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PH responsive reversible fluorescent probe and preparation method thereof

A fluorescent probe and fluorescent technology, applied in fluorescence/phosphorescence, material analysis through optical means, material excitation analysis, etc., can solve the problems that liquid acid cannot be loaded, limit the application range of basic fluorescent molecules, etc., and achieve simple raw materials Effects of composition, increased utilization efficiency, and simple synthesis method

Active Publication Date: 2017-05-24
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liquid acid can be used to provide a proton environment to quench fluorescence, but liquid acid cannot be loaded on the carrier, which limits the application range of basic fluorescent molecules

Method used

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  • PH responsive reversible fluorescent probe and preparation method thereof
  • PH responsive reversible fluorescent probe and preparation method thereof

Examples

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Embodiment 1

[0022] A pH-responsive reversible fluorescent probe, made of the following raw materials in parts by weight: 0.01 part of fluorescent molecule (fluorescein isothiocyanate), quencher (H 3 PMo 12 o 40 ) 99.99 copies. Add the fluorescent molecule and quencher to water, stir to dissolve, and then remove the water to obtain a fluorescent probe that can respond sensitively to pH changes. The probe has very weak fluorescence at pH ≤ 6, and has Strong fluorescence, and its fluorescence intensity can change reversibly with acidity and alkalinity.

Embodiment 2

[0024] A pH-responsive reversible fluorescent probe, made of the following raw materials in parts by weight: 0.2 parts of fluorescent molecule (2-naphthylamine), quencher (H 4 SiW 12 o 40 ) 99.8 copies. Add the fluorescent molecule and quencher to ethanol, stir to dissolve, and then remove the ethanol to obtain a fluorescent probe that can respond sensitively to pH changes. It has strong fluorescence, and its fluorescence intensity can change reversibly with acidity and alkalinity.

Embodiment 3

[0026] A pH-responsive reversible fluorescent probe, made of the following raw materials in parts by weight: 2 parts of fluorescent molecule (dichlorofluorescein), quencher (H 3 AsMo 12 o 40 ) 98 copies. Add the fluorescent molecule and quencher to methanol, stir to dissolve, and then remove the methanol to obtain a fluorescent probe that can respond sensitively to pH changes. It has strong fluorescence, and its fluorescence intensity can change reversibly with acidity and alkalinity.

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Abstract

Belonging to the technical field of molecular imaging, the invention in particular relates to a pH responsive reversible fluorescent probe and a preparation method thereof. The pH responsive reversible fluorescent probe comprises, by weight, 0.01%-20% of a fluorescent molecule and 80%-99.99% of a solid acid quenching agent, and the fluorescent molecule can fluoresce under an alkaline condition. The invention adopts simple raw material composition and a simple synthesis method to prepare the fluorescent probe with sensitive response to pH change, and because of the adding of solid acid, the utilization efficiency of the fluorescent molecule in actual operation is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of molecular imaging, and in particular relates to a pH-responsive reversible fluorescent probe and a preparation method thereof. Background technique [0002] Fluorescent probes have characteristic fluorescence at ultraviolet-visible-near-infrared, and their fluorescence properties (excitation and emission wavelengths, intensity, lifetime, polarization, etc.) change sensitively. Since fluorescent probes can fluoresce at the molecular level, their ability to fluoresce depends on the molecular structure of the fluorescent probe, and its fluorescence after intermolecular interactions with nucleic acids (DNA or RNA), proteins, or other macromolecular structures The properties will change, so fluorescent probes have been widely used in research fields such as single-cell imaging, studying the properties and behavior of macromolecules, and determining targeted genes. The fluorescence excited by a fluorescent pr...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李国良王军鹏张绘齐涛
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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