] (2) Preliminary screening: normal temperature and pressure plasma mutagenesis method

[0016] Dissolve the bacteria prepared in step (1) with 10ml of sterile normal saline and shake evenly, take 10μl and apply it on a sterilized metal slide, place the slide on the stage, adjust the height of the stage, and set Bacillus amyloliquefaciens was irradiated after a good treatm...

Embodiment 2

[0028] Example 2 Verification of the field inhibitory effect of Bacillus amyloliquefaciens KN-527 on powdery mildew of melon.

[0029] 1. Materials

[0030] The test agent: Bacillus amyloliquefaciens KN-527, and the traditional powdery mildew control agent was selected as the chemical pesticide.

[0031] Test crops: melon

[0032] Control object: powdery mildew

[0033] A total of 3 treatments were set up in the experiment:

[0034] (1) control group: clear water treatment;

[0035] (2) treatment group: the bacillus amyloliquefaciens diluted 1000, 2000 and 3000 times of 0.1 million CFU / g

[0036] (3) 50% carbendazim wettable powder;

[0037] The above treatments were carried out in the muskmelon greenhouse of the scientific research base. The side vines of the muskmelon tested were removed before spraying, and the spray was applied for the first time at the early stage of the disease, and then sprayed again after 7 days, and the liquid medicine was evenly sprayed on the ...

Embodiment 3

[0054] Example 3 Verification of the field effect of Bacillus amyloliquefaciens KN-527 as a biological fertilizer on muskmelon.

[0055] The test agent: Bacillus amyloliquefaciens KN-527

[0056] Test crops: melon

[0057] Nutrient solution: Israeli Polyfood all-element nutrient solution

[0058] experiment method:

[0059] The melons after one week of planting were divided into two groups. Group A was given nutrient solution + water, and group B was given nutrient solution + Bacillus amyloliquefaciens wettable powder.

[0060] The plant growth, fruit growth, yield and quality were inspected at the vine-pulling stage, melon-setting stage, and melon-expanding stage.

[0061] Table 3 Field effect verification of Bacillus amyloliquefaciens as biological fertilizer on melon

[0062]

[0063] The test results show that Bacillus amyloliquefaciens KN-527 can be used as a biological fertilizer, which can significantly promote the growth of muskmelon plants, enhance the ability ...

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Abstract

The invention discloses bacillus amyloliquefaciens KN-527 which is induced through normal-pressure room-temperature plasmas to have a high spore yield, and a preservation number of the bacillus amyloliquefaciens KN-527 is CCTCC (China Center for Type Culture Collection) NO: M2015709. Fermented spore count of the bacterial strain is increased by 161% in comparison with that before mutagenesis, a spore forming rate is high, heat resistance is good, remarkable disease resistance is achieved on melon powdery mildew, and the control effect can be 95% or higher. The bacillus amyloliquefaciens KN-527 can be applied to melon crops such as cucumbers, summer squashes, pumpkins, bitter gourds and watermelons. According to a melon field test, conventional fertilization is performed on a microbial agent produced by the bacterial strain, so that plant grows vigorously, capacity of developing lateral branches is strong, and leaf color is bright green.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacillus amyloliquefaciens, and also relates to a microbial agent for resisting powdery mildew of melon crops. Background technique [0002] Powdery mildew is a common disease of melon crops. There are 3 genera and 6 species of powdery mildew. Physiological race. Powdery mildew mainly affects leaves, but also infects petioles, vines and melons. At the beginning of the disease, small white mildew spots (that is, pathogenic mycelium and conidia) will appear on the leaf surface, and then expand into mildew spots. When the conditions are suitable, the mildew spots will expand rapidly and connect to each other, and white powder will spread. The entire leaf is covered, causing the leaves to become brittle, and the mildew on the lesion becomes grayish white in the later stage, and small black grains grow on it, which is the closed capsule of the pathogen. If ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07C12R2001/20
Inventor 周莉潘渝刘华梅程治国袁善奎
Owner WUHAN KERNEL BIO-TECH CO LTD
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